Multiplex real-time PCR for detection and quantification of mycotoxigenic Aspergillus, Penicillium and Fusarium

Changes in Fungal Population and Aflatoxin Levels and Assessment of Major Aflatoxin Types in Stored Peanuts (Arachis hypogaea Linnaeus)

“…Determination of the level of fungal infection of a foodstuff as well as establishing the occurrence of common species is important as the fungal population and diversity could give an indication of the food quality and the presence and types of mycotoxins (Suanthie, Cousin, & Woloshuk, 2009). Aflatoxin producing fungi occur in three sections of the genus Aspergillus, but section Flavi contains the greatest number of potential aflatoxin producers (Pildain et al, 2008).…”

Culturable Fungi in Brown Bear Cave Dens

“…Penicillium taxa are also characterized by their ability to synthesize mycotoxins, which can consequently be found in underground objects [4,24]. As they intoxicate animal organisms via their alimentary and respiratory systems or by dermal contact, mycotoxins have an adverse effect on the health of exposed humans and animals [25]. In the Nad Pisaną den, the observed concentration of spores was at the level of 6,500 CFU/m 3 , and in the one Nad Jaworzynką (when the female and her young were absent from the den), the CFU/m 3 value reached nearly 6,000.…”

“…There is scant communication about the issue in scientifi c literature. Some authors fi rmly state that the incidence of some fungi species in the atmosphere of underground objects may negatively affect the health of the animal species living there, with bats making one example and the foremost reason being the ability of fungi to produce mycotoxins, or to infect the animal respiratory system, resulting in mycoses or other disorders [25,27]. Investigations carried out in the hibernation sites of bats have demonstrated that -apart from a number of Penicillium species capable of producing mycotoxins -the spores of Aspergillus spp.…”

Multiplex Real‐Time PCR Method for Detection and Quantification of Mycotoxigenic Fungi Belonging to Three Different Genera

“…Real‐time PCR involving TaqMan probes has been used for specific detection and reliable quantification of fungal pathogens (Bluhm and others 2004; Suanthie and others 2009; Vegi and others 2011). These probes are oligonucleotides with a reporter dye on the 5’ end and a quencher dye on the 3’ end which attach to specific DNA sequences during the PCR cycles (Geisen and others 2004; McDevitt and others 2004).…”

“…Studies by Bluhm and others (2004) and Suanthie and others (2009) have reported multiplex real‐time PCR methods to detect and quantify mycotoxigenic fungi. The former group targeted trichothecene‐ and fumonisin‐producing fungi in field‐collected barley and corn samples, whereas the latter group targeted fungi belong to the genera Aspergillus , Fusarium , and Penicillium in distiller's grain.…”