Multifunctional laser speckle imaging

Du, E; Shen, Shuhao; Chong, Shau Poh; Chen, Nanguang

doi:10.1364/boe.388856

Cited by 26 publications

(16 citation statements)

References 31 publications

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“…However, conventional LSCI is based on wide-field optical imaging, which suffers from deteriorated spatial resolution and contrast when the sample is optically thick. In a previous paper, line scan laser speckle contrast imaging (LS-LSCI) was proven with good experimental results with higher spatial resolution than conventional laser speckle contrast imaging [35,36]. In this paper, we further compared the performance of spatial and temporal speckle contrast-based image acquisition approaches, and verified the flow quantification capability of our speckle imaging system, which features confocal illumination and detection.…”

Section: Introductionmentioning

confidence: 62%

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Line Scan Spatial Speckle Contrast Imaging and Its Application in Blood Flow Imaging

Shen

Qiu

et al. 2021

Applied Sciences

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Laser speckle imaging has been an indispensable tool for visualizing blood flow in biomedical applications. We proposed a novel design of the laser speckle imaging system, which combines confocal illumination and detection with various speckle analysis methods. The system can be operated by three imaging modes. One is surface illumination laser speckle contrast imaging (SI-LSCI) and the other two are line scan temporal speckle contrast imaging (LS-TSCI) and line scan spatial speckle contrast imaging (LS-SSCI). The experimental results of flow phantoms have validated the mixture model, which combines the Lorentzian and Gaussian models to describe the simultaneous existence of both Brownian motions and ordered flow. Our experimental results of in vivo chick embryos demonstrate that LS-SSCI maintains high temporal resolution and is less affected by motion artifacts. LS-SSCI can provide better image quality for in vivo imaging blood chick embryos than LS-TSCI. Furthermore, the experiential results present that LS-SSCI can detect and quantify the blood flow change during vascular clipping, and shows great potential in diagnosing vascular diseases, such as angiosclerosis, angiostenosis, or angiemphraxis.

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Section: Introductionmentioning

confidence: 62%

“…Figure 1 shows the layout of our laser speckle imaging. The details of the experimental setup are described in our previous publication [35]. The light source was a 640 nm singlefrequency laser with 25 mW.…”

Section: Methodsmentioning

confidence: 99%

Line Scan Spatial Speckle Contrast Imaging and Its Application in Blood Flow Imaging

Shen

Qiu

et al. 2021

Applied Sciences

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“…Nonetheless, the focused illumination needs to be scanned across the sample surface and the image acquisition speed usually is slowed down significantly, especially in case of point-to-point scanning. Recently, the line-scanning strategy has been adopted in fluorescence microscopy 32−34 as well as optical coherence tomography 35 to achieve the optimal compromise between the imaging speed and multiple scattering sup-pression. The combination of line-scanning image acquisition and speckle contrast, however, has yet accomplished the desired temporal resolution for dynamic flow imaging 36 .…”

Section: Introductionmentioning

confidence: 99%

Confocal laser speckle autocorrelation imaging of dynamic flow in microvasculature

Shen

Qiu

et al. 2022

OEA

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Laser speckle imaging has been widely used for in-vivo visualization of blood perfusion in biological tissues. However, existing laser speckle imaging techniques suffer from limited quantification accuracy and spatial resolution. Here we report a novel design and implementation of a powerful laser speckle imaging platform to solve the two critical limitations. The core technique of our platform is a combination of line scan confocal microscopy with laser speckle autocorrelation imaging, which is termed Line Scan Laser Speckle Autocorrelation Imaging (LS-LSAI). The technical advantages of LS-LSAI include high spatial resolution (~4.4 μm) for visualizing and quantifying blood flow in microvessels, as well as videorate imaging speed for tracing dynamic flow.

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“…OCT and photoacoustics provide three-dimensional images, however they suffer from limited field-of-view (FOV) and need a complicated experimental setup. To overcome these challenges, this study adopts laser speckle contrast imaging (LSCI) 26 – 28 and side-stream dark field (SDF) 29 microscopy to probe the spatial and temporal profile of blood flow distribution and erythrocyte velocities within individual capillaries. LSCI is noninvasive, requires a rather simple experimental setup and provides a wide FOV typically in the range of several square centimeters; however, it is a two-dimensional imaging modality.…”

Section: Introductionmentioning

confidence: 99%

Assessment of flow within developing chicken vasculature and biofabricated vascularized tissues using multimodal imaging techniques

Padmanaban

Chizari

Knop

et al. 2021

Sci Rep

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Fluid flow shear stresses are strong regulators for directing the organization of vascular networks. Knowledge of structural and flow dynamics information within complex vasculature is essential for tuning the vascular organization within engineered tissues, by manipulating flows. However, reported investigations of vascular organization and their associated flow dynamics within complex vasculature over time are limited, due to limitations in the available physiological pre-clinical models, and the optical inaccessibility and aseptic nature of these models. Here, we developed laser speckle contrast imaging (LSCI) and side-stream dark field microscopy (SDF) systems to map the vascular organization, spatio-temporal blood flow fluctuations as well as erythrocytes movements within individual blood vessels of developing chick embryo, cultured within an artificial eggshell system. By combining imaging data and computational simulations, we estimated fluid flow shear stresses within multiscale vasculature of varying complexity. Furthermore, we demonstrated the LSCI compatibility with bioengineered perfusable muscle tissue constructs, fabricated via molding techniques. The presented application of LSCI and SDF on perfusable tissues enables us to study the flow perfusion effects in a non-invasive fashion. The gained knowledge can help to use fluid perfusion in order to tune and control multiscale vascular organization within engineered tissues.

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Multifunctional laser speckle imaging

Cited by 26 publications

References 31 publications

Line Scan Spatial Speckle Contrast Imaging and Its Application in Blood Flow Imaging

Line Scan Spatial Speckle Contrast Imaging and Its Application in Blood Flow Imaging

Confocal laser speckle autocorrelation imaging of dynamic flow in microvasculature

Assessment of flow within developing chicken vasculature and biofabricated vascularized tissues using multimodal imaging techniques

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