Multilocular cystic renal cell carcinoma is a subtype of clear cell renal cell carcinoma

Abstract

Multilocular cystic renal cell carcinoma is an uncommon low grade renal cell carcinoma with unique morphologic features. Its cytogenetic characteristics have not been fully investigated. Its relationship to typical clear cell renal cell carcinoma is uncertain. We evaluated 19 cases of multilocular cystic renal cell carcinoma diagnosed by strict morphologic criteria using the 2004 WHO classification system. The control group consisted of 19 low grade (Fuhrman grades 1 or 2) clear cell renal cell carcinomas. Chromosome 3p deletion status was determined by dual color interphase fluorescence in situ hybridization analysis. Chromosome 3p deletion was identified in 17 out of 19 (89%) of the clear cell renal cell carcinoma cases and 14 out of 19 (74%) of the multilocular cystic renal cell carcinoma cases, respectively. There was no difference in the status of chromosome 3p deletion between clear cell renal cell carcinoma and multilocular cystic renal cell carcinoma (P ¼ 0.40). These results support the concept that multilocular cystic renal cell carcinoma as a subtype of clear cell renal cell carcinoma.

Clear cell papillary renal cell carcinoma: differential diagnosis and extended immunohistochemical profile

“…In all, 14 clear cell papillary renal cell carcinomas were identified from 469 renal cell carcinoma resections (3%) that were performed from 2004 to 2006, indicating that this tumor is not rare. The majority of tumors reclassified as clear cell papillary renal cell carcinoma were originally diagnosed as Clear cell papillary renal cell carcinoma clear cell renal cell carcinoma (10), reinforcing the notion that there can be substantial morphologic overlap between these entities. Only a single tumor with an original diagnosis of papillary renal cell carcinoma was reclassified, and three were originally interpreted as multilocular cystic renal cell carcinomas.…”

“…16 Yet, despite the morphologic and immunohistochemical similarity of the two lesions, multilocular cystic renal cell carcinoma has been found to have deletion of regions of chromosome 3p by fluorescence in situ hybridization (FISH), comparable in frequency to clear cell renal cell carcinoma. 10 Mutations of the VHL (von Hippel-Lindau) gene have also been detected in multilocular cystic renal cell carcinoma. 17 In contrast, clear cell papillary renal cell carcinoma has been found to lack both of these abnormalities, 2,5,7,8 lending further support to the distinction between the two lesions.…”

“…10 To better understand the incidence of clear cell papillary renal cell carcinoma and to elucidate the spectrum of morphologic findings and frequency of the papillary, cystic, and solid components, we studied the light microscopic and immunohistochemical characteristics of 55 tumors from 34 patients.…”

Metanephric adenoma: the utility of immunohistochemical and cytogenetic analyses in differential diagnosis, including solid variant papillary renal cell carcinoma and epithelial-predominant nephroblastoma

“…Positivity Fluorescence in situ hybridization (FISH) analyses were performed as described previously. [13][14][15][16][17][18] Briefly, multiple 4-μm sections were obtained from formalin-fixed paraffin-embedded tissue blocks containing neoplastic tissue. A hematoxylin and eosinstained slide from each block was examined to identify areas of neoplastic tissue for FISH analysis.…”

Renal cell carcinoma with angioleiomyoma-like stroma: clinicopathological, immunohistochemical, and molecular features supporting classification as a distinct entity

“…20,21 In brief, for each slide, 100-150 nuclei from tumor tissue were scored for signals from probes under the fluorescence microscope with  1000 magnification. Non-neoplastic renal cortex was examined as an internal control.…”