Multi wall carbon nanotubes induce oxidative stress and cytotoxicity in human embryonic kidney (HEK293) cells

Anreddy, Rama Narsimha Reddy; Reddy, Y. V. R.; Krishna, D. R.; Himabindu, V.

doi:10.1016/j.tox.2010.03.017

Cited by 141 publications

(90 citation statements)

References 37 publications

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“…[28][29][30][31][32][33][34] Hyun-Jeong Eom and Jinhee Choi's study showed that CeO 2 nanoparticles were associated with oxidative damage to human bronchial epithelial cells (Beas-2B cells), via extracellular signal-regulating kinase (ERK), p38, and c-Jun N-terminal kinase (JNK) signaling pathways, along with nuclear factor-kappaB (NF-κB) and nuclear factor-E 2 -related factor-2 (Nrf-2) signaling. 35 Eun-Jung Park and Kwangsik Park indicated that oxidative stress, induced by silica nanoparticles in vivo and in vitro, might have been involved in proinflammatory responses.…”

Section: Discussionmentioning

confidence: 99%

Intraperitoneal injection of magnetic Fe3O4-nanoparticle induces hepatic and renal tissue injury via oxidative stress in mice

Ma¹,

Luo²,

Chen³

et al. 2012

IJN

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Because of its unique magnetic properties, the iron oxide (Fe 3 O 4 ) nanoparticle has been widely exploited and its application in various fields has promised immense benefits. However, doubts exist over the use of Fe 3 O 4 -nanoparticles in human beings. Thus, the aim of the current study was to find out the potential safety range of medical use. Twenty-five Kunming mice were exposed to Fe 3 O 4 -nanoparticles via intraperitoneal injection daily for 1 week at doses of 0, 5, 10, 20, and 40 mg/kg. Hepatic and renal tissues were sliced for physiological observation. Injuries were observed in the high-dose groups (20 and 40 mg/kg) compared with the control group (0 mg/kg). Biomarkers of reactive oxygen species, glutathione, malondialdehyde, DNAprotein crosslinks, and 8-hydroxy-2′-deoxyguanosine in the hepatic and renal tissues were detected. Injury to tissues and oxidative damage to cells at the molecular level was found. The safest dose recommended from the results of this study is 5 mg/kg, as we believe this to be an upper limit balancing the benefits and risks for sub-long-term exposure.

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Section: Discussionmentioning

confidence: 99%

Intraperitoneal injection of magnetic Fe3O4-nanoparticle induces hepatic and renal tissue injury via oxidative stress in mice

Ma¹,

Luo²,

Chen³

et al. 2012

IJN

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“…In vitro studies reported various toxicological effects of MW-CNTs including oxidative stress, DNA damage and apoptosis in mammalian cells lines. Other effects include VE-cadherin distribution and actin filament integrity in human aortic endothelial cells [114][115][116][117][118].…”

Section: Effects On Cell Linesmentioning

confidence: 99%

In vitro and in vivo toxicity assessment of nanoparticles

2017

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Nanotechnology has revolutionized gene therapy, diagnostics and environmental remediation. Their bulk production, uses and disposal have posed threat to the environment. With the appearance of these nanoparticles in the environment, their toxicity assessment is an immediate concern. This review is an attempt to summarize the major techniques used in cytotoxity determination. The review also presents a detailed and elaborative discussion on the toxicity imposed by different types of nanoparticles including carbon nanotubes, gold nanoparticles, silver nanoparticles, quantum dots, fullerenes, aluminium nanoparticles, zinc nanoparticles, iron nanoparticles, titanium nanoparticles and silica nanoparticles. It discusses the in vitro and in vivo toxological effects of nanoparticles on bacteria, microalgae, zebrafish, crustacean, fish, rat, mouse, pig, guinea pig, human cell lines and human. It also discusses toxological effects on organs such as liver, kidney, spleen, sperm, neural tissues, liver lysosomes, spleen macrophages, glioblastoma cells, hematoma cells and various mammalian cell lines. It provides information about the effects of nanoparticles on the gene-expression, growth and reproduction of the organisms.

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“…Alterations in GSH homeostasis can be considered as an indication of functional-damage to the cells [16]. As shown in Table 2 sulfadiazine at dose 10 mg, 30 mg and 70 mg decreased GSH levels in the cells in a concentration dependent manner.…”

Section: Discussionmentioning

confidence: 95%

“…We suspected that the observed increased concentration of lipid peroxides, along with decreased GSH was capable of inducing some injuries such as apoptosis visible at the cellular level. Concomitant cellular oxidative stress was revealed by reduced GSH levels, and amplified lipid peroxidation [16].…”

Section: Discussionmentioning

confidence: 99%

Histopathological Evaluation of Dose Dependent Sulfadiazine-Associated Nephrotoxicity and Alteration on Oxidative Stress in Chicken Embryos

Sayrafi

Golshahi

Araghi

et al. 2016

Zahedan J Res Med Sci

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Background: Numerous epidemiological and experimental researches indicate that in utero exposure to some environmental chemicals and prescribed drugs during pregnancy can mediate various embryonic abnormalities and complications via reactive oxygen species (ROS) generation, which damages cellular macromolecules. Objectives: The aim of the present study was to evaluate the sulfonamide-associated nephrotoxicity with possible underlying mechanisms in chicken embryo. Materials and Methods:In this experimental study, one hundred fertile eggs were obtained and divided into five groups: 1) control group (without injection), 2) group injected with 2 mg sulfadiazine, 3) group injected with 10 mg sulfadiazine, 4) group injected with 30 mg sulfadiazine and 5) group injected with 70 mg sulfadiazine. After hatching, the renal tissue from the newly hatched chick was harvested for histopathologic investigation and also measurement of oxidative stress parameters [the ferric reducing capacity assay, the glutathione content (GSH) and the situation of lipid peroxidation (LPO)] by spectrophotometer. Results: Histologic examination of the renal tissue revealed that sulfadiazine induces hydropic degeneration, tubular necrosis, glomerular and tubular atrophy, formation of hyaline cast, congestion, hemorrhage, interstitial nephritis and fibrosis. Conclusions: Result showed the dose-dependent administration of sulfadiazine significantly altered the histopathologic structure of renal tissues of chickens. Furthermore, the major histopathologic events in the course of sulfadiazine cytotoxicity are renal tubule epithelial cell necrosis, interstitial nephritis and fibrosis, formation of hyaline cast and congestion and hemorrhage, although sulfadiazine at dose 30 mg and 70 mg caused perturbation in antioxidant defense system by marked increase in LPO, and decrease in GSH.

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Multi wall carbon nanotubes induce oxidative stress and cytotoxicity in human embryonic kidney (HEK293) cells

Cited by 141 publications

References 37 publications

Intraperitoneal injection of magnetic Fe3O4-nanoparticle induces hepatic and renal tissue injury via oxidative stress in mice

Intraperitoneal injection of magnetic Fe3O4-nanoparticle induces hepatic and renal tissue injury via oxidative stress in mice

In vitro and in vivo toxicity assessment of nanoparticles

Histopathological Evaluation of Dose Dependent Sulfadiazine-Associated Nephrotoxicity and Alteration on Oxidative Stress in Chicken Embryos

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