Multidisciplinary integrated characterization of a native Chlorella-like microalgal strain isolated from a municipal landfill leachate

Evaluation of Nutraceutical Properties of Eleven Microalgal Strains Isolated from Different Freshwater Aquatic Environments: Perspectives for Their Application as Nutraceuticals

“…Four of these strains were taxonomically related to Chlorella sorokiniana [ 32 ], and were collected from Lake Massaciuccoli (namely strains Idr, CL_Sc, CL_Ch, and FB). The strain SEC_Li_ChL_1 was isolated from an artificial lake in Rosignano Marittimo (LI) and was taxonomically identified as part of the Chlorella–Micractinium clade [ 33 ]. These five strains were kindly provided from the collection of the Dept.…”

“…Six of the eleven microalgal strains used in this work were not previously characterized or published (F1, F2, F3, F4, C2, and R1); accordingly, a molecular characterization based on the sequencing of the 18S and/or the ITS rRNA sequences was performed, as described in Ciurli et al [ 33 ]. Briefly, total genomic DNA was extracted following the protocol described by Saba et al [ 35 ], starting from a fresh pellet obtained by centrifugation of 2 mL of each liquid monostrain microalgal culture (5′ at 14,000× g —Eppendorf 5415R).…”

“…Briefly, total genomic DNA was extracted following the protocol described by Saba et al [ 35 ], starting from a fresh pellet obtained by centrifugation of 2 mL of each liquid monostrain microalgal culture (5′ at 14,000× g —Eppendorf 5415R). The 18S rRNA and the fragment comprising the final portion of 18S, the complete ITS1-5.8S-ITS2, and the initial portion of 28S were amplified as described in Ciurli et al [ 33 ], using a C1000™ Thermal Cycler (Bio-Rad, CA, USA). Amplicons obtained from F2 and F3 and C2 microalgal cultures were purified by ethanol/EDTA/Na–acetate precipitation, according to Ciurli et al [ 33 ], and they were sequenced by the company “BMR Genomics” (Padova, Italy).…”

First application of different <em>Chlorella</em> sp. microalgal strains for the treatment of vegetation waters derived from unconventional oil extractions enriched with citrus byproducts

“…In both the experiments here described, the microalgae were exposed to light for the whole experimental duration (16/08 h day-night cycle with PPFD of 120 mmol photons m-1 s-1). Anyway, if we consider that the vegetation water has a purple dark color at all the tested concentrations, we are not completely aware by the possibility that our strains, might have performed heterotrophic metabolism as well, since it is already documented that Chlorella-like strains from environmental origin are able to growth and survive in dark conditions [17].…”

“…The five microalgal strains selected for this experiment were of environmental origin. Strain SEC_Li_ChL_1 [17] was sampled in an artificial lake at the "Rosignano Energia Ambiente. S.p.A." location (now called "Scapigliato Energia s.r.l.…”