Multi-response optimization of oil extraction from pine nut (Pinus pinea L.) by response surface methodology: Extraction efficiency, physicochemical properties and antioxidant activity

This study evaluates the antioxidant activity of cannabidiol (CBD), added to model systems of refined olive (ROO) and sunflower (SO) oils, by measuring the peroxide value, oxidative stability index (OSI), electron spin resonance (ESR) forced oxidation, and DPPH• assays. Free acidity, a parameter of hydrolytic rancidity, was also examined. CBD was compared using the same analytical scheme with α-tocopherol. CBD, compared to α-tocopherol, showed a higher scavenging capacity, measured by DPPH• assay, but not better oxidative stability (OSI) of the oily systems considered. In particular, α-tocopherol (0.5%) showed an antioxidant activity only in SO, registered by an increase of more than 30% of the OSI (from 4.15 ± 0.07 to 6.28 ± 0.11 h). By ESR-forced oxidation assay, the concentration of free radicals (μM) in ROO decreased from 83.33 ± 4.56 to 11.23 ± 0.28 and in SO from 19.21 ± 1.39 to 6.90 ± 0.53 by adding 0.5% α-tocopherol. On the contrary, the addition of 0.5% CBD caused a worsening of the oxidative stability of ROO (from 23.58 ± 0.32 to 17.28 ± 0.18 h) and SO (from 4.93 ± 0.04 to 3.98 ± 0.04 h). Furthermore, 0.5% of CBD did not lower dramatically the concentration of free radicals (μM) as for α-tocopherol, which passed from 76.94 ± 9.04 to 72.25 ± 4.13 in ROO and from 17.91 ± 0.95 to 16.84 ± 0.25 in SO.

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“…The lack of a clear and definite correlation between these two assays for some oily matrices has also been reported in previous studies [30,31].…”

Section: Discussionsupporting

confidence: 74%

Preliminary Study: Comparison of Antioxidant Activity of Cannabidiol (CBD) and α-Tocopherol Added to Refined Olive and Sunflower Oils

2019

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“…Forest tree cones have been mostly used for seed extraction for the production of forestry propagation material. The edible seeds extracted from stone pine cones (Pinus pinea L.) are one of the most important tree nuts widely planted throughout the Mediterranean region (Kemerli-Kalbaran and Ozdemir, 2019). The residual empty (or as referred to later: opened) cones, generated in several dozen or even as much as several hundred tonnes (Aniszewska and Bereza, 2014), are usually burned in an uncompressed state or can be converted to briquettes (Gendek et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant properties assessment of the cones of conifers through the combined evaluation of multiple antioxidant assays

Hofmann

Visi-Rajczi

Albert

2020

Industrial Crops and Products

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Wood logging generates considerable amounts of waste biomass (e.g. cones, bark, leaves, and roots), worthwile to use, as a potential source of antioxidants. Polyphenols represent an important type of non-enzymatic antioxidants in plants. Current data in scientific literature regarding the antioxidant content of coniferous cones and the antioxidant concentrations at different ripening stages is limited and have not been investigated in detail yet. Our investigation aimed to implement an ultrasonic extraction method for the assessment and comparison of the antioxidant polyphenol content of conifer cones: six arbitrarily selected taxa (Cedrus atlantica, Larix decidua, Picea abies, Pinus nigra, Pseudotsuga menziesii, Tsuga canadensis) commonly found in Hungary were investigated in the present study, which provides methodology for future investigations. The comparison of green, mature, and opened cones were carried out for each taxon. Folin-Ciocâlteu total phenol content, ferric reducing ability of plasma (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays were used to assess the antioxidant properties. The 30 min ultrasonic extraction using acetone:water 80:20 v/v solution resulted in the optimum yield on total polyphenols, FRAP, and DPPH. Best values were found for green cones, followed by mature, and opened cones for each species. Overall antioxidant power was determined by a scoring system that combined the three assay results. Taxa with the highest scores were Picea abies and Tsuga Canadensis, which contained high amounts of antioxidants in both green and mature cones and, surprisingly, also in opened cones (P. abies). Results provide a basis for future investigation and comparison involving a large number of taxa.

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“…However, the temperature-time interaction affected negatively to the extraction of alpha-tocopherol. In this sense, Kemerli-Kalbaran et al (Kemerli-Kalbaran & Ozdemir, 2019) reported that although the extraction efficiency of antioxidants increased by increasing the temperature and time, its interaction had a negative impact on antioxidant activity in pine nuts. This may be caused by oxidation of antioxidant compounds such as tocopherols when high temperatures were applied for prolonged times.…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

Optimization and validation of a simplified methodology for simultaneous extraction of fatty acids and tocopherol homologues in peanuts

Polo¹,

Sanahuja²,

Moya³

et al. 2022

Journal of Food Composition and Analysis

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Multi-response optimization of oil extraction from pine nut (Pinus pinea L.) by response surface methodology: Extraction efficiency, physicochemical properties and antioxidant activity

Cited by 23 publications

References 23 publications

Preliminary Study: Comparison of Antioxidant Activity of Cannabidiol (CBD) and α-Tocopherol Added to Refined Olive and Sunflower Oils

Preliminary Study: Comparison of Antioxidant Activity of Cannabidiol (CBD) and α-Tocopherol Added to Refined Olive and Sunflower Oils

Antioxidant properties assessment of the cones of conifers through the combined evaluation of multiple antioxidant assays

Optimization and validation of a simplified methodology for simultaneous extraction of fatty acids and tocopherol homologues in peanuts

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