Mueller Matrix Analysis of Collagen and Gelatin Containing Samples Towards More Objective Skin Tissue Diagnostics

Fricke, Dierk; Becker, Alexander; Heratizadeh, Annice; Knigge, Sara; Jütte, Lennart; Meinhardt‐Wollweber, Merve; Werfel, Thomas; Roth, Bernhard; Glasmacher, Birgit

doi:10.3390/polym12061400

Cited by 6 publications

(15 citation statements)

References 26 publications

(52 reference statements)

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“…The relative velocity, at the surface of the collector, is of special interest for this study. As shown by Fricke et al (2019), an increase in relative collector velocity leads to an increased degree of fiber orientation [ 9 , 14 ].…”

Section: Methodsmentioning

confidence: 99%

“…The used electrospinning device (see Figure 1 ) was assembled according to Fricke et al (2019) and is composed of a syringe pump, a syringe, polyethylene tubing, a blunt cannula, and an electric motor driving the rotating drum collector [ 6 , 9 , 14 ].…”

Section: Methodsmentioning

confidence: 99%

“…The samples were manufactured out of a solution of polycaprolactone (PCL, 80 kDa, Sigma-Aldrich Chemistry Corporate, St. Louis, MO, US) in 2,2,2-TFE (99.8%, abcr GmbH, Karlsruhe, Germany) with a concentration of 17% ( w / v ) (170 mg/mL). The solution was prepared according to Fricke et al (2019) [ 9 , 14 ]. The samples were fabricated with a 250 mm tip-to-collector distance, voltage of 20 kV, relative collector velocities of 2 m/s and 8 m/s, and a process duration of 120 min ( Figure 1 ).…”

Section: Methodsmentioning

confidence: 99%

“…All values located outside of the 1.5 × IQR are individually displayed as dots. These were defined as outliers, with regard to the IQR, but do not necessarily have to be considered as extreme values [ 9 ].…”

Section: Methodsmentioning

confidence: 99%

“…With this in mind, polymers are commonly used materials in TE [ 6 , 7 , 8 ]. In fact, the fabrication of fiber scaffolds by electrospinning (see Figure 1 ) has produced great results for several different combinations of polymers and solvents (e.g., polycaprolactone (PCL) in trifluoroethanol (TFE) or polyethylene oxide in water) [ 9 , 10 , 11 ]. The resulting fibers inherit diameters between a few hundred nanometers and a few microns [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

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In Situ Characterization of Polycaprolactone Fiber Response to Quasi-Static Tensile Loading in Scanning Electron Microscopy

et al. 2021

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Microstructural responses to the mechanical load of polymers used in tissue engineering is notably important for qualification at in vivo testing, although insufficiently studied, especially regarding promising polycaprolactone (PCL). For further investigations, electrospun PCL scaffolds with different degrees of fiber alignment were produced, using two discrete relative drum collector velocities. Development and preparation of an adjusted sample geometry enabled in situ tensile testing in scanning electron microscopy. By analyzing the microstructure and the use of selected tracking techniques, it was possible to visualize and quantify fiber/fiber area displacements as well as local fractures of single PCL fibers, considering quasi-static tensile load and fiber alignment. The possibility of displacement determination using in situ scanning electron microscopy techniques for testing fibrous PCL scaffolds was introduced and quantified.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In Situ Characterization of Polycaprolactone Fiber Response to Quasi-Static Tensile Loading in Scanning Electron Microscopy

et al. 2021

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Monitoring of multiple fabrication parameters of electrospun polymer fibers using mueller matrix analysis

Sharma,

Jütte,

Gopani

et al. 2024

J. Opt.

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Electrospun polymer fiber mats feature versatile applications in tissue engineering, drug delivery, water treatment and chemical processes. The orientation of fibers within these mats is a crucial factor that significantly influences their properties and performance. However, the analysis of fiber samples using scanning electron microscopy (SEM) has limitations such as time consumption, fixed assembly, and restricted field of vision. Therefore, a fast and reliable method for qualitative measurements of fiber orientation is required. Mueller matrix polarimetry, a well-established method for measuring orientation of chemical and biological species, was employed in this case.We investigated the effect of four important parameters of the electrospinning process, namely collector speed, applied voltage, needle-to-collector distance, and solution concentration, on fiber orientation using Mueller matrix polarimetry thus extending the range of parameters analysed.  Measurements were performed using two extreme values and a central optimized value for each fabrication parameter. Changes in matrix values were observed for each fabrication parameter, and their correlation with fiber orientation was analyzed based on the Lu-Chipman decomposition. The results were compared with SEM images, which served as the ground truth, and showed overall good agreement. In the future, the analysis of electrospun polymer fibers can be done by using Mueller matrix polarimetry as alternative to current technology and fabrication parameters, including solution concentration for the first time in this context and the production can quickly be adjusted based on the outcome of the measurements. 

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Improved polarimetric analysis of human skin through stitching: advantages, limitations, and applications in dermatology

Jütte,

Patel,

Roth

2023

Biomed. Phys. Eng. Express

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Polarimetry is a powerful tool for the analysis of the optical properties of materials and systems, such as human skin. However, in many polarimetric setups, the field of view is limited to a few square centimeters. In these cases, it is possible to resort to stitching techniques, which involve combining multiple Mueller matrix measurements obtained from different overlapping regions of the sample. In this paper, we propose a stitching technique for polarimetric data and discuss its advantages and limitations. We also describe the potential of image stitching for improving the accuracy and robustness of in vivo polarimetry in the presence of random patient movement. We conducted our research using a diverse set of samples which included porcine skin, human skin from arms and fingers, cold cuts of chicken and gelatine, alongside synthetically created sample data. Our results demonstrate the effectiveness of this technique for the application in dermatology. Each additional in vivo measurement enhances the field of view by approximately one third, thereby considerably augmenting the total observation area. We show that stitching enables for the polarimetric assessment of large skin patches which is useful for the diagnosis of inflammatory skin diseases.

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Mueller Matrix Analysis of Collagen and Gelatin Containing Samples Towards More Objective Skin Tissue Diagnostics

Cited by 6 publications

References 26 publications

In Situ Characterization of Polycaprolactone Fiber Response to Quasi-Static Tensile Loading in Scanning Electron Microscopy

In Situ Characterization of Polycaprolactone Fiber Response to Quasi-Static Tensile Loading in Scanning Electron Microscopy

Monitoring of multiple fabrication parameters of electrospun polymer fibers using mueller matrix analysis

Improved polarimetric analysis of human skin through stitching: advantages, limitations, and applications in dermatology

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