Multifunctional protein-encapsulated polycaprolactone scaffolds: Fabrication and in vitro assessment for tissue engineering

Structural stability and sustained release of protein from a multilayer nanofiber/nanoparticle composite

“…All samples have minima at 209 and 222 nm indicating a high content of α-helix structure in proteins. The intensities of the two minima of released sample was smaller than those of the control, indicating a significant level (P value≤ 0.05) of decrease in the α-helix content of the released BSA compared to control, which must be attributed to mild acidic condition of chitosan nanoparticle preparation procedure [62,63]. Yang et.al.…”

Preparation, characterization and in vitro analysis of novel structured nanofibrous scaffolds for bone tissue engineering

“…The retention of osteoblastic phenotype at days 4, 8, 14 and 21 was evaluated by measuring the alkaline phosphatase (ALP) activity [17]. In brief, scaffolds were taken out and washed three times with PBS and then treated with 1 ml solution containing 1% triton and 5 mM MgCl 2 .…”

“…Mineralized matrix synthesis at days 4, 8, 14 and 21 was analyzed by the Alizarin Red staining method for calcium deposition [17]. In brief, scaffolds were fixed with 4% formaldehyde at 4°C for 30 min and then stained with 2% Alizarin Red solution for 10 min.…”

Structure–function characteristics of the biomaterials based on milk-derived proteins

“…PCL Whey protein isolate (WPI)/PCL Lactalbumin (LA)/PCL WPI-10 WPI-20 WPI-30 LA-10 LA-20 LA-30 Polycaprolactone, g 100 90 80 70 90 80 70 Whey protein isolate, g -10 20 30 ---Lactalbumin, g ----10 20 30 were non-cytotoxic and they also exhibited favorable cell adhesion and penetration characteristics [20]. The WPI exhibits an aggregated globular-like structure because of ␤-lactoglobulin and BSA that contain free thiol group.…”