Multi-reassortant G3P[3] group A rotavirus in a horseshoe bat in Zambia

Sasaki, Michihito; Orba, Yasuko; Sasaki, S.; González, Gabriel; Ishii, Akihiro; Hang’ombe, Bernard M.; Mweene, Aaron S.; Ito, Kimihito; Sawa, Hirofumi

doi:10.1099/jgv.0.000591

Cited by 22 publications

(24 citation statements)

References 21 publications

(1 reference statement)

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“…However, the genetically close relationships of the VP7, VP4, VP6, NSP3 and NSP5 gene segments of the study strain to bat strain 3081/BRA and/or bat-like human strain Ecu534, suggest that RVA/Human-wt/SUR/2014735512/2013/G20P[28] is of bat origin. A few investigators have reported the detection of human RVAs genes in bat RVA strains (Esona et al, 2010; Sasaki et al, 2016; Waruhiu et al, 2017), but this report represents only the second time bat RVAs gene segments have been found in a human RVA strain (Solberg et al, 2009). …”

Section: Resultsmentioning

confidence: 93%

“…Several improvements in viral detection and characterization methodologies such as high-throughput next generation sequencing have led to a successful discovery and characterization of several novel viruses in bats, including enteric pathogens such as RVA (Luis et al, 2013; Waruhiu et al, 2017). However, since the first detection and characterization of a human-bat reassortant RVA from a Straw-colored Fruit bat in 2010 (Esona et al, 2010), a few of other bat RVA strains, detected and characterized from different bat species and different geographic locations have been reported (Asano et al, 2016; He et al, 2013; Sasaki et al, 2016; Waruhiu et al, 2017; Xia et al, 2013; Yinda et al, 2016). Among the reported bat RVA strains, only bat RVA strain 3081/BRA from Brazil was closely related in the VP7, NSP3 and NSP5 gene segments with the study strain from Suriname.…”

Section: Resultsmentioning

confidence: 99%

“…The nucleotide sequences of these bat RVAs are typically distant from those of human and other mammalian RVAs and have been suggested to be bat specific (Esona et al, 2010; He et al, 2013; Xia et al, 2014; Yinda et al, 2016). However, the VP4 and NSP4 gene segments of bat RVA strain RVA/Bat-wt/KEN/KE4852/2007/G25P[6] detected in Kenya (Esona et al, 2010), gene segments VP7, VP4, VP3, NSP2 and NSP3 of bat RVA strain RVA/Bat-wt/ZMB/LUS12–14/2012/G3P[3] reported in Zambia (Sasaki et al, 2016) and VP6 gene segment of bat RVA strains 2980/BatRVA and 322/BatRVA detected in Kenya (Waruhiu et al, 2017) are highly similar to cognate gene sequences of human RVAs circulating in the same community. This suggests that single or multiple gene reassortment events have occurred between human and bat RVAs.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of a human G20P[28] rotavirus a strain with multiple genes related to bat rotaviruses

Esona

Roy

Rungsrisuriyachai

et al. 2018

Infection, Genetics and Evolution

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Group A rotaviruses are the major cause of severe gastroenteritis in the young of mammals and birds. This report describes characterization of an unusual G20P[28] rotavirus strain detected in a 24 month old child from Suriname. Genomic sequence analyses revealed that the genotype constellation of the Suriname strain RVA/Human-wt/SUR/2014735512/2013/G20P[28] was G20-P[28]-I13-R13-C13-M12-A23-N13-T15-E20-H15. Genes VP1, VP2, VP3, NSP1, NSP2, NSP3, NSP4 and NSP5 were recently assigned novel genotypes by the Rotavirus Classification Working Group (RCWG). Three of the 11 gene segments (VP7, VP4, VP6) were similar to cognate gene sequences of bat-like human rotavirus strain Ecu534 from Ecuador and the VP7, NSP3 and NSP5 gene segments of strain RVA/Human-wt/SUR/2014735512/2013/G20P[28] were found to be closely related to gene sequences of bat rotavirus strain 3081/BRA detected in Brazil. Although distantly related, the VP1 gene of the study strain and bat strain BatLi09 detected in Cameroon in 2014 are monophyletic. The NSP1 gene was found to be most closely related to human strain QUI-35-F5 from Brazil. These findings suggest that strain RVA/Human-wt/SUR/2014735512/2013/G20P[28] represents a zoonotic infection from a bat host.

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular characterization of a human G20P[28] rotavirus a strain with multiple genes related to bat rotaviruses

Esona

Roy

Rungsrisuriyachai

et al. 2018

Infection, Genetics and Evolution

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“…During the subsequent decade, sporadic and scattered reports have been published about RVA strains in bats collected from serum, gut, and fecal samples in insectivorous and fruit bats. Several of these reports came from Chinese studies (24-27), but bat RVAs were also detected and (partially) characterized from France (28), Brazil (29), Zambia (30,31), Cameroon (32), Kenya (33), and Saudi Arabia (34). These studies investigated samples from a variety of bat families, such as Rhinolophidae (24, 30), Hipposideridae (25,26), Vespertilionidae (26,28), Molossidae, Phyllostomidae (29), Emballonuridae (26,33), Pteropodidae (23,(31)(32)(33)(34), and Rhinopomatidae (34).…”

mentioning

confidence: 99%

At Least Seven Distinct Rotavirus Genotype Constellations in Bats with Evidence of Reassortment and Zoonotic Transmissions

Simsek

Corman

Everling

et al. 2021

mBio

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Bats host many viruses pathogenic to humans, and increasing evidence suggests that rotavirus A (RVA) also belongs to this list. Rotaviruses cause diarrheal disease in many mammals and birds, and their segmented genomes allow them to reassort and increase their genetic diversity. Eighteen out of 2,142 bat fecal samples (0.8%) collected from Europe, Central America, and Africa were PCR-positive for RVA, and 11 of those were fully characterized using viral metagenomics. Upon contrasting their genomes with publicly available data, at least 7 distinct bat RVA genotype constellations (GCs) were identified, which included evidence of reassortments and 6 novel genotypes. Some of these constellations are spread across the world, whereas others appear to be geographically restricted. Our analyses also suggest that several unusual human and equine RVA strains might be of bat RVA origin, based on their phylogenetic clustering, despite various levels of nucleotide sequence identities between them. Although SA11 is one of the most widely used reference strains for RVA research and forms the backbone of a reverse genetics system, its origin remained enigmatic. Remarkably, the majority of the genotypes of SA11-like strains were shared with Gabonese bat RVAs, suggesting a potential common origin. Overall, our findings suggest an underexplored genetic diversity of RVAs in bats, which is likely only the tip of the iceberg. Increasing contact between humans and bat wildlife will further increase the zoonosis risk, which warrants closer attention to these viruses. IMPORTANCE The increased research on bat coronaviruses after severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV) allowed the very rapid identification of SARS-CoV-2. This is an excellent example of the importance of knowing viruses harbored by wildlife in general, and bats in particular, for global preparedness against emerging viral pathogens. The current effort to characterize bat rotavirus strains from 3 continents sheds light on the vast genetic diversity of rotaviruses and also hints at a bat origin for several atypical rotaviruses in humans and animals, implying that zoonoses of bat rotaviruses might occur more frequently than currently realized.

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“…During the subsequent decade, sporadic and scattered reports have been published about RVA strains in bats collected from serum, gut and faecal samples in insectivorous and fruit bats. Several reports of bat RVA strains came from Chinese studies (26)(27)(28)(29), but bat RVAs were also detected and (partially) characterized from France (30), Brazil (31), Zambia (32,33), Cameroon (34), Kenya (35) and Saudi-Arabia (36). These studies investigate (36) and Eidolon helvum (25,33,34,36).…”

Section: Introductionmentioning

confidence: 99%

At least seven distinct rotavirus genotype constellations in bats with evidence of reassortment and zoonotic transmissions

Simsek

Corman

Everling

et al. 2020

Preprint

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Bats host many viruses pathogenic to humans, and increasing evidence suggests that Rotavirus A (RVA) also belongs to this list. Rotaviruses cause diarrheal disease in many mammals and birds, and their segmented genomes allow them to reassort and increase their genetic diversity. Eighteen out of 2,142 bat faecal samples (0.8%) collected from Europe, South America and Africa were PCR-positive for RVA and 11 of those were fully characterized using viral metagenomics. Upon contrasting their genomes with publicly available data, at least 7 distinct bat RVA genotype constellations were identified, including evidence of reassortments among them. Some of these constellations are spread across the world, whereas others appear to be geographically restricted. Our analyses also provide evidence for multiple zoonotic transfer events involving bat RVAs. A Bulgarian bat RVAs possessed a genotype constellation previously identified in Chinese bats, and identical to an Argentinean horse RVA. A Costa Rican bat RVA possessed 3 previously undescribed gene segments and clustered closely with a human strain. Although SA11 is one of the most widely used reference strains for RVA research and forms the backbone of a reverse genetics system, its origin remained enigmatic. Remarkably, the majority of the gene segments of SA11 were closely related to Gabonese bat RVAs, suggesting a potential bat origin. Overall, our findings suggest an underexplored genetic diversity of RVAs in bats which is likely the tip of the iceberg. Increasing contact between humans and bat wildlife will further increase the zoonosis risk, which warrants closer attention to these viruses.

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Multi-reassortant G3P[3] group A rotavirus in a horseshoe bat in Zambia

Cited by 22 publications

References 21 publications

Molecular characterization of a human G20P[28] rotavirus a strain with multiple genes related to bat rotaviruses

Molecular characterization of a human G20P[28] rotavirus a strain with multiple genes related to bat rotaviruses

At Least Seven Distinct Rotavirus Genotype Constellations in Bats with Evidence of Reassortment and Zoonotic Transmissions

At least seven distinct rotavirus genotype constellations in bats with evidence of reassortment and zoonotic transmissions

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