MRI of Transgene Expression: Correlation to Therapeutic Gene Expression

Ichikawa, Tomotsugu; Högemann, Dagmar; Saeki, Yoshinaga; Tyminski, Edyta; Terada, Kinya; Weissleder, Ralph; Chiocca, E. Antonio; Basilion, James P.

doi:10.1038/sj.neo.7900266

Cited by 114 publications

(62 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…142 Numerous studies have shown that SPIO conjugated to transferrin can be used to monitor the expression and regulation of the transferrin receptor in vitro and in vivo. 60,62,77,94,115 In parallel to the work with transferrin, SPIO conjugated to folate experienced rapid and effcient uptake by tumor cells overexpressing the folate receptor. In vivo MR imaging revealed an average T2 intensity decrease of 38% from pre-contrast to post-contrast images of the tumor.…”

Section: Cancer Imagingmentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

et al. 2006

View full text Add to dashboard Cite

Abstract-The field of molecular imaging has recently seen rapid advances in the development of novel contrast agents and the implementation of insightful approaches to monitor biological processes non-invasively. In particular, superparamagnetic iron oxide nanoparticles (SPIO) have demonstrated their utility as an important tool for enhancing magnetic resonance contrast, allowing researchers to monitor not only anatomical changes, but physiological and molecular changes as well. Applications have ranged from detecting inflammatory diseases via the accumulation of non-targeted SPIO in infiltrating macrophages to the specific identification of cell surface markers expressed on tumors. In this article, we attempt to illustrate the broad utility of SPIO in molecular imaging, including some of the recent developments, such as the transformation of SPIO into an activatable probe termed the magnetic relaxation switch.

show abstract

Section: Cancer Imagingmentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

et al. 2006

View full text Add to dashboard Cite

show abstract

“…The pellet was resuspended in DMEM with 20% FBS, 1% P/S and 1% amphotericin at 371C in a 5% CO 2 /95% air atmosphere. Human Gli36DEGFR glioma cells (kind gift of Dr David Louis, Molecular Neurooncology Laboratory, MGH, Boston, MA, USA; Ichikawa et al 58 ) and BHK-21 (ATCC, Rockville, MD, USA) were grown in DMEM containing 10% FBS and 1% P/S at 371C and 5% CO 2 /95% air atmosphere.…”

Section: Cell Culturementioning

confidence: 99%

Variability in infectivity of primary cell cultures of human brain tumors with HSV-1 amplicon vectors

et al. 2005

View full text Add to dashboard Cite

We investigated the variability in infectivity of cells in primary brain tumor samples from different patients using an HSV-1 amplicon vector. We studied the infectivity of HSV-1 amplicon vectors in tumor samples derived from neurosurgical resections of 20 patients. Cells were infected with a definite amount of HSV-1 amplicon vector HSV-GFP. Transduction efficiency in primary tumor cell cultures was compared to an established human glioma line. Moreover, duration of transgene expression was monitored in different tumor cell types. All primary cell cultures were infectable with HSV-GFP with variable transduction efficiencies ranging between 3.0 and 42.4% from reference human Gli36DEGFR glioma cells. Transduction efficiency was significantly greater in anaplastic gliomas and meningiomas (26.7717.4%) compared to more malignant tumor types (glioblastomas, metastases; 11.278.5%; P ¼ 0.05). To further investigate the possible underlying mechanism of this variability, nectin-1/HevC expression was analyzed and was found to contribute, at least in part, to this variability in infectability. The tumor cells expressed the exogenous gene for 7 to 61 days with significant shorter expression in glioblastomas (18713 d) compared to anaplastic gliomas (42724 d; Po0.05). Interindividual variability of infectivity by HSV-1 virions might explain, at least in part, why some patients enrolled in gene therapy for glioblastoma in the past exhibited a sustained response to HSV-1-based gene-and virus therapy. Infectivity of primary tumor samples from respective patients should be tested to enable the development of efficient and safe herpes vector-based gene and virus therapy for clinical application. Gene Therapy (2005) 12, 588-596.

show abstract

“…therapeutic gene [38,361]. Currently, reporter genes associated with optical imaging are most popular (e.g., BLI of luciferase [38,362] and fluorescent imaging of GFP and longer wavelength variants [45], since they are cheap modalities, and highly sensitive results are rapidly available.…”

Section: Enzyme Reportersmentioning

confidence: 99%

VatuximabTM: Optimizing Therapeutic Strategies for Prostate Cancer Based on Dynamic MR Tumor Oximetry

Mason¹

2007

View full text Add to dashboard Cite

Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing this collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Department of Defense, Washington Headquarters Services, Directorate for Information Operations and Reports (0704-0188), 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS.

show abstract

MRI of Transgene Expression: Correlation to Therapeutic Gene Expression

Cited by 114 publications

References 33 publications

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

Variability in infectivity of primary cell cultures of human brain tumors with HSV-1 amplicon vectors

VatuximabTM: Optimizing Therapeutic Strategies for Prostate Cancer Based on Dynamic MR Tumor Oximetry

Contact Info

Product

Resources

About