Multiplicación in vitro de Eucalyptus globulus mediante sistema de inmersión temporal

Abstract

SUMMARYWith the object of establishing mass production methods of Eucalyptus globulus, factors affecting its in vitro multiplication stage by temporary immersion were studied. For this, five microshoots per flask containing 250 mL of Murashige and Skoog medium were disposed. Factors such as immersion frequency (6, 12, 24, 48 h), immersion time (1, 2 and 3 min) and macronutrients concentration (25, 50 and 100 %) were studied. In a second stage, the addition into medium of 6-bencylaminopurine (0, 2.2 and 4.4 µM), polyvinylpyrrolidone (0, 250 y 500 mg L -1 ) and sucrose concentration (43.8 and 87.6 mM) was analyzed. A completely random design with factorial arrangement was used in each stage. Variables evaluated were number of new shoots per flask, dry and fresh weight rate and final pH of culture medium. The best rate of multiplication and less hyperhydration was obtained with immersion time of 2 min, a frequency of 12hours, macronutrients at 50 %, 2.2 µmol of 6-bencylaminopurine, 43.8 mM of sucrose and 250 mg L -1 of polyvinylpyrrolidone. Time and frequency of immersion interaction affects multiplication rate and microshoot hyperhydration. Use of 6-bencylaminopurine (2,2 µM) and polyvinylpyrrolidone (250 mg L -1 ) increases multiplication rate without influence on microshoot hyperhydration. A reduction on sucrose concentration decreases hyperhydration without affecting formation of new shoots.

Production of biomass and flavonoid of Gynura procumbens (Lour.) Merr shoots culture in temporary immersion system

“…We found that the long immersion frequency (12 h) have better result than short immersion frequency (3h). The similar result also shown in in vitro multiplication of Eucalyptus globulus , which was obtained the best multiplication in immersion time 2 min and immersion frequency 12 h [48].…”

Temporary Immersion System for Micropropagation of Tree Species: a Bibliographic and Systematic Review

“…The frequency and the immersion time are the main factors that influence the proliferation and hyperhydration of shoots, and this physiological disorder can occur by the formation of an aqueous film around the tissues that may interfere with gas exchanges between the outer tissue and the cell surface, since gas diffusion rates are about 10.000 times slower in water than in air (González et al, 2011;Jackson, 2003), a fact that corroborates the results of Chakrabarty et al (2003), reported above.…”

“…In Tectona grandis L. it was observed the presence of hyperhydric shoots in the highest concentration of BA used (6.6 μM), which impaired shoots proliferation in TIS (Quiala et al, 2012). In E. globulus, the highest percentage of hyperhydration (61.9%) occurred in the treatment with 4.4 uM BA when compared to treatments with 2.2 (51,4%) and without BA (45,3%) (González et al, 2011). In addition to higher concentration of cytokinins in culture medium, it should be taken into account that prolonged exposure times to BA can also lead to a physiological depletion of the material, thus reducing the rate of shoot proliferation in successive subcultures.…”

“…In Pistachio vera 'Siirt' the increase of the immersion frequency from 8 hours to 16 and 24 hours not only increased the percentage of proliferation (85 and 88%, respectively), but also prevented the hyperhydricity of the shoots (0%) (Akdemir et al, 2014). González et al (2011) observed that in Eucalytus globulus the hyperhydration of the shoots increases with the simultaneous increase of macronutrients concentration (25 to 100%) and immersion time (1 to 3 min), and also evidenced this phenomenon with the increase of immersion frequency (6 to 48 h).…”

Temporary immersion systems to improve alder micropropagation

“…When the frequency is reduced to 1 immersion/48 h, all these parameters decrease. In Eucalyptus globulus, González et al (2011), also showed that this immersion frequency (1 immersion/48 h) significantly decreased the multiplication rates, which was due to a reduction in the supply of nutrients.…”