Multilocus enzyme electrophoresis analysis of Trypanosoma cruzi isolates from a geographically restricted endemic area for Chagas’ disease in Argentina

Diosque, Patricio; Barnabé, Christian; Padilla, Ángel M.; Marco, Jorge Diego; Cardozo, Rubén Marino; Cimino, Rubén O.; Nasser, Julio R.; Tibayrenc, Michel; Basombrío, Miguel Á.

doi:10.1016/s0020-7519(03)00139-5

Cited by 77 publications

(101 citation statements)

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“…Households harboring infected dogs or cats were observed and predicted to be at greater risk of transmission (Cohen and Gürtler, 2001;Gürtler et al, 2005), but molecular evidence of the links between bugs and host species at the household level were lacking. In a similar study in Chaco province, different lineages were found infecting dogs and humans (Diosque et al, 2003). This pattern was explained mainly by host selection of lineages (i.e.…”

Section: Discussionmentioning

confidence: 81%

“…To assess the occurrence of variation in the distribution of T. cruzi lineages among hosts within the same region we compared our results (2000)(2001)(2002)(2003)(2004)(2005)(2006)(2007) with data collected in Santiago del Estero Province in the late 1980s (De Luca D'Oro et al 1993) and in Chaco Province over 1999(Diosque et al 2003 (Table 3). Given the low numbers of humans and opossums sampled and the homogeneity of results, for these host species we pooled our data with the Chaco data and compared these with data published by De Luca D 'Oro et al (1993).…”

Section: Lineage Distribution Among Hostsmentioning

confidence: 99%

“…Natural infection by T. cruzi has been found in several local species of sylvatic mammals (Carcavallo and Martínez, 1968;Ceballos et al, 2006;Diosque et al, 2003;Yeo et al, 2005), whereas the putative sylvatic vectors have only been identified in Bolivia (Noireau et al, 2000). In the Gran Chaco, the bug Triatoma infestans is the main or single domiciliary vector; dogs, humans and chickens are usually the main blood meal sources of domestic bugs, and dogs and cats are considered the most important domestic reservoir hosts of T. cruzi (Minter, 1976;Gürtler et al, 1997Gürtler et al, , 2007a.…”

Section: Introductionmentioning

confidence: 99%

“…In Argentina, very few studies have attempted to type the local T. cruzi strains, and parasites have been characterized mainly by multilocus enzyme electrophoresis (MLEE) (Montamat et al, 1987;De Luca d'Oro et al, 1993;Diosque et al, 2003). Parasite zymodemes reported by De Luca d 'Oro et al (1993) were later analyzed by Barnabé et al (2000) and reinterpreted as belonging to TC I, TC IIb and TC IId.…”

Section: Introductionmentioning

confidence: 99%

“…Overall, T. cruzi II (TC II) was more prevalent in the domestic environment, and sylvatic mammals were found infected with both TC I and TC II (De Luca d'Oro et al, 1993). TC IIe was first reported in Argentina by Diosque et al (2003) and TC IIc by Ceballos et al (2006).…”

Section: Introductionmentioning

confidence: 99%

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Molecular epidemiology of domestic and sylvatic Trypanosoma cruzi infection in rural northwestern Argentina

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Genetic diversity of Trypanosoma cruzi populations and parasite transmission dynamics have been well documented throughout the Americas, but few studies have been conducted in the Gran Chaco ecoregion, one of the most highly endemic areas for Chagas disease, caused by T. cruzi. In this study we assessed the distribution of T. cruzi lineages (identified by PCR strategies) in Triatoma infestans, domestic dogs, cats, humans and sylvatic mammals from two neighboring rural areas with different histories of transmission and vector control in northern Argentina. Lineage II predominated among the 99 isolates characterized and lineage I among the six isolates obtained from sylvatic mammals. Trypanosoma cruzi lineage IIe predominated in domestic habitats; it was found in 87% of 54 isolates from Tr. infestans, in 82% of 33 isolates from dogs, and in the four cats found infected. Domestic and sylvatic cycles overlapped in the study area in the late 1980s, when intense domestic transmission occurred, and still overlap marginally. The introduction of T. cruzi from sylvatic into domestic habitats is likely to occur very rarely in the current epidemiological context. The household distribution of T. cruzi lineages showed that Tr. infestans, dogs and cats from a given house compound shared the same parasite lineage in most cases. Based on molecular evidence, this result lends further support to the importance of dogs and cats as domestic reservoir hosts of T. cruzi. We believe that in Argentina, this is the first time that lineage IIc has been isolated from naturally-infected domestic dogs and Tr. infestans.

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Section: Discussionmentioning

confidence: 81%