Multifaceted roles of the egg perivitelline layer in avian reproduction: Functional insights from the proteomes of chicken egg inner and outer sublayers

Brégeon, Mégane; Tomas, Daniel; Bernay, Benoı̂t; Zatylny-Gaudin, Céline; Georgeault, Sonia; Labas, Valérie; Réhault-Godbert, Sophie; Guyot, Nicolas

doi:10.1016/j.jprot.2022.104489

Cited by 9 publications

(6 citation statements)

References 78 publications

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“…Another category of known PCR inhibitors are proteases (Schrader et al., 2012), which are also present in egg yolk (Shbailat et al., 2016). A total of 412 proteins, including glycoproteins, have also been identified in the chicken perivitelline layer (Brégeon et al., 2022), which may also act as inhibitors. Samples containing perivitelline layer had the worst rates of microsatellite amplification in this study, which may be a consequence of the large number of proteins present in those samples.…”

Section: Discussionmentioning

confidence: 99%

Including the invisible fraction in whole population studies: A guide to the genetic sampling of unhatched bird eggs

Morland,

Patel,

Santure

et al. 2023

Methods Ecol Evol

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Early embryo mortality has recently been proven to be a significant component of avian reproductive failure. Due to the difficulty in distinguishing eggs, which have suffered early embryo mortality from unfertilised eggs, this cause of reproductive failure has historically been underestimated and overlooked. We describe methods for recognising and collecting early failed, unhatched eggs from wild bird populations, identifying and isolating embryonic material in unhatched eggs, and efficiently extracting DNA from those samples. We test these methods on unhatched hihi (Notiomystis cincta) eggs collected from the field, which have undergone postmortem incubation. We obtained DNA yields from early‐stage embryos that are sufficient for a wide range of molecular techniques, including microsatellite genotyping for parentage analysis and sex‐typing. The type of tissue sample taken from the egg affected downstream DNA yields and microsatellite amplification rates. Species‐specific microsatellite markers had higher amplification success rates than cross‐species markers. We make key recommendations for each stage of the sampling and extraction process and suggest potential protocol improvements and modifications. Genetic and possibly genomic analysis of embryos that die early in development has the potential to advance many fields. The methods described here will allow a more in‐depth exploration of the previously overlooked causes of early embryo mortality in wild populations of birds, including threatened species.

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Section: Discussionmentioning

confidence: 99%

Including the invisible fraction in whole population studies: A guide to the genetic sampling of unhatched bird eggs

Morland,

Patel,

Santure

et al. 2023

Methods Ecol Evol

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“…Further studies on the impact of extended storage (egg white physicochemistry, ultrastructure of the vitelline membrane that supports the embryo) may be useful to identify the determinant parameters that alter embryo survival and restarting. We believe that the quality of the vitelline membrane is crucial in the early stages of incubation as its inner layer contains many proteins that are assumed to support the development of the embryo and the expansion of the yolk sac during embryogenesis ( Brégeon et al, 2022 ). Alteration of the perivitelline membrane is supposed to be a key determinant that can explain very early mortality.…”

Section: Discussionmentioning

confidence: 99%

How Egg Storage Duration Prior to Incubation Impairs Egg Quality and Chicken Embryonic Development: Contribution of Imaging Technologies

et al. 2022

Self Cite

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Storing fertilised eggs prior to incubation is a frequent practice in commercial hatcheries to coordinate activities and synchronise hatchings. However, the conditions used to store eggs can have major impacts on egg quality and the subsequent viability of chicken embryos. While storage temperatures of 16–18°C are classically used in hatcheries, the duration of storage varies from three to more than 10 days. We explored the effect of storage duration (zero, three or 10 days; D0, D3 and D10, respectively) at 16°C, 80% relative humidity (RH) on egg quality (Broiler, Ross 308), using computed tomography (CT) and classical measurements (egg weight, eggshell strength, egg white pH, Haugh units, yolk index and colour). The results revealed that a storage duration of up to 10 days negatively affected some egg quality traits (yolk index and volume, air chamber volume and egg white pH). Eggs stored for three or 10 days were further incubated for 11, 13 or 15 days (37.8°C, 55% RH). Eggs were analysed by magnetic resonance imaging (MRI) and CT to assess the development of the embryo and internal egg changes occurring during incubation. First, data showed that the fertility and sex ratio of eggs were not affected by storage duration. However, the mortality of viable eggs was increased in the D10 group compared to the D3 group. Results of non-invasive imaging technologies revealed that the storage of eggs for 10 days impaired embryo growth as early as 11 days of incubation (decrease in brain and embryo volumes). Collectively, these data provide new evidence that the duration of egg storage negatively affects embryonic growth. They further corroborate that this parameter is likely to be crucial to synchronising embryonic stages and maybe reducing the hatching window, hence limiting the time spent by newborn chicks in hatchers. In addition, our results highlight that CT and MRI imaging technologies are useful non-invasive tools to evaluate egg quality prior to incubation and the impact of storage (or incubation) practices on developmental growth of the embryo.

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“…Another category of known PCR inhibitors are proteases (Schrader et al, 2012), which are also present in egg yolk (Shbailat et al, 2016). A total of 412 proteins, including glycoproteins, have also been identified in the chicken perivitelline layer (Brégeon et al, 2022), which may also act as inhibitors.…”

Section: Iii) Parentage Analysis and Sex Typingmentioning

confidence: 99%

Including the Invisible Fraction in Whole Population Studies: a Guide to the Genetic Sampling of Unhatched Bird Eggs

Morland

Santure

Patel

et al. 2023

Preprint

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1. Early embryo mortality has recently been proven to be a significant component of avian reproductive failure. Due to the difficulty in distinguishing eggs which have suffered early embryo mortality from unfertilised eggs, this cause of reproductive failure has historically been underestimated and overlooked. 2. We describe methods for recognising and collecting undeveloped, unhatched eggs from wild bird populations, identifying and isolating embryonic material in unhatched eggs, and efficiently extracting DNA from those samples. We test these methods on unhatched eggs collected from the field which have undergone post-mortem incubation. 3. We obtained DNA yields from early-stage embryos that are sufficient for a wide range of molecular techniques, including microsatellite genotyping for parentage analysis and sex-typing. The type of tissue sample taken from the egg affected downstream DNA yields and microsatellite amplification rates. Species-specific microsatellite markers had higher amplification success rates than cross-species markers. We make key recommendations for each stage of the sampling and extraction process and suggest potential protocol improvements and modifications. 4. Genetic and possibly genomic analysis of embryos that die early in development has the potential to advance many fields. The methods described here will allow a more in-depth exploration of the previously overlooked causes of early embryo mortality in wild populations of birds, including threatened species.

show abstract

Multifaceted roles of the egg perivitelline layer in avian reproduction: Functional insights from the proteomes of chicken egg inner and outer sublayers

Cited by 9 publications

References 78 publications

Including the invisible fraction in whole population studies: A guide to the genetic sampling of unhatched bird eggs

Including the invisible fraction in whole population studies: A guide to the genetic sampling of unhatched bird eggs

How Egg Storage Duration Prior to Incubation Impairs Egg Quality and Chicken Embryonic Development: Contribution of Imaging Technologies

Including the Invisible Fraction in Whole Population Studies: a Guide to the Genetic Sampling of Unhatched Bird Eggs

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