Abstract:ICPO, a herpes simplex virus immediate-early gene product, is a highly phosphorylated nuclear protein that is a potent activator of virus and host genes. Using biochemical and genetic assays employing plasmids encoding mutant forms of ICPO and a recombinant adenovirus that expresses ICPO, we provide evidence that the protein multimerizes. Some mutant forms of ICPO were transdominant and interfered with activation of a target reporter gene or with complementation of an ICPO-minus virus.
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