Multiplex ligation-dependent probe amplification (MLPA) for simultaneous detection of DNA from sunflower, poppy, flaxseed, sesame and soy allergenic ingredients in commercial food products

López-Calleja, Inés; García, Aina; Madrid, Raquel; Garcı́a, Teresa; Martı́n, Rosario; González, Isabel

doi:10.1016/j.foodcont.2016.06.014

Cited by 26 publications

(9 citation statements)

References 33 publications

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“…Ma developed and utilized stable isotopelabelled internal standard (SIIS) peptides (Ma et al, 2020). López-Calleja developed a method for determining the presence of low amounts of sesame in minced processed foods (López-Calleja et al, 2017). Fatima developed an indirect competitive ELISA to detect traces of sesame in food (Husain et al, 2010), Ecker and Maruyama developed a sandwich ELISA to determine the traces of sesame in food (Maruyama et al, 2016;Redl et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

The influence of pre-treatment methods and matrix effect on sesame ( Sesamum indicum ) sandwich ELISA detection

Wang

Wang³

et al. 2021

Food and Agricultural Immunology

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Food allergy is a serious health risk and severely affects the quality of life in individuals all over the globe. In recent years, there has been an evident increase in the number of food allergy cases due to the consumption of sesame. Hence, to develop a robust and reliable detection method for sesame allergen has become a high priority. In this research, a sandwich ELISA method was established to detect sesame allergens using rat and rabbit antiserums. The limit of detection for sandwich ELISA was estimated at 1 ng/mL, revealing high sensitivity. At the same time, it was found that the most suitable pre-treatment extract for this method was 0.1 M, pH 9.6 Na 2 CO 3 -NaHCO 3 containing 0.5% SDS. This sandwich ELISA method can sensitively and efficiently detect foods containing sesame ingredients, which can protect the sensitive individuals from the exposure to sesame allergenic foods.

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Section: Introductionmentioning

confidence: 99%

The influence of pre-treatment methods and matrix effect on sesame ( Sesamum indicum ) sandwich ELISA detection

Wang

Wang³

et al. 2021

Food and Agricultural Immunology

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“…AF169853), while Ehlert et al, 2009 [ 37 ] used a ligation-dependent probe amplification (LPA) multiplex system for the simultaneous detection of DNA from 10 different food allergens. In addition, Lopez-Calleja et al, 2017 [ 38 ] describes a multiplex ligation-dependent probe amplification (MLPA) technique for the simultaneous detection of five food allergens: sunflower, poppy, flaxseed, sesame, and soy.…”

Section: Introductionmentioning

confidence: 99%

“…No. AY677201) was the target gene of two real-time PCR with the hydrolysis/TaqMan probe method [ 38 , 44 ], obtaining a sensitivity level and a relative LOD of 4 mg/kg of pistachio in cookies and 0.1 mg/kg of pistachio nut DNA, respectively. Finally, Pis v 1 (NCBI acc.…”

Section: Introductionmentioning

confidence: 99%

“…No. DQ631675.1) was the target gene in three methods: a multiplex PCR coupled to capillary electrophoresis [ 45 ] with a relative LOD of 0.005% of pistachio in maize powder, and a SYBR-Green and locked nucleic acid (LNA) probe-based real-time PCR with an LOD of 100 mg/kg and 10 mg/kg, respectively [ 38 ]. For the considered systems, no cross-reactivity with the analysed plant or animal species was registered.…”

Section: Introductionmentioning

confidence: 99%

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Sesame, Pistachio, and Macadamia Nut: Development and Validation of New Allergenic Systems for Fast Real-Time PCR Application

Torricelli

Pierboni

Rondini

et al. 2020

Foods

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Food allergy is a worldwide health problem that concerns infants to adults. The main health risk for sensitised individuals is due to the presence of traces of allergens as the result of an accidental contamination during food processing. The labelling of allergens such as sesame, pistachio, and macadamia nut on food products is mandatory according to Regulation (EU) N. 1169/2011; therefore, the development of suitable and specific analytical methodologies is advisable. The aim of this study was to perform a multi-allergen real-time PCR system that works well in fast mode at the same annealing temperature and with the same thermal profile. The real-time PCR was developed designing new, specific, and efficient primer and probe systems for the 2S albumingene for sesame and pistachio and for the vicilin precursorgene for macadamia nut. These systems were subjected to a robust intra-laboratory qualitative validation process prior to their application, by DNA extraction and fast real-time PCR, on some real market samples to reproduce a potential allergen contamination along the food chain. The developed system results were specific and robust, with a sensible limit of detection (0.005% for sesame; 0.004% for pistachio; 0.006% for macadamia nut). The performance and the reliability of the target systems were confirmed on commercial food samples. This molecular approach could be used as a screening or as a support tool, in association with the other widespread monitoring techniques (such as ELISA).

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“…The multiplex ligation-dependent probe amplification (MLPA) (Schouten et al, 2002) as an emerging molecular diagnostic technique can detect the difference in nucleic acid sequences with a small amount of DNA and possess capability of detecting up to 50 genomic DNA sequence difference in one reaction relied on DNA denaturation, hybridization, ligation, and PCR reaction (Os and Schouten, 2011;Samelak-Czajka et al, 2017). Recently, the technology has been applied to the clinical diagnosis of diseases, such as Duchenne muscular dystrophy (Guertler et al, 2014), Gaucher disease (Schouten et al, 2002), Acute leukemia (Reyes-Núñez et al, 2017), and it also has been received wide attentions in the field of allergen detection (López-Calleja et al, 2016), genetically modified species identification (Holck et al, 2009), and herb identification (Bo et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Multiplex Ligation-Dependent Probe Amplification for Simultaneous Identification of Bungarus multicinctus and Its Common Adulterants in a Single Assay

Zhou

Nie²,

et al. 2020

Front. Pharmacol.

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Bungarus multicinctus, an important traditional Chinese medicine, possesses remarkable medicinal activities, while lots of adulterants from other species were misused as B. multicinctus for its large demand and resource starvation. In order to accurately identify B. multicinctus and its common adulterants such as Sinonatrix annularis, Xenochrophis flavipunctatus, Deinagkistrodon acutus, and Naja atra, a simultaneous identification method was designed with multiplex ligation-dependent probe amplification (MLPA) analysis. Five species-specific MLPA probe-couples for B. multicinctus and its common adulterants were designed based on the universal primer amplified COI sequences, which can specifically detect the five species with no mutual interference, and sensitivity analysis showed as less as 5% B. multicinctus or 8.75% adulterants in the mixed samples can be identified in a MLPA assay, especially, the relative quantity of the adulterants can be also inferred based on the MLPA peak area values. Moreover, the results of the present study confirmed the effectiveness of this technique in terms of simultaneous identification of B. multicinctus and its common adulterants in an assay, which has great potential for ensuring the safety of this commercially valuable snake species.

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Multiplex ligation-dependent probe amplification (MLPA) for simultaneous detection of DNA from sunflower, poppy, flaxseed, sesame and soy allergenic ingredients in commercial food products

Cited by 26 publications

References 33 publications

The influence of pre-treatment methods and matrix effect on sesame ( Sesamum indicum ) sandwich ELISA detection

The influence of pre-treatment methods and matrix effect on sesame ( Sesamum indicum ) sandwich ELISA detection

Sesame, Pistachio, and Macadamia Nut: Development and Validation of New Allergenic Systems for Fast Real-Time PCR Application

Multiplex Ligation-Dependent Probe Amplification for Simultaneous Identification of Bungarus multicinctus and Its Common Adulterants in a Single Assay

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