Multi-enzyme complex of white rot fungi in saccharification of lignocellulosic material

Abstract

The multi-enzyme complex (crude extract) of white rot fungi Pleurotus ostreatus, Pleurotus eryngii, Trametes versicolor, Pycnosporus sanguineus and Phanerochaete chrysosporium were characterized, evaluated in the hydrolysis of pretreated pulps of sorghum straw and compared efficiency with commercial enzyme. Most fungi complexes had better hydrolysis rates compared with purified commercial enzyme.

Complete genome sequencing and comparative genome characterization of the lignocellulosic biomass degrading bacterium Pseudomonas stutzeri MP4687 from cattle rumen

“…While, similar activities was reported into the various fungal strains isolated from agriculture waste or forest. The crude extract of white rot fungi Pleurotus ostreatus, Pleurotus eryngii, Trametes versicolor, Pycnosporus sanguineus and Phanerochaete chrysosporium were showed enzyme activites in similar range or less on pretreated pulps of sorghum straw [ 27 ]. The rumen is a natural fermenter having a role in plant polysaccharide processing.…”

Edible Mushroom: A Potent Producer of Industrial Enzymes

“…Similar trend was observed in case of hemicellulolytic enzyme genes, lignin degrading auxiliary enzyme genes and lignin oxidase genes. Saccharification of the pulp from straw forage sorghum pre-treated by acid and delignification by edible mushroom's multienzyme complex was higher in Trametes versicolor TRAM01 and Pleurotus ostreatus PLO06 compare to the commercial enzyme (Table III) (Cardosos & de Queiroz, 2018).…”

Cultivation of Mushrooms and Their Lignocellulolytic Enzyme Production Through the Utilization of Agro-Industrial Waste

“…Many agricultural or natural lignocellulosic solid waste, especially different kinds of straw (wheat, sorghum, rice) and sawdust (oak and pine), were used as a substrate or source for mushroom growth and cellulases production [150][151][152][153]. Furthermore, other forms of lignocellulosic waste, such as peanut hulls, mandarin peels, cotton waste, corn stovers and tree leaves (Fagus sylvatica), have also been used as substrates to determine cellulase activity [150,154,155]. The high-value potential of these forms of waste is encouraging as they can be sources that support the growth and cellulases production of different mushroom species, namely Ganoderma, Grifola, Lentinula, Lentinus, Pleurotus, Piptoporus and Trametes by SSF [152,[156][157][158][159].…”

“…Lignocellulolytic enzymes have received attention because of their potential applications in various agro-industrial bioprocesses, such as the conversion of hemicellulosic biomass to fuels and chemical production, the clarification of juices, the green processing of certain foods and beverages, the enhancement of animal digestibility in feedstock, the delignification of paper and pulp, the improvement of fabric properties in the textile industry and waste utilization [277][278][279]. Cellulase is widely used in the textile and laundry detergent industries as it can play a part in the hydrolysis of cellulose and improve fabric properties for the textile industry and for cleaning textiles in the laundry detergent industry [154,280]. The food and beverage processing industries have used cellulase for the hydrolysis of cellulose during the drying of coffee beans and for the extraction of fruits and vegetables in juice production [281,282].…”