Multidimensional liquid-phase separations combining both chromatography and electrophoresis – A review

Ranjbar, Leila; Foley, Joe P.; Breadmore, Michael C.

doi:10.1016/j.aca.2016.10.025

Cited by 34 publications

(23 citation statements)

References 108 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When these 2D systems are on‐line coupled with MS or tandem MS detection, then supra‐powerful multidimensional systems are obtained capable to identify hundreds up to few thousands of peptides/proteins in the above complex biomatrices . The fact that 2D and multidimensional CE separations represent a hot topic in separation science is reflected by several recent reviews describing current state in this rapidly developing area . In this section, only few examples will be presented.…”

Section: Separations By the Particular Ce And Cec Methodsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

View full text Add to dashboard Cite

The review brings a comprehensive overview of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, microscale isolation, purification, and physicochemical and biochemical characterization of peptides in the years 2015, 2016, and ca. up to the middle of 2017. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis (sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, and detection) are described. New developments in particular CE and CEC methods are presented and several types of their applications to peptide analysis are reported: qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC methods to provide important physicochemical characteristics of peptides are demonstrated.

show abstract

Section: Separations By the Particular Ce And Cec Methodsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

View full text Add to dashboard Cite

show abstract

“…The mass spectrometer has been considerably improved in the past decade with a dramatic increase in acquisition speed and mass accuracy as well as the introduction of new fragmentation techniques [14][15][16][17]. Regarding LC, various MD separation approaches have been developed to decrease the complexity of samples, by which, peptides are consecutively separated by two or more independent separation mechanisms [6,18,19]. MD-LC systems can be carried out in an online or offline manner.…”

Section: Introductionmentioning

confidence: 99%

Review of Three-Dimensional Liquid Chromatography Platforms for Bottom-Up Proteomics

Duong

Park

Lee

2020

IJMS

View full text Add to dashboard Cite

Proteomics is a large-scale study of proteins, aiming at the description and characterization of all expressed proteins in biological systems. The expressed proteins are typically highly complex and large in abundance range. To fulfill high accuracy and sensitivity of proteome analysis, the hybrid platforms of multidimensional (MD) separations and mass spectrometry have provided the most powerful solution. Multidimensional separations provide enhanced peak capacity and reduce sample complexity, which enables mass spectrometry to analyze more proteins with high sensitivity. Although two-dimensional (2D) separations have been widely used since the early period of proteomics, three-dimensional (3D) separation was barely used by low reproducibility of separation, increased analysis time in mass spectrometry. With developments of novel microscale techniques such as nano-UPLC and improvements of mass spectrometry, the 3D separation becomes a reliable and practical selection. This review summarizes existing offline and online 3D-LC platforms developed for proteomics and their applications. In detail, setups and implementation of those systems as well as their advances are outlined. The performance of those platforms is also discussed and compared with the state-of-the-art 2D-LC. In addition, we provide some perspectives on the future developments and applications of 3D-LC in proteomics.

show abstract

“…If (i) medium electrophoretic mobility cations have a lower normalized value than -0.83, the extract is non-cytoxic. If medium (6), G. macrorrhizum (7), M. officinalis (8), A. archangelica aerial part (9), A. archangelica root (10), T. vulgaris (11), H. officinalis (12), N. cataria (13), E. purpurea (14), S. officinalis (15) Multimensional scaling of plant extracts electrophoretic mobility cations have a higher normalized value than -0.83, the extract is cytotoxic with a probability of 75%. If (ii) the extract in the second level has the normalized value of low electrophoretic mobility cations lower than -0.84, the extracts are non-cytotoxic.…”

Section: Data Mining and Chemometricsmentioning

confidence: 99%

“…Separation methods are superior over standard electrochemical, or spectrophotometric methods, since the separation step is combined with spectrometric, electrochemical or other physicochemical detection methods. Separation methods can clarify multiple factors, or peaks in a single run, therefore such means can be treated as multidimensional information providing methods [6]. Among them capillary electrophoresis, gas chromatography and liquid chromatography are dominating in phytochemical investigations [7,8].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic attributes of antiviral plants rich in polyphenolic and volatile compounds

Drevinskas¹,

Mickienė²,

Maruška³

et al. 2018

chemija

View full text Add to dashboard Cite

The application of natural products for treatment and prevention of various diseases is becoming more important. Not only antibacterial natural preparations are being developed, but also antiviral natural products are investigated. There are two main problems in such investigations: (i) special chemical analysis and data analysis methods have to be used, (ii) not only antiviral effect has to be taken into account, but also cytotoxic and other unwanted effects. In this work, a combination of separation methods (capillary electrophoresis and gas chromatography), spectrophotometric methods, data mining/data analysis methods (hierarchical clusterization, multidimensional scaling and classification and regression tree induction) for clarifying chemical attributes in cytotoxic plant extracts was applied. The selected plants belonged to families: (i) Lamiaceae, (ii) Asteraceae and (iii) Fabaceae. Most cytotoxic were Agastache foeniculum Pursh. Kuntze. and Salvia officinalis L. extracts. Least cytotoxic were Perilla frutescens L. and Satureja Montana L. extracts. The tendency of cytotoxic extracts having abundant concentrations of medium and low electrophoretic mobility cations was clarified.

show abstract

Multidimensional liquid-phase separations combining both chromatography and electrophoresis – A review

Cited by 34 publications

References 108 publications

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Review of Three-Dimensional Liquid Chromatography Platforms for Bottom-Up Proteomics

Cytotoxic attributes of antiviral plants rich in polyphenolic and volatile compounds

Contact Info

Product

Resources

About