2017
DOI: 10.1093/mutage/gex003
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Abstract: Our previous results showed that in addition to the immediate interaction of ionising radiation with DNA (direct and indirect effect), low-dose and chronic low-dose rate of irradiation induce endogenous oxidative stress. During oxidative stress, free radicals react with DNA, nucleoside triphosphates (dNTPs), proteins and lipids, and modify their structures. The MYH and MTH1 genes play important roles in preventing mutations induced by 8-hydroxy-guanine, which is an oxidised product of guanine. In this study, w… Show more

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Cited by 12 publications
(12 citation statements)
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“…The results are in accordance with our previous report, where we demonstrated that exposure of cells to low dose rates of gamma radiation led to oxidative stress and increased production of 8-oxo-dGTP, which is released to the medium as 8-oxo-dG. In cells exposed to 0.5 and 1 Gy gamma rays (high dose rate), increased levels of 8-oxo-dG were not observed [69]. Since cellular responses to ionizing radiation depend on DNA damage processing and repair, these processes are likely to be involved in the differential cellular responses regarding radiation qualities.…”
Section: Discussionsupporting
confidence: 93%
See 1 more Smart Citation
“…The results are in accordance with our previous report, where we demonstrated that exposure of cells to low dose rates of gamma radiation led to oxidative stress and increased production of 8-oxo-dGTP, which is released to the medium as 8-oxo-dG. In cells exposed to 0.5 and 1 Gy gamma rays (high dose rate), increased levels of 8-oxo-dG were not observed [69]. Since cellular responses to ionizing radiation depend on DNA damage processing and repair, these processes are likely to be involved in the differential cellular responses regarding radiation qualities.…”
Section: Discussionsupporting
confidence: 93%
“…NRF2 regulates basal and inducible expression of enzymes, controlling key components of endogenous antioxidant systems [34]. Considering that low dose rate irradiation induces oxidative damage [69], we knocked down NRF2 under the hypothesis that this might increase the sensitivity of neurospheres to oxidative stress at low dose rate radiation. After knockdown, NRF2 expression remained stable with an average of 79% lower expression than the corresponding NRF2 wild-type cells.…”
Section: Discussionmentioning
confidence: 99%
“…8-Oxo-dG is produced when ROS, for example, hydroxyl radicals, react with guanine in DNA or deoxyguanosine triphosphate (dGTP) in the cytoplasm. We have previously shown that the origin of extracellular 8-oxo-dG is the cytoplasmic content of dGTP which can be converted into 8-oxo-dGTP by free radical attack [ 3 5 ]. 8-Oxo-dG is excreted into extracellular environments such as urine, blood, and saliva.…”
Section: Introductionmentioning
confidence: 99%
“…8-oxo-dG was analysed using a 8-oxo-dG enzyme-linked immunosorbent assay (ELISA). Prior to this assay, 500 µl of saliva was purified twice on a C18 solid phase extraction column (Varian, Lake Forest, CA, USA) as described by Shakeri Manesh et al 99 . The 8-oxo-dG ELISA (Health Biomarkers Sweden AB, Stockholm, Sweden) was performed as described by Haghdoost et al 78 .…”
Section: Buccal Mucosal Cell Collection and Immunocytological Staininmentioning
confidence: 99%