Multi-isotope labelling of organic matter by diffusion of &amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt;H/&amp;lt;sup&amp;gt;18&amp;lt;/sup&amp;gt;O-H&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;O vapour and &amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C-CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; into the leaves and its distribution within the plant

Studer, Mirjam S.; Siegwolf, Rolf; Leuenberger, Markus; Abiven, Samuel

doi:10.5194/bg-12-1865-2015

Cited by 13 publications

(6 citation statements)

References 45 publications

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“…The soil water is the source of the two elements and their isotopes, and its uptake by the roots does not cause fractionation of both isotope compositions (White et al, 1985;Ehleringer and Dawson 1992;Dawson and Ehleringer 1993;Lin and Sternberg 1993). Evapotranspiration in the leaves causes enrichment in similar extent of the two heavy isotopes (Flanagan et al, 1991;Yakir 1992;McCarroll and Loader 2004;Studer et al, 2015), thus we can conclude that this does not cause the difference of δ 2 H between the two species but leaving the δ 18 O in the two species unchanged (Figure 2). The difference will FIGURE 4 | Isotopes in cellulose of living trees of 21 different species collected at the Bern Botanical Garden.…”

Section: Discussionmentioning

confidence: 86%

Larch Cellulose Shows Significantly Depleted Hydrogen Isotope Values With Respect to Evergreen Conifers in Contrast to Oxygen and Carbon Isotopes

Arosio¹,

Ziehmer-Wenz²,

Nicolussi³

et al. 2020

Front. Earth Sci.

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The analysis of the stable isotope of the tree-ring cellulose is an important tool for paleo climatic investigations. Long tree-ring chronologies consist predominantly of oaks and conifers in Europe, including larch trees (Larix decidua) and cembran pines (Pinus cembra) that form very long tree ring chronologies in the Alps and grow at the treeline, where tree growth is mainly determined by temperature variations. We analyzed δ13C, δ18O and δ2H isotopes in the cellulose extracted from tree-rings of wood samples collected at high altitude in the Swiss and Tyrol Alps, covering the whole Holocene period. We found that larch cellulose was remarkably more depleted in deuterium than that of cembran pine, with mean δ2H values of −113.4 ± 9.7‰ for larch and of −65.4 ± 11.3‰ for cembran pine. To verify if these depleted values were specific to larch or a property of the deciduous conifers, we extended the analysis to samples from various living conifer species collected at the Bern Botanical Garden. The results showed that not only the larch, but also all the samples of the deciduous larch family had a cellulose composition that was highly depleted in δ2H with regard to the other evergreen conifers including cembran pine, a difference that we attribute to a faster metabolism of the deciduous conifers. The δ18O values were not statistically different among the species, in agreement with the hypothesis that they are primary signals of the source water. While the δ13C values were slightly more depleted for larch than for cembran pine, likely due to metabolic differences of the two species. We conclude that the deciduous larch conifers have specific metabolic hydrogen fractionations and that the larch unique signature of δ2H is useful to recognize it from other conifers in subfossil wood samples collected for paleoclimatic studies. For climate information the absolute δ2H values of larch should be considered carefully and separate from other species.

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Section: Discussionmentioning

confidence: 86%

Larch Cellulose Shows Significantly Depleted Hydrogen Isotope Values With Respect to Evergreen Conifers in Contrast to Oxygen and Carbon Isotopes

Arosio¹,

Ziehmer-Wenz²,

Nicolussi³

et al. 2020

Front. Earth Sci.

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“…But in most cases, the allelopathic substances secreted by indigenous plants are mainly absorbed and utilized by the roots of invasive weeds and participate in cellular metabolic processes to inhibit their growth and development (Cheng and Cheng 2015). The distribution of the atom percent excess (APE) in different parts of A. philoxeroides was different, leaf > root > stem, and plant roots and stems produced more lignin and cellulose, which reduced the synthesis and utilization of allelopathic substances; it also related to the transport of the phloem of the stem, and A. philoxeroides enhanced photosynthesis in order to resist the stress damage of allelopathic substances, which led to the accumulation of more 13 C photosynthetic products in the leaf part (Studer et al 2015). The distribution of 13 C in the different parts of A. philoxeroides showed that the distribution of allelopathic substances absorbed by the plant in roots, stems and leaves was linked to the allelopathic inhibition of morphological characteristics (stem length, root length, node number, leaf number and leaf area).…”

Section: Discussionmentioning

confidence: 99%

Effects of 13C isotope-labeled allelochemicals on the growth of the invasive plant Alternanthera philoxeroides

Hua

et al. 2023

Preprint

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The secondary metabolites of indigenous plants have significant allelopathic inhibitory effects on the growth and development of invasive alien plants. Methyl palmitate (MP) and methyl linolenate (ML) of Humulus scandens root extracts were used as exogenous allelopathic substances. The research investigated the differences of allelopathic effects of MP and ML on the growth of seedlings of Alternanthera philoxeroides, and calculated their morphological characteristics, biomass, physiological indicators and the response index (RI). The synthetical allelopathic index (SE) of 1mmol/L MP was the smallest (-0.26) and the allelopathic inhibition was the strongest; therefore, it was selected as a 13C-labeled allelochemical. The distribution of 1mmol/L MP in different parts of A. philoxeroides and the correlation between the biomass ratios of roots, stems and leaves and the 13C content were studied by 13C stable isotope tracing experiments. Atom percent excess (APE) between roots, stems and leaves of A. philoxeroides treated with 1mmol/L MP were significantly different in terms of magnitude, with leaves (0.17%) > roots (0.12%) > stems (0.07%). The root, stem and leaf biomass ratios of invasive weeds had great significant positive correlation with 13C content (p < 0.01, R2 between 0.96 and 0.99). This current research provides a new idea and method for the control of A. philoxeroides, but large-scale popularization remains to be studied.

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“…The δ 13 C of water-soluble organic matter (WSOM) in leaves instead of bulk leaves was determined to represent the carbon isotope signals of photosynthates. Leaf WSOM has a high turnover rate [36][37][38] and mainly includes soluble carbohydrates, the hydrolysates of starch, and amino acids [39,40]. The extraction of WSOM referred to the protocols of [41].…”

Section: Determination Of Carbon Isotope Compositionmentioning

confidence: 99%

Species-Specific and Altitude-Induced Variation in Karst Plants’ Use of Soil Dissolved Inorganic Carbon

Rao

2022

Agronomy

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Root-derived carbon sources supporting photosynthesis have been demonstrated to contribute to plant carbon gain in many laboratory experiments. However, it remains largely unknown whether and to what extent soil dissolved inorganic carbon (DIC) influences leaf photosynthesis in karst habitats characterized by alkaline soils with low water content. We explored this relationship by measuring the concentrations and carbon isotope signals (δ13C) of soil DIC, as well as the δ13C of water-soluble organic matter (δWSOM) in leaves of nine woody species across an altitudinal gradient in karst habitats. The δWSOM varied among species by 7.23‰ and deviated from the δ13C of photosynthates solely assimilated from atmospheric CO2 (δA) by 0.44–5.26‰, with a mean value of 2.20‰. This systematical discrepancy (δA − δWSOM) could only be explained by the contribution of soil DIC to leaf total photosynthesis (fDIC_soil). The average values of fDIC_soil considerably varied among the nine species, ranging from 2.48% to 9.99%, and were comparable with or slightly lower than those of previous laboratory experiments. Furthermore, the fDIC_soil of two species significantly increased with altitude, whereas another species exhibited an opposite pattern, suggesting a highly spatial heterogeneity of DIC utilization. The present study improved our understanding of how plants adapt to the alkaline–drought soil conditions of karst habitats and thus acquire additional carbon for growth.

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Multi-isotope labelling of organic matter by diffusion of <sup>2</sup>H/<sup>18</sup>O-H<sub>2</sub>O vapour and <sup>13</sup>C-CO<sub>2</sub> into the leaves and its distribution within the plant

Cited by 13 publications

References 45 publications

Larch Cellulose Shows Significantly Depleted Hydrogen Isotope Values With Respect to Evergreen Conifers in Contrast to Oxygen and Carbon Isotopes

Larch Cellulose Shows Significantly Depleted Hydrogen Isotope Values With Respect to Evergreen Conifers in Contrast to Oxygen and Carbon Isotopes

Effects of 13C isotope-labeled allelochemicals on the growth of the invasive plant Alternanthera philoxeroides

Species-Specific and Altitude-Induced Variation in Karst Plants’ Use of Soil Dissolved Inorganic Carbon

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