Multi-lineage differentiation of hMSCs encapsulated in thermo-reversible hydrogel using a co-culture system with differentiated cells

Park, Ji Sun; Yang, Han Na; Woo, Dae Gyun; Kim, Hye Min; Na, Kun; Park, Keun-Hong

doi:10.1016/j.biomaterials.2010.06.006

Cited by 18 publications

(4 citation statements)

References 39 publications

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“…CMA self-assembly and cold-denaturing temperatures match those of other previously established thermoreversible biomaterials, as it loses bulk mechanical properties at temperatures of less than 10 °C and forms a soft hydrogel (∼250 Pa) at physiological temperature. Current thermoreversible biomaterials are primarily synthetic hydrogels and have been utilized for tissue engineering applications as scaffolds for corneal wound repair, constructs for cell encapsulation, and drug delivery. − To our knowledge, CMA is the first thermoreversible, collagen-based hydrogel that has the ability to reversibly self-assemble into a fibrillar network at physiological temperature (above 20 °C) and is biodegradable through natural enzymes, biofunctional through cross-linking methods, and cytocompatible. , Future studies will aim to utilize these unique properties of CMA for soft tissue engineering applications.…”

Section: Discussionmentioning

confidence: 99%

Methacrylation Induces Rapid, Temperature-Dependent, Reversible Self-Assembly of Type-I Collagen

et al. 2014

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Type-I collagen self-assembles into a fibrillar gel at physiological temperature and pH to provide a cell-adhesive, supportive, structural network. As such, it is an attractive, popular scaffold for in vitro evaluations of cellular behavior and for tissue engineering applications. In this study, type-I collagen is modified to introduce methacrylate groups on the free amines of the lysine residues to create collagen methacrylamide (CMA). CMA retains the properties of collagen such as self-assembly, biodegradability, and natural bioactivity but is also photoactive and can be rapidly cross-linked or functionalized with acrylated molecules when irradiated with ultraviolet light in the presence of a photoinitiator. CMA also demonstrates unique temperature-dependent behavior. For natural type-I collagen, the overall structure of the fiber network remains largely static over time scales of a few hours upon heating and cooling at temperatures below its denaturation point. CMA, however, is rapidly thermoreversible and will oscillate between a liquid macromer suspension and a semisolid fibrillar hydrogel when the temperature is modulated between 10 and 37 °C. Using a series of mechanical, scattering, and spectroscopic methods, we demonstrate that structural reversibility is manifest across multiple scales from the protein topology of the triple helix up through the rheological properties of the CMA hydrogel. Electron microscopy imaging of CMA after various stages of heating and cooling shows that the canonical collagen-like D-periodic banding ultrastructure of the fibers is preserved. A rapidly thermoreversible collagen-based hydrogel is expected to have wide utility in tissue engineering and drug delivery applications as a biofunctional, biocompatible material. Thermal reversibility also makes CMA a powerful model for studying the complex process of hierarchical collagen self-assembly.

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Section: Discussionmentioning

confidence: 99%

Methacrylation Induces Rapid, Temperature-Dependent, Reversible Self-Assembly of Type-I Collagen

et al. 2014

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“…160 These hydrogels are nondegradable and have been investigated in vitro for cartilage formation by chondrocytes and MSCs under several conditions, such as the presence of growth factors or in coculture systems. 160,161 PNIPAAm hydrogels have also been modified with chitosan and with gelatin, allowing control over cell-matrix interactions. 162,163 Composite hydrogels have also been prepared with entangled hydrogel networks of poly(2-acrylamido-2-methylpropane sulfonic acid) and poly(N,N¢-dimethyl acrylamide).…”

mentioning

confidence: 99%

Hydrogels for the Repair of Articular Cartilage Defects

Spiller

Maher

Lowman

2011

Tissue Engineering Part B: Reviews

400

312

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“…A particularly interesting aspect of cell–environment interaction that has not been investigated much is spatially confined cell–cell interaction, where cells nearby can act as a source for bioactive molecules (i.e., paracrine interactions). Recent research on stem cells have also shown that the surrounding cell types play an important role in their differentiation tendencies 23–25…”

Section: Resultsmentioning

confidence: 99%

Stereolithography‐Based Hydrogel Microenvironments to Examine Cellular Interactions

Zorlutuna

Jeong

Kong

et al. 2011

Adv Funct Materials

120

107

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A spatially organized three‐dimensional (3D) co‐culture of multiple cell types is required to recapitulate cellular interactions and microenvironments in complex tissues. Although there are limited reports for 3D patterning of cells and materials, approaches to examine functional interactions of 3D spatially patterned multiple cell types are lacking entirely. This is mostly due to difficulties in controlling the physical arrangement of cells in a 3D matrix and the physical properties of the cell‐encapsulating matrix, while keeping the cells alive and functional for extended periods of time. In this study, an automated maskless fabrication technique is combined with a tunable polymer blend to spatially organize primary hippocampus neurons (HNs) and skeletal muscle myoblast cells (MCs) in a 3D hydrogel matrix with tunable mechanical and degradation properties. The spatial organization of these multiple cell types revealed that the presence of MCs resulted in increased cholinergic functionality of the HNs, as quantified by their choline acetyltransferase activity. The presence of a factor alone is not sufficient, but its spatiotemporal control is necessary; a condition that is possibly true for many cellular interactions. Therefore, the system described here offers a different approach to examine such previously unknown interactions. The approach proposed in this study can be used to examine interactions between many different cell types and shift the 3D fabrication paradigm to a next level, which is to fabricate tissues that are not only viable but also functional.

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Multi-lineage differentiation of hMSCs encapsulated in thermo-reversible hydrogel using a co-culture system with differentiated cells

Cited by 18 publications

References 39 publications

Methacrylation Induces Rapid, Temperature-Dependent, Reversible Self-Assembly of Type-I Collagen

Methacrylation Induces Rapid, Temperature-Dependent, Reversible Self-Assembly of Type-I Collagen

Hydrogels for the Repair of Articular Cartilage Defects

Stereolithography‐Based Hydrogel Microenvironments to Examine Cellular Interactions

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