Multilocus Sequence Typing Tool for<i>Cyclospora cayetanensis</i>

Guo, Yaqiong; Roellig, Dawn M.; Li, Na; Tang, Kevin; Frace, Michael; Ortega, Ynés R.; Arrowood, Michael J.; Feng, Yaoyu; Qvarnström, Yvonne; Wang, Lin; Moss, Delynn M.; Zhang, Longxian; Xiao, Lihua

doi:10.3201/eid2208.150696

Cited by 37 publications

(58 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Following the generation of C. cayetanensis whole genome assemblies, several studies aimed at the development of typing tools have been published. Guo et al [32] reported the development of a multi-locus sequence typing (MLST) tool based on microsatellite and minisatellite sequences found in the C. cayetanensis genome. A total of 34 isolates with complete sequence data at five loci, formed 25 MLST types.…”

Section: Discussionmentioning

confidence: 99%

“…The authors reported several challenges such as failure of nested PCR reactions and failure of Sanger sequencing reactions presumably due to mixed C. cayetanensis populations, which may lead to unreadable sequences [32]. Another problem reported by the authors was that the geographical clustering pattern of specimens from the same country at one locus did not confirm to patterns at other loci, presumably because of genetic recombination events [32,33]. In another study, the MLST method was applied to 76 C. cayetanensis-positive samples from China.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular typing of Cyclospora cayetanensis in produce and clinical samples using targeted enrichment of complete mitochondrial genomes and next-generation sequencing

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background: Outbreaks of cyclosporiasis, a diarrheal illness caused by Cyclospora cayetanensis, have been a public health issue in the USA since the mid 1990's. In 2018, 2299 domestically acquired cases of cyclosporiasis were reported in the USA as a result of multiple large outbreaks linked to different fresh produce commodities. Outbreak investigations are hindered by the absence of standardized molecular epidemiological tools for C. cayetanensis. For other apicomplexan coccidian parasites, multicopy organellar DNA such as mitochondrial genomes have been used for detection and molecular typing. Methods: We developed a workflow to obtain complete mitochondrial genome sequences from cilantro samples and clinical samples for typing of C. cayetanensis isolates. The 6.3 kb long C. cayetanensis mitochondrial genome was amplified by PCR in four overlapping amplicons from genomic DNA extracted from cilantro, seeded with oocysts, and from stool samples positive for C. cayetanensis by diagnostic methods. DNA sequence libraries of pooled amplicons were prepared and sequenced via next-generation sequencing (NGS). Sequence reads were assembled using a custom bioinformatics pipeline. Results: This approach allowed us to sequence complete mitochondrial genomes from the samples studied. Sequence alterations, such as single nucleotide polymorphism (SNP) profiles and insertion and deletions (InDels), in mitochondrial genomes of 24 stool samples from patients with cyclosporiasis diagnosed in 2014, exhibited discriminatory power. The cluster dendrogram that was created based on distance matrices of the complete mitochondrial genome sequences, indicated distinct strain-level diversity among the 2014 C. cayetanensis outbreak isolates analyzed in this study. Conclusions: Our results suggest that genomic analyses of mitochondrial genome sequences may help to link outbreak cases to the source.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Molecular typing of Cyclospora cayetanensis in produce and clinical samples using targeted enrichment of complete mitochondrial genomes and next-generation sequencing

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Numerous genotyping methods for C. cayetanensis have been developed, and successfully used for epidemiological trace-back investigations of cyclosporiasis. Recently, a multilocus sequence typing (MLST) tool involving five microsatellite loci has been established and used for epidemiological source tracking of C. cayetanensis (Guo et al, 2016). There have many other potential uses of this MLST tool .…”

Section: Case-linking and Trackingmentioning

confidence: 99%

Advances in Cyclosporiasis Diagnosis and Therapeutic Intervention

Cui

et al. 2020

Front. Cell. Infect. Microbiol.

Self Cite

View full text Add to dashboard Cite

Cyclosporiasis is caused by the coccidian parasite Cyclospora cayetanensis and is associated with large and complex food-borne outbreaks worldwide. Associated symptoms include severe watery diarrhea, particularly in infants, and immune dysfunction. With the globalization of human food supply, the occurrence of cyclosporiasis has been increasing in both food growing and importing countries. As well as being a burden on the health of individual humans, cyclosporiasis is a global public health concern. Currently, no vaccine is available but early detection and treatment could result in a favorable clinical outcome. Clinical diagnosis is based on cardinal clinical symptoms and conventional laboratory methods, which usually involve microscopic examination of wet smears, staining tests, fluorescence microscopy, serological testing, or DNA testing for oocysts in the stool. Detection in the vehicle of infection, which can be fresh produce, water, or soil is helpful for case-linkage and source-tracking during cyclosporiasis outbreaks. Treatment with trimethoprim-sulfamethoxazole (TMP-SMX) can evidently cure C. cayetanensis infection. However, TMP-SMX is not suitable for patients having sulfonamide intolerance. In such case ciprofloxacin, although less effective than TMP-SMX, is a good option. Another drug of choice is nitazoxanide that can be used in the cases of sulfonamide intolerance and ciprofloxacin resistance. More epidemiological research investigating cyclosporiasis in humans should be conducted worldwide, to achieve a better understanding of its characteristics in this regard. It is also necessary to establish in vitro and/or in vivo protocols for cultivating C. cayetanensis, to facilitate the development of rapid, convenient, precise, and economical detection methods for diagnosis, as well as more effective tracing methods. This review focuses on the advances in clinical features, diagnosis, and therapeutic intervention of cyclosporiasis.

show abstract

“…Nuclear and mitochondrial draft genomes of C. cayetanensis are now available (Qvarnstrom et al, 2015;Guo et al, 2016;Cinar et al, 2015), leading to initial genomic investigations, including MLST analysis (Guo et al, 2016;Hofstetter et al, 2019;Li et al, 2015a). Using genome information and MLST analysis, Guo et al (2016) found variations among C. cayetanensis strains recovered from China, Nepal, Indonesia, Guatemala, Peru, Spain, and the U.S. They found that strains from China grouped well together, whereas types from the U.S. also grouped well together along with types from Peru.…”

Section: Variability Within C Cayetanensismentioning

confidence: 99%

A Review of Cyclospora cayetanensis Transport in the Environment

Onstad¹,

Miller²,

Green³

et al. 2019

Transactions of the ASABE

View full text Add to dashboard Cite

Abstract. Cyclosporiasis, a human illness marked by diarrhea, has recently emerged as a foodborne illness of concern in North America. While other reviews have focused on the epidemiology of , we focus on the environmental fate and transport of . Given the fact that cyclosporiasis is emerging in countries with advanced sanitation practices, the movement of oocysts through the environment is an important facet of the pathogen. Relatively little is known about , as it is difficult to source in developed countries, and there is no known method to culture the protozoan in the lab. This review looks at the characteristics of that allow it to survive in the environment and the effects of on humans. Keywords: Cyclospora cayetanensis, Emerging pathogen, Oocyst, Environmental fate and transport, Contamination, Protozoa.

show abstract

Multilocus Sequence Typing Tool forCyclospora cayetanensis

Cited by 37 publications

References 4 publications

Molecular typing of Cyclospora cayetanensis in produce and clinical samples using targeted enrichment of complete mitochondrial genomes and next-generation sequencing

Molecular typing of Cyclospora cayetanensis in produce and clinical samples using targeted enrichment of complete mitochondrial genomes and next-generation sequencing

Advances in Cyclosporiasis Diagnosis and Therapeutic Intervention

A Review of Cyclospora cayetanensis Transport in the Environment

Contact Info

Product

Resources

About