Multi-color polymer pen lithography for oligonucleotide arrays

Kumar, Ravi; Weigel, Simone; Meyer, Rebecca; Niemeyer, Christof M.; Fuchs, Harald; Hirtz, Michael

doi:10.1039/c6cc07087f

Cited by 27 publications

(34 citation statements)

References 31 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, traditional PPL limits the creation of complex patterns. Therefore, consecutive PPL processes with intermediate inking steps can extend the number of immobilized features deposited over the same area, promising a good alternative to create multiplexed PA …”

Section: Introductionmentioning

confidence: 99%

Highly Anisotropic Suspended Planar‐Array Chips with Multidimensional Sub‐Micrometric Biomolecular Patterns

Agusil

Torras

Duch

et al. 2017

Adv Funct Materials

View full text Add to dashboard Cite

Suspended planar-array (SPA) chips embody millions of individual miniaturized arrays to work in extremely small volumes. Here, the basis of a robust methodology for the fabrication of SPA silicon chips with on-demand physical and chemical anisotropies is demonstrated. Specifically, physical traits are defined during the fabrication process with special focus on the aspect ratio, branching, faceting, and size gradient of the final chips. Additionally, the chemical attributes augment the functionality of the chips with the inclusion of complete coverage or patterns of selected biomolecules on the surface of the chips with contact printing techniques, offering an extremely high versatility, not only with the choice of the pattern shape and distribution but also in the choice of biomolecular inks to pattern. This approach increases the miniaturization of printed arrays in 3D structures by two orders of magnitude compared to those previously demonstrated. Finally, functional micrometric and sub-micrometric patterned features are demonstrated with an antibody binding assay with the recognition of the printed spots with labeled antibodies from solution. The selective addition of physical and chemical attributes on the suspended chips represents the basis for future biomedical assays performed within extremely small volumes.

show abstract

Section: Introductionmentioning

confidence: 99%

Highly Anisotropic Suspended Planar‐Array Chips with Multidimensional Sub‐Micrometric Biomolecular Patterns

Agusil

Torras

Duch

et al. 2017

Adv Funct Materials

View full text Add to dashboard Cite

show abstract

“…This approach could prove highly useful in potentially multiplexing different colors or chemical functionalities rapidly over large areas (>mm 2 ) with nanoscale feature sizes (sub-100 nm), a characteristic that is highly desirable for a broad range of applications ranging from medical diagnostic systems to optoelectronic devices. [33][34][35]…”

Section: Resultsmentioning

confidence: 99%

Super-resolution interference lithography enabled by non-equilibrium kinetics of photochromic monolayers

et al. 2019

View full text Add to dashboard Cite

show abstract

“…After this, the BSA‐coated substrates were ready to use for the microarray printing. The plasma‐cleaned PDMS stamp of area 10 × 10 mm 2 was levelled and contacted once to the substrate for printing patterns having 50 µm distance between ink dots. Then the stamp was moved by 10 mm and contacted again to obtain a large area biotin dots pattern next to the already printed area.…”

Section: Methodsmentioning

confidence: 99%

“…The microarray within the microfluidic channel was printed via polymer pen lithography (PPL) . This technique combines aspects of microcontact printing and dip‐pen nanolithography (DPN) in a hybrid way and allows large area (several cm²) patterning, especially for sensitive, bioactive molecules in gradients and in a multiplexed fashion . This allows also easy integration into microfluidic systems as we demonstrated for the above‐mentioned application of CTC capture and mast cell screening, recently.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Microfluidic Ceiling Designs for the Capture of Circulating Tumor Cells on a Microarray Platform

et al. 2019

View full text Add to dashboard Cite

The capture of circulating tumor cells (CTCs) is still a challenging application for microfluidic chips, as these cells are rare and hidden in a huge background of blood cells. Here, different microfluidic ceiling designs in regard to their capture efficiency for CTCs in model experiments and more realistic conditions of blood samples spiked with a clinically relevant amount of tumor cells are evaluated. An optimized design for the capture platform that allows highly efficient recovery of CTCs from size‐based pre‐enriched samples under realistic conditions is obtained. Furthermore, the viability of captured tumor cells as well as single cell recovery for downstream genomic analysis is demonstrated. Additionally, the authors' findings underline the importance of evaluating rational design rules for microfluidic devices based on theoretical models by application‐specific experiments.

show abstract

Multi-color polymer pen lithography for oligonucleotide arrays

Cited by 27 publications

References 31 publications

Highly Anisotropic Suspended Planar‐Array Chips with Multidimensional Sub‐Micrometric Biomolecular Patterns

Highly Anisotropic Suspended Planar‐Array Chips with Multidimensional Sub‐Micrometric Biomolecular Patterns

Super-resolution interference lithography enabled by non-equilibrium kinetics of photochromic monolayers

Evaluation of Microfluidic Ceiling Designs for the Capture of Circulating Tumor Cells on a Microarray Platform

Contact Info

Product

Resources

About