Multiome Perturb-seq unlocks scalable discovery of integrated perturbation effects on the transcriptome and epigenome
Eli Metzner,
Kaden M. Southard,
Thomas M. Norman
Abstract:Single-cell CRISPR screens link genetic perturbations to transcriptional states, but high-throughput methods connecting these induced changes to their regulatory foundations are limited. Here we introduce Multiome Perturb-seq, extending single-cell CRISPR screens to simultaneously measure perturbation-induced changes in gene expression and chromatin accessibility. We apply Multiome Perturb-seq in a CRISPRi screen of 13 chromatin remodelers in human RPE-1 cells, achieving efficient assignment of sgRNA identitie… Show more
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