Multi-Fractional Analysis of Molecular Diffusion in Polymer Multilayers by FRAP: A New Simulation-Based Approach

Sustr, David; Hlaváček, Antonín; Duschl, Claus; Volodkin, Dmitry

doi:10.1021/acs.jpcb.7b11051

Cited by 16 publications

(18 citation statements)

References 50 publications

(63 reference statements)

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“…In regards to this, the LbL assembly will provide a strong tool for introduction of various components into the assembled structures in order to tune a function of the finally-assembled structure. Novel approaches to probe diffusion into the formed multilayers are essential to understand and control a structure of the assemblies and release characteristics of BACs from the assembled structures [ 51 , 52 ].…”

Section: Resultsmentioning

confidence: 99%

Self-Assembled Mucin-Containing Microcarriers via Hard Templating on CaCO3 Crystals

et al. 2018

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Porous vaterite crystals of CaCO3 are extensively used for the fabrication of self-assembled polymer-based microparticles (capsules, beads, etc.) utilized for drug delivery and controlled release. The nature of the polymer used plays a crucial role and discovery of new perspective biopolymers is essential to assemble microparticles with desired characteristics, such as biocompatibility, drug loading efficiency/capacity, release rate, and stability. Glycoprotein mucin is tested here as a good candidate to assemble the microparticles because of high charge due to sialic acids, mucoadhesive properties, and a tendency to self-assemble, forming gels. Mucin loading into the crystals via co-synthesis is twice as effective as via adsorption into preformed crystals. Desialylated mucin has weaker binding to the crystals most probably due to electrostatic interactions between sialic acids and calcium ions on the crystal surface. Improved loading of low-molecular-weight inhibitor aprotinin into the mucin-containing crystals is demonstrated. Multilayer capsules (mucin/protamine)3 have been made by the layer-by-layer self-assembly. Interestingly, the deposition of single mucin layers (mucin/water)3 has also been proven, however, the capsules were unstable, most probably due to additional (to hydrogen bonding) electrostatic interactions in the case of the two polymers used. Finally, approaches to load biologically-active compounds (BACs) into the mucin-containing microparticles are discussed.

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Section: Resultsmentioning

confidence: 99%

Self-Assembled Mucin-Containing Microcarriers via Hard Templating on CaCO3 Crystals

et al. 2018

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“…In further studies, this approach was mainly applied for studying the diffusion of proteins in HA/PLL PEMs [ 108 , 109 , 110 ]. It was found that, similarly to the polymers that comprise PEMs, proteins loaded into PEMs also represent several fractions that have different degree of the binding to polymer network and therefore have different diffusivity ( Figure 4 B) [ 109 ].…”

Section: Analysis Of Polymer Dynamics Inside Pems: Methodsmentioning

confidence: 99%

“…Figure taken from reference [ 108 ], Copyright © 2020 Elsevier Ltd. ( B ) Distribution of diffusion coefficients of CytC loaded into (HA/PLL) 24 multilayers, where 24 is the number of polymer bilayers. Figure taken from reference [ 109 ], Copyright © 2020 American Chemical Society ( ).…”

Section: Figurementioning

confidence: 99%

Layer-By-Layer Assemblies of Biopolymers: Build-Up, Mechanical Stability and Molecular Dynamics

Campbell

Vikulina

2020

Polymers

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Rapid development of versatile layer-by-layer technology has resulted in important breakthroughs in the understanding of the nature of molecular interactions in multilayer assemblies made of polyelectrolytes. Nowadays, polyelectrolyte multilayers (PEM) are considered to be non-equilibrium and highly dynamic structures. High interest in biomedical applications of PEMs has attracted attention to PEMs made of biopolymers. Recent studies suggest that biopolymer dynamics determines the fate and the properties of such PEMs; however, deciphering, predicting and controlling the dynamics of polymers remains a challenge. This review brings together the up-to-date knowledge of the role of molecular dynamics in multilayers assembled from biopolymers. We discuss how molecular dynamics determines the properties of these PEMs from the nano to the macro scale, focusing on its role in PEM formation and non-enzymatic degradation. We summarize the factors allowing the control of molecular dynamics within PEMs, and therefore to tailor polymer multilayers on demand.

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“…Research activities in the area of LbL include planar films (Von Klitzing, 2006; Selin et al, 2018) and capsules. In addition to nanometer-thin LbL films, so-called micrometer thick exponentially grown LbL films were developed (Lavalle et al, 2004), which can host an enormous amount of both small and high molecular weight substances due to a large thickness of multilayers, e.g., those made of biopolymers (Sustr et al, 2015, 2018; Velk et al, 2016; Vikulina et al, 2016; Prokopovic et al, 2017). The uptake of the molecules, with various natures, is driven by the molecule interaction with free (uncompensated) charges of the inter-polymer complexes in the multilayers.…”

Section: Organic Chemical Matricesmentioning

confidence: 99%

Hierarchy of Hybrid Materials—The Place of Inorganics-in-Organics in it, Their Composition and Applications

et al. 2019

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Hybrid materials, or hybrids incorporating both organic and inorganic constituents, are emerging as a very potent and promising class of materials due to the diverse, but complementary nature of the properties inherent of these different classes of materials. The complementarity leads to a perfect synergy of properties of desired material and eventually an end-product. The diversity of resultant properties and materials used in the construction of hybrids, leads to a very broad range of application areas generated by engaging very different research communities. We provide here a general classification of hybrid materials, wherein organics– in -inorganics (inorganic materials modified by organic moieties) are distinguished from inorganics– in –organics (organic materials or matrices modified by inorganic constituents). In the former area, the surface functionalization of colloids is distinguished as a stand-alone sub-area. The latter area—functionalization of organic materials by inorganic additives—is the focus of the current review. Inorganic constituents, often in the form of small particles or structures, are made of minerals, clays, semiconductors, metals, carbons, and ceramics. They are shown to be incorporated into organic matrices, which can be distinguished as two classes: chemical and biological. Chemical organic matrices include coatings, vehicles and capsules assembled into: hydrogels, layer-by-layer assembly, polymer brushes, block co-polymers and other assemblies. Biological organic matrices encompass bio-molecules (lipids, polysaccharides, proteins and enzymes, and nucleic acids) as well as higher level organisms: cells, bacteria, and microorganisms. In addition to providing details of the above classification and analysis of the composition of hybrids, we also highlight some antagonistic yin-&-yang properties of organic and inorganic materials, review applications and provide an outlook to emerging trends.

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Multi-Fractional Analysis of Molecular Diffusion in Polymer Multilayers by FRAP: A New Simulation-Based Approach

Cited by 16 publications

References 50 publications

Self-Assembled Mucin-Containing Microcarriers via Hard Templating on CaCO3 Crystals

Self-Assembled Mucin-Containing Microcarriers via Hard Templating on CaCO3 Crystals

Layer-By-Layer Assemblies of Biopolymers: Build-Up, Mechanical Stability and Molecular Dynamics

Hierarchy of Hybrid Materials—The Place of Inorganics-in-Organics in it, Their Composition and Applications

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