Multi-step thermally induced transitions of β-lactoglobulin – An in situ spectroscopy approach

Abstract

An in-situ approach based in multiple spectroscopic techniques and benchmarked with DSC was used to characterise b-Lg thermally-induced transitions. The methodology applied overcomes previously reported limitations by ensuring similar experimental conditions in different determinations, nonaggregation conditions and allowing differentiation between fluorescent variations due to collisional quenching and structural modifications. These experimental improvements along with the correlation of complementary data f…