Mulching practices manipulate the microbial community diversity and network of root‑associated compartments in the Loess Plateau

Tian, Lixin; Yu, Shen; Zhang, Lei; Dong, Kongjun; Feng, Baili

doi:10.1016/j.still.2022.105476

Cited by 22 publications

(22 citation statements)

References 64 publications

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“…Extracellular enzymes have an essential role in energy flow and material transformation in agro‐ecosystems, which are closely associated with soil conditions and are widely adopted to assess soil biochemical activity and soil quality (Liu et al, 2017). As demonstrated in our previous work (Tian, Yu, et al, 2022), RF and PFM treatments considerably improved the soil temperature and moisture (Table 1). C‐acquiring enzymes (i.e., BG and CBH) are highly sensitive to variations in the soil hydrothermal conditions (Ren et al, 2017).…”

Section: Discussionsupporting

confidence: 78%

“…The trial was set up in 2020 with three repetitions per regime (28 m 2 per regime) with a randomised block design and included three agricultural technologies: (i) traditional flat planting with non‐mulch (TP); (ii) ridge‐furrow plastic film mulching (RF); and (iii) plastic film mulching (PFM), our previous study reported the specific mulching methods (Figure S1, Tian, Yu, et al, 2022). Broomcorn millet variety “Yumi No.…”

Section: Methodsmentioning

confidence: 99%

“…Rhizosphere soil was collected using a sterilised shovel from approximately 10 plants in each plot. Plant roots were shaken to discard bulk soil, and rhizosphere soil attached to the roots about 2 mm were obtained and merged into one sample (Tian, Yu, et al, 2022). Rhizosphere soil was divided into two parts: one part was placed at −20°C for analysis of soil properties and enzymatic activities, and the other part was placed at −80°C for DNA extraction.…”

Section: Methodsmentioning

confidence: 99%

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Use of metagenomics and metabolomics to quantify changes in rhizosphere soil microbial function in response to mulching regimes

Tian

Zhang

et al. 2023

Land Degrad Dev

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The effects of distinct mulching regimes on the microbial functional properties and metabolic profiles of rhizosphere soil remain largely unknown. Here, we conducted metagenomic and metabolomic analyses to explore the microbial functional specificity and metabolic pathways under three mulching regimes, namely, traditional flat planting with non‐mulch (TP), ridge‐furrow plastic film mulching (RF), and plastic film mulching (PFM). Compared to TP treatment, RF and PFM treatments significantly improved the soil temperature and moisture content, stimulated the increase of five extracellular enzyme activities, thereby reducing soil nutrient content (soil pH, total N, soil organic carbon, NH4+‐N, and NO3−‐N) to meet crop demand for nutrients. In addition, RF treatment significantly decreased the relative abundance of nxrA, while increasing the relative abundance of nasA, thereby further decreasing NO3−‐N content. However, RF and PFM treatments had no significant effect on the relative abundance of nirB, nirD, nrfA, and nrfH. Meanwhile, PFM and RF treatment stimulated the increase of extracellular enzyme activities accelerated microbial metabolism, thereby promoting the synthesis of metabolites involved in carbohydrate metabolism, lipid metabolism, nucleotide metabolism, and amino acid metabolism. Metabolomics and network analyses revealed that RF and PFM treatments not only enriched organoheterocyclic compounds, organic acids and derivatives, lipids and lipid‐like molecules, and organic oxygen compounds but also increased their relative abundance in the major modules. Overall, this study provides deep insights into how mulching regimes alter soil microbial functional potential and metabolic profiles, which may have predictable impacts on the rhizosphere soil nutrient cycling of agroecosystems under mulching systems in semiarid regions.

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Section: Discussionsupporting

confidence: 78%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Use of metagenomics and metabolomics to quantify changes in rhizosphere soil microbial function in response to mulching regimes

Tian

Zhang

et al. 2023

Land Degrad Dev

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“…The trial was set up in 2020 with three repetitions per regime (28 m 2 per regime) with a randomized block design, and included three agricultural technologies:(i) no mulching (NM); (ii) ridge‐furrow mulching system (RF); (iii) plastic FM, our previous study reported the specific mulching methods (Figure S1, Tian et al, 2022). Proso millet variety “Yumi No.…”

Section: Methodsmentioning

confidence: 99%

Bacterial communities in proso millet root‐associated compartments are regulated by growth period and mulching regime

et al. 2022

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Mulching regimes have a promoted impact on the soil hydrothermal environments and crop productions. However, the time‐dependent variations in root‐associated microbial communities under distinct mulching regimes in the Loess Plateau have not been thoroughly explored. Here, we investigated the regulatory impacts of plastic film mulching (FM) and ridge‐furrow mulching system (RF) on the microbial community compositions, diversities, and ecological networks in the distinct compartments (e.g., root, rhizosphere, and bulk soil) at jointing and flowering periods of proso millet, and we compared these two regimes to no mulching (NM). Mulching regimes improved the bulk and rhizosphere soil quality at jointing period, whereas reduced them at flowering period. Bacterial structure and composition of the three compartments were significantly influenced by growth periods and mulching regimes. Furthermore, mulching regimes effectively increased the alpha diversities of rhizosphere and root communities at flowering period. Soil temperature and total N were the most essential variables affecting the bulk soil and rhizosphere community structure, respectively. With the growth and development of proso millet, the network complexity and stability increased, and the abundance of different modules in the three compartments were regulated by mulching regimes. Proteobacteria and Acidobacteriota were the keystone species at jointing period, whereas, at flowering period, only Proteobacteria were the keystone species. In addition, FM regime significantly increased the species interaction and abundance of keystone taxa than those of non‐mulching at both periods. These results highlighted that mulching regimes were observed to be a recommended selection for improving soil quality, regulating bacterial diversity and structure, and stabilizing the species interaction, thereby providing guidance for choosing the ideal cultivation techniques from the perspective of microbial ecology in the Loess Plateau.

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“…At each site, three 20‐m 2 plots were selected, and the rhizosphere soil was obtained with a spade from randomly selected 10–15 healthy plant roots at a depth of 0–20 cm from each plot. The plant roots were carefully shaken to discard large soil particles, and the rhizosphere soil attached to the plant roots approximately 0.2 cm was carefully collected and merged into one rhizosphere sample, and placed in a sterile ziplock bag (Tian et al, 2022). All soil samples were sieved through <2‐mm sieve, transported to the laboratory on ice, and stored at −80°C before physiochemical analysis and DNA extraction.…”

Section: Methodsmentioning

confidence: 99%

Rhizosphere bacterial communities of three minor grain crops exhibit distinct environmental adaptations and assembly processes

Tian¹,

Zhang²,

Chen³

et al. 2022

European J Soil Science

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Deciphering responses of bacterial communities to environmental change, and understanding how communities assemble in response to environmental change, are important subjects. The assembly processes governing the rhizosphere bacterial communities of minor grain crops are rarely explored based on regional scales, especially in terms of the environmental adaptation. Here, we investigated the environmental thresholds and phylogenetic signals for ecological preferences of rhizosphere bacterial communities of three minor grain crop taxa across complex environmental gradients to reflect their environmental adaptation. Additionally, we reported environmental factors affecting their community assembly processes based on a large‐scale soil survey in agricultural fields across northern China using high‐throughput sequencing. The results demonstrated a narrower range of environmental thresholds and weaker phylogenetic signals for the ecological traits of rhizosphere bacteria in proso millet than in foxtail millet and sorghum fields. The proso millet rhizosphere community was the most phylogenetically clustered. Null model analysis indicated that homogeneous selection belonging to deterministic processes governed the sorghum rhizosphere community, whereas dispersal limitation belonging to stochastic processes was the critical assembly process in the foxtail millet and proso millet. Mean annual temperature was the decisive factor for adjusting the balance between stochasticity and determinism of the foxtail millet, proso millet and sorghum rhizosphere communities. A higher temperature resulted in stochasticity in the proso millet and sorghum communities. For the foxtail millet community, the deterministic assembly increased with an increase in temperature. These results contribute to the understanding of rhizosphere‐associated bacterial community assembly processes in agro‐ecosystems on a large scale. Highlights Proso millet rhizosphere bacterial taxa exhibited weaker environmental adaptation than foxtail millet and sorghum taxa. Determinism governed the sorghum rhizosphere community, whereas stochasticity was the critical assembly process in the foxtail and proso millet. Mean annual temperature mediated balance between stochastic and deterministic processes.

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Mulching practices manipulate the microbial community diversity and network of root‑associated compartments in the Loess Plateau

Cited by 22 publications

References 64 publications

Use of metagenomics and metabolomics to quantify changes in rhizosphere soil microbial function in response to mulching regimes

Use of metagenomics and metabolomics to quantify changes in rhizosphere soil microbial function in response to mulching regimes

Bacterial communities in proso millet root‐associated compartments are regulated by growth period and mulching regime

Rhizosphere bacterial communities of three minor grain crops exhibit distinct environmental adaptations and assembly processes

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