MSD-based assays facilitate a rapid and quantitative serostatus profiling for the presence of anti-AAV antibodies

Novel assay format for total anti-adeno-associated virus antibody detection with low capsid consumption and built-in specificity control

“…Based on these experiments, the authors determined an optimum rAAV2 spike concentration of 1.65 × 10 9 vp/ml (i.e., 8.25 × 10 7 vp/well). Thus, the IC assay demonstrated approximately 10- to 30-times lower capsid material consumption than the existing anti-AAV total antibody assays [ 12 , 13 , 19 ].…”

A sensitive AAV transduction inhibition assay assists evaluation of critical factors for detection and concordance of pre-existing antibodies

“…The AAV-binding TAb assay was reported to be universally applicable to all serotypes and used in pre-screening of non-human primates in animal studies and patients in clinic 33 ; however, appropriate characterization of the antibody detection reagents is required to ensure that the assay reliably detects at a minimum isotypes IgG and IgM to evaluate recent and longer time exposures. The clinical enrollment assay cutoff often needs to be established based on preclinical animal studies.…”

Immunoassays and Mass Spectrometry for Determination of Protein Concentrations in Genetically Modified Crops

“…Few publications address the use of MSD multiplex technology for protein expression in the agriculture biotechnology industry . Nevertheless, there has been widespread use of this technology in the pharmaceutical industry and in clinical research. − MSD technology is very sensitive, with ultralow pico-gram level detection limits and a wide dynamic range of five or more orders of magnitude . This is particularly useful when analyzing samples with multiple traits that express trait proteins at widely varying levels.…”

“…Overall, immunoassays are powerful bioanalytical methods for quantifying traits and endogenous proteins in GM crops. However, the relatively costly and time-consuming method development process for immunoassays, the potential crossreactivity with other proteins and multiplex assay reagents, 40 and the lower tolerance for the harsh extraction buffers required for some analyte proteins may limit their applicability for quantifying some proteins in agricultural biotechnology (Table 1). Therefore, MS technology is being implemented more often as an alternative approach for the quantification of proteins of GM crops.…”