Msx1 is required for the induction ofPatched bySonic hedgehog in the mammalian tooth germ

Zhang, Yanding; Zhao, Xiang; Hu, Yueping; Amand, Tara St.; Zhang, Meifeng; Ramamurthy, Rajee; Qiu, Mengsheng; Chen, Yiping

doi:10.1002/(sici)1097-0177(199905)215:1<45::aid-dvdy5>3.0.co;2-5

Cited by 76 publications

(9 citation statements)

References 46 publications

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“…Skeletal preparations were performed as previously described (Martin et al, 1995). Whole mount and section in situ hybridization was performed as previously described (Zhang et al, 1999). At least three embryos of each genotype were hybridized to each probe and only probes that detected consistent patterns of expression in all samples were considered as valid results.…”

Section: Methodsmentioning

confidence: 99%

Hedgehog signaling patterns the oral-aboral axis of the mandibular arch

Liu

Lan

et al. 2019

eLife

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Development of vertebrate jaws involves patterning neural crest-derived mesenchyme cells into distinct subpopulations along the proximal-distal and oral-aboral axes. Although the molecular mechanisms patterning the proximal-distal axis have been well studied, little is known regarding the mechanisms patterning the oral-aboral axis. Using unbiased single-cell RNA-seq analysis followed by in situ analysis of gene expression profiles, we show that Shh and Bmp4 signaling pathways are activated in a complementary pattern along the oral-aboral axis in mouse embryonic mandibular arch. Tissue-specific inactivation of hedgehog signaling in neural crest-derived mandibular mesenchyme led to expansion of BMP signaling activity to throughout the oral-aboral axis of the distal mandibular arch and subsequently duplication of dentary bone in the oral side of the mandible at the expense of tongue formation. Further studies indicate that hedgehog signaling acts through the Foxf1/2 transcription factors to specify the oral fate and pattern the oral-aboral axis of the mandibular mesenchyme.

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Section: Methodsmentioning

confidence: 99%

Hedgehog signaling patterns the oral-aboral axis of the mandibular arch

Liu

Lan

et al. 2019

eLife

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“…cDMA fragments were subcloned into pBluesscript for riboprobe transcription. Mouse Shh , Ptch1 , Patch2 , Smo , Gli1 , Gli2, and Gli3 probes were used to detect gene expression in the mouse tooth germ [12,28]. Section in situ hybridization with DIG-labeled riboprobe was carried out as described previously [3,28].…”

Section: Methodsmentioning

confidence: 99%

Expression of SHH signaling molecules in the developing human primary dentition

Zhang

Chen

et al. 2013

BMC Dev Biol

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BackgroundOur current knowledge on tooth development derives primarily from studies in mice. Very little is known about gene expression and function during human odontogenesis. Sonic Hedgehog (SHH) signaling has been demonstrated to play crucial roles in the development of multiple organs in mice, including the tooth. However, if SHH signaling molecules are expressed and function in the developing human embryonic tooth remain unknown.ResultsWe conducted microarray assay to reveal the expression profile of SHH signaling pathway molecules. We then used in situ hybridization to validate and reveal spatial and temporal expression patterns of a number of selected molecules, including SHH, PTC1, SMO, GLI1, GLI2, and GLI3, in the developing human embryonic tooth germs, and compared them with that in mice. We found that all these genes exhibit similar but slightly distinct expression patterns in the human and mouse tooth germ at the cap and bell stages.ConclusionsOur results demonstrate the operation of active SHH signaling in the developing human tooth and suggest a conserved function of SHH signaling pathway during human odontogenesis.

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“…In situ hybridization was performed as previously described [41]. The human cDNA vectors for WNT2 WNT7B FZD7 LRP6 DVL2 DLV3 GSK-3β APC AXIN2 and TCF4 digoxygenin-labelled probes were amplified from cDNA templates, which were synthesized from total RNA derived from human lung at 17 W. Sequences of the PCR primers and the enzyme sites used for insertion into the pBluescriptII KS plasmid are shown in Table 2.…”

Section: Methodsmentioning

confidence: 99%

Expression of canonical WNT/β-CATENIN signaling components in the developing human lung

et al. 2012

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BackgroundThe WNT/β-CATENIN signaling cascade is crucial for the patterning of the early lung morphogenesis in mice, but its role in the developing human lung remains to be determined. In this study, expression patterns of canonical WNT/β-CATENIN signaling components, including WNT ligands (WNT2, WNT7B), receptors ( FZD4, FZD7, LRP5, LRP6), transducers ( DVL2, DVL3, GSK-3β, β-CATENIN, APC, AXIN2), transcription factors ( TCF4, LEF1) and antagonists ( SOSTDC1) were examined in human embryonic lung at 7, 12, 17 and 21 weeks of gestation (W) by real-time qRT-PCR and in situ hybridization.ResultsqRT-PCR analysis showed that some of these components were gradually upregulated, while some were significantly downregulated from the 7 W to the 12 W. However, most components reached a high level at 17 W, with a subsequent decrease at 21 W. In situ hybridization showed that the canonical WNT ligands and receptors were predominantly located in the peripheral epithelium, whereas the canonical WNT signal transducers and transcription factors were not only detected in the respiratory epithelium, but some were also scattered at low levels in the surrounding mesenchyme in the developing human lung. Furthermore, Western blot, qRT-PCR and histological analysis demonstrated that the β-CATENIN-dependent WNT signaling in embryonic human lung was activated in vitro by CHIR 99021 stimulation.ConclusionsThis study of the expression patterns and in vitro activity of the canonical WNT/β-CATENIN pathways suggests that these components play an essential role in regulation of human lung development.

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Msx1 is required for the induction ofPatched bySonic hedgehog in the mammalian tooth germ

Cited by 76 publications

References 46 publications

Hedgehog signaling patterns the oral-aboral axis of the mandibular arch

Hedgehog signaling patterns the oral-aboral axis of the mandibular arch

Expression of SHH signaling molecules in the developing human primary dentition

Expression of canonical WNT/β-CATENIN signaling components in the developing human lung

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