Multi-species biofilms of environmental microbiota isolated from fruit packing facilities promoted tolerance of Listeria monocytogenes to benzalkonium chloride

Rolon, M. Laura; Voloshchuk, Olena; Bartlett, Katelyn V.; LaBorde, Luke F.; Kovac, Jasna

doi:10.1016/j.bioflm.2024.100177

Cited by 5 publications

(4 citation statements)

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“…Different studies hypothesized that (multispecies) biofilms support Listeria persistence in food processing environments, as the biofilm matrix protects Listeria from disinfectants ( Van der Veen and Abee, 2011 ; Oxaran et al, 2018 ; Rolon et al, 2024b ). In this study, we detected L. innocua on two biofilm harboring sites, located at conveyor belt guide rollers with Exiguobacterium present in abundance.…”

Section: Discussionmentioning

confidence: 99%

“…For example, Pseudomonas , Acinetobacter , or Janthinobacterium were reported to be indicative for Listeria presence in a meat processing environment ( Zwirzitz et al, 2021 ). Also, Pseudomonas , Stenotrophomonas , and Microbacterium were associated with Listeria presence in tree fruit packing facilities ( Rolon et al, 2024b ), and Carnobacterium , Psychrobacter , and Pseudomonas were more abundant in samples positive for Listeria in distribution centers for fresh produce ( Townsend et al, 2023 ). We also detected significant differences in the community structure between Listeria -positive and Listeria -negative samples.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, there are extensive variations in the biofilm-forming ability of different bacterial species and strains (e.g., Wagner et al, 2021 ; Di Ciccio et al, 2022 ). Multispecies biofilm models showed that L. monocytogenes is able to colonize a monospecies Pseudomonas biofilm ( Rodríguez-López et al, 2022 ; Sterniša et al, 2023 ) or even a multi-taxa biofilm ( Rolon et al, 2024b ). These studies showed that the bacterial interactions in multi-species biofilms are complex and that L. monocytogenes within biofilms is protected from environmental stressors, e.g., from antimicrobials.…”

Section: Introductionmentioning

confidence: 99%

“…The resident microbiota may interact competitive or cooperative with other bacteria including food pathogens ( De Filippis et al, 2021 ; Fagerlund et al, 2021 ; Tadielo et al, 2023 ; Xu et al, 2023 ). Diverse bacterial taxa isolated from food processing environments were shown to influence the survival and growth of Listeria under laboratory conditions ( Sinclair et al, 2022 ; Lake et al, 2023 ; Rolon et al, 2024a , b ). Positive and negative associations of members of microbial communities with L. monocytogenes and other Listeria spp.…”

Section: Introductionmentioning

confidence: 99%

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Exploring the occurrence of Listeria in biofilms and deciphering the bacterial community in a frozen vegetable producing environment

Pracser,

Voglauer,

Thalguter

et al. 2024

Front. Microbiol.

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The establishment of Listeria (L.) monocytogenes within food processing environments constitutes a significant public health concern. This versatile bacterium demonstrates an exceptional capacity to endure challenging environmental conditions in the food processing environment, where contamination of food products regularly occurs. The diverse repertoire of stress resistance genes, the potential to colonize biofilms, and the support of a co-existing microbiota have been proposed as root causes for the survival of L. monocytogenes in food processing environments. In this study, 71 sites were sampled after cleaning and disinfection in a European frozen vegetable processing facility, where L. monocytogenes in-house clones persisted for years. L. monocytogenes and L. innocua were detected by a culture-dependent method at 14 sampling sites, primarily on conveyor belts and associated parts. The presence of biofilms, as determined by the quantification of bacterial load and the analysis of extracellular matrix components (carbohydrates, proteins, extracellular DNA) was confirmed at nine sites (12.7%). In two cases, L. innocua was detected in a biofilm. Furthermore, we explored the resident microbial community in the processing environment and on biofilm-positive sites, as well as the co-occurrence of bacterial taxa with Listeria by 16S rRNA gene sequencing. Pseudomonas, Acinetobacter, and Exiguobacterium dominated the microbial community of the processing environment. Using differential abundance analysis, amplicon sequence variants (ASVs) assigned to Enterobacterales (Enterobacter, Serratia, unclassified Enterobacteriaceae) and Carnobacterium were found to be significantly higher abundant in Listeria-positive samples. Several Pseudomonas ASVs were less abundant in Listeria-positive compared to Listeria-negative samples. Acinetobacter, Pseudomonas, Janthinobacterium, Brevundimonas, and Exiguobacterium were key players in the microbial community in biofilms, and Exiguobacterium and Janthinobacterium were more relatively abundant in biofilms. Further, the microbial composition varied between the different areas and the surface materials.

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Section: Discussionmentioning

confidence: 99%