Multiple molecules of integration host factor (IHF) at a single DNA binding site, the bacteriophage λ cos I1 site

Integration Host Factor Assembly at the Cohesive End Site of the Bacteriophage Lambda Genome: Implications for Viral DNA Packaging and Bacterial Gene Regulation

“…DNase I and hydroxy radical footprinting studies have demonstrated that the I1 element is strongly protected by IHF, that the I2 element may be weakly protected, but that there is little to no protection at any of the other proposed elements. 33 − 35 To dissect the binding interactions more fully and to derive thermodynamic binding parameters, we utilized minimal duplex substrates to define the affinity of IHF for the putative specific elements found within cos .…”

“…Xin and Feiss identified several potential IHF binding elements within cos (Figure B), based on the H-element consensus sequence originally defined by Craig and Nash. , The putative elements possess varying degrees of sequence homology to the well-characterized H-elements but differ from the “conventional” IHF recognition sequences with respect to their spacing and orientation within cos . DNase I and hydroxy radical footprinting studies have demonstrated that the I1 element is strongly protected by IHF, that the I2 element may be weakly protected, but that there is little to no protection at any of the other proposed elements. − To dissect the binding interactions more fully and to derive thermodynamic binding parameters, we utilized minimal duplex substrates to define the affinity of IHF for the putative specific elements found within cos .…”

Integration Host Factor Assembly at the Cohesive End Site of the Bacteriophage Lambda Genome: Implications for Viral DNA Packaging and Bacterial Gene Regulation

“…DNase I and hydroxy radical footprinting studies have demonstrated that the I1 element is strongly protected by IHF, that the I2 element may be weakly protected, but that there is little to no protection at any of the other proposed elements. 33 − 35 To dissect the binding interactions more fully and to derive thermodynamic binding parameters, we utilized minimal duplex substrates to define the affinity of IHF for the putative specific elements found within cos .…”

“…Xin and Feiss identified several potential IHF binding elements within cos (Figure B), based on the H-element consensus sequence originally defined by Craig and Nash. , The putative elements possess varying degrees of sequence homology to the well-characterized H-elements but differ from the “conventional” IHF recognition sequences with respect to their spacing and orientation within cos . DNase I and hydroxy radical footprinting studies have demonstrated that the I1 element is strongly protected by IHF, that the I2 element may be weakly protected, but that there is little to no protection at any of the other proposed elements. − To dissect the binding interactions more fully and to derive thermodynamic binding parameters, we utilized minimal duplex substrates to define the affinity of IHF for the putative specific elements found within cos .…”

The λ Integrase Site-specific Recombination Pathway

“…As has not infrequently been the case, an E. coli protein discovered for its role in a phage life cycle, turned out to be an important player in the physiology of the cell. IHF is involved in regulation of gene transcription, especially σ 54 promoters (105), initiation of DNA replication (106), transposition (107), and phage packaging (108) (for reviews see references 109, 110, 111, 112, 113, 114, 115, 116).…”

Integration Host Factor Assembly at the Cohesive End Site of the Bacteriophage Lambda Genome: Implications for Viral DNA Packaging and Bacterial Gene Regulation

“…DNase I and hydroxy radical footprinting studies have demonstrated that the I1 element is strongly protected by IHF, that the I2 element may be weakly protected, but that there is little to no protection at any of the other proposed elements. 33 − 35 To dissect the binding interactions more fully and to derive thermodynamic binding parameters, we utilized minimal duplex substrates to define the affinity of IHF for the putative specific elements found within cos .…”

“…Xin and Feiss identified several potential IHF binding elements within cos (Figure B), based on the H-element consensus sequence originally defined by Craig and Nash. , The putative elements possess varying degrees of sequence homology to the well-characterized H-elements but differ from the “conventional” IHF recognition sequences with respect to their spacing and orientation within cos . DNase I and hydroxy radical footprinting studies have demonstrated that the I1 element is strongly protected by IHF, that the I2 element may be weakly protected, but that there is little to no protection at any of the other proposed elements. − To dissect the binding interactions more fully and to derive thermodynamic binding parameters, we utilized minimal duplex substrates to define the affinity of IHF for the putative specific elements found within cos .…”

The λ Integrase Site-specific Recombination Pathway

“…As has not infrequently been the case, an E. coli protein discovered for its role in a phage life cycle, turned out to be an important player in the physiology of the cell. IHF is involved in regulation of gene transcription, especially σ 54 promoters (105), initiation of DNA replication (106), transposition (107), and phage packaging (108) (for reviews see references 109, 110, 111, 112, 113, 114, 115, 116).…”

Integration Host Factor Assembly at the Cohesive End Site of the Bacteriophage Lambda Genome: Implications for Viral DNA Packaging and Bacterial Gene Regulation

“…DNase I and hydroxy radical footprinting studies have demonstrated that the I1 element is strongly protected by IHF, that the I2 element may be weakly protected, but that there is little to no protection at any of the other proposed elements. 33 − 35 To dissect the binding interactions more fully and to derive thermodynamic binding parameters, we utilized minimal duplex substrates to define the affinity of IHF for the putative specific elements found within cos .…”

“…Xin and Feiss identified several potential IHF binding elements within cos (Figure B), based on the H-element consensus sequence originally defined by Craig and Nash. , The putative elements possess varying degrees of sequence homology to the well-characterized H-elements but differ from the “conventional” IHF recognition sequences with respect to their spacing and orientation within cos . DNase I and hydroxy radical footprinting studies have demonstrated that the I1 element is strongly protected by IHF, that the I2 element may be weakly protected, but that there is little to no protection at any of the other proposed elements. − To dissect the binding interactions more fully and to derive thermodynamic binding parameters, we utilized minimal duplex substrates to define the affinity of IHF for the putative specific elements found within cos .…”

The λ Integrase Site-specific Recombination Pathway

“…As has not infrequently been the case, an E. coli protein discovered for its role in a phage life cycle, turned out to be an important player in the physiology of the cell. IHF is involved in regulation of gene transcription, especially σ 54 promoters (105), initiation of DNA replication (106), transposition (107), and phage packaging (108) (for reviews see references 109, 110, 111, 112, 113, 114, 115, 116).…”