Multi-Parameter Analysis of Spindle and Cell Cycle Dynamics in Asymmetric Cell Division

Plumb, K. W.; Pelletier, Vincent; Kaitna, Susi; Vogel, Jackie; Kilfoil, Maria

doi:10.1016/j.bpj.2008.12.605

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2015

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“…For cells treated with metabolic inhibitors, standard sodium azide and 2-deoxyglucose treatment was used. 21 All analysis was carried out using custom scripts in MATLAB (MathWorks). Images were acquired with a ImageEM EM-CCD camera (Hamamatsu) using a 63Â 1.4 NA objective.…”

Section: Methodsmentioning

confidence: 99%

Nonthermal fluctuations of the mitotic spindle

et al. 2015

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We present direct measurements of fluctuations in the nucleus of yeast cells. While prior work has shown these fluctuations to be active and non-thermal in character, their origin and time dependence are not understood. We show that the nuclear fluctuations we observe are quantitatively consistent with uncorrelated, active force fluctuations driving a nuclear medium that is dominated by an uncondensed DNA solution, for which we perform rheological measurements on an in vitro model system under similar conditions to what is expected in the nucleus.

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Section: Methodsmentioning

confidence: 99%