considering to detect physiologically associated molecules in biological species currently. [4,5] They previously focused on the detection of some molecules like glucose, hydrogen peroxide (H 2 O 2 ), uric acid, and so on, which play pivotal roles in the regulation of human metabolism. [6][7][8][9] Recently, widely presented dopamine (DA), as one important member of the neurotransmitters in human central nervous, renal and hormonal systems, plays a crucial role in the regulation of human bodily functions and metabolism. [10] Fluctuations in DA levels can demonstrate certain early biochemical and pharmacological signals of critical diseases, such as Parkinsonism, schizophrenia, hyperactive disorder, and so on. [11,12] Hence, the detection of DA levels has been another main issue in the medical field.With the rapid development in modern science and technology, various innovative methods have been applied to the detection of biomolecules, especially for DA. There are rising some reliable methods for the detection of DA, including surface plasmon resonance, high-performance liquid chromatography (HPLC), fluorescence, gas chromatography-mass spectrometry, and electrochemistry. [13][14][15][16] Among these techniques, electrochemical detection has attracted widespread interest, based on its high sensitivity, briefness, and low cost. Most importantly, with abundant progress in stretchable substrates, reusable electrodes, and novel materials, electrochemical detection has endued with unique advantageous properties such as outstanding flexibility, reutilizing, and compatibility in a wide range of molecule detection. [17,18] But there are still some disadvantages unresolved like long-detecting time procedures, complicated analytic methods, and harsh-detecting conditions. Especially for DA detection, DA is a kind of biomolecule with high electrochemical activity and the bare electrode is hardly used in its detection with poor sensitivity and reproducibility.Up to now, various materials have been taken into the construct of electrochemical sensors, such as metal-organic framework hybrid material, noble metals nanoparticles, black phosphorus-based materials, semiconductors, metal oxides or sulfides, polymers, and carbon-based materials. [19][20][21][22][23][24][25] Among them, noble metal nanoparticles, particularly gold nanoparticles (AuNPs), have attracted considerable attention due to their unusual structures and distinctive properties including excellent conductivity, well mechanical and thermal properties. [26,27] Dopamine (DA) plays a crucial role in the regulation of human bodily functions and metabolism. It is necessary to detect and then to regulate DA levels for health maintenance. Electrochemical sensors have intrinsic advantages in DA determination, especially for metal nanoparticles-based sensors with excellent physicochemical properties and efficient activity. In this article, AuNPs located on functional nanographene oxide (NGO) are fabricated for DA detection. As the facial platform, NGO has large surface areas...