MRI assessment of blood outgrowth endothelial cell homing using cationic magnetoliposomes

Soenen, Stefaan J.; Meyer, Simon F. De; Dresselaers, Tom; Velde, Greetje Vande; Pareyn, Inge; Braeckmans, Kevin; Cuyper, Marcel De; Himmelreich, Uwe; Vanhoorelbeke, Karen

doi:10.1016/j.biomaterials.2011.02.037

Cited by 24 publications

(15 citation statements)

References 49 publications

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“…PIs labeled with MLs showed a hypointense signal until day four, whereas PIs labeled with Endorem or Resovist (+PLL) did not show any hypointense signals by day four. The higher MRI sensitivity of MLs can be explained by intracellular aggregation caused after partial degradation of the outer lipid layer in the lysosomal environment [ 31 , 32 ].…”

Section: Resultsmentioning

confidence: 99%

Improved Labeling of Pancreatic Islets Using Cationic Magnetoliposomes

Ribeiro

Ketkar-Atre

Yin

et al. 2018

JPM

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Pancreatic islets (PIs) transplantation is an alternative approach for the treatment of severe forms of type 1 diabetes (T1D). To monitor the success of transplantation, it is desirable to follow the location of engrafted PIs non-invasively. In vivo magnetic resonance imaging (MRI) of transplanted PIs is a feasible cell tracking method; however, this requires labeling with a suitable contrast agent prior to transplantation. We have tested the feasibility of cationic magnetoliposomes (MLs), compared to commercial contrast agents (Endorem and Resovist), by labeling insulinoma cells and freshly isolated rat PIs. It was possible to incorporate Magnetic Ressonance (MR)-detectable amounts of MLs in a shorter time (4 h) when compared to Endorem and Resovist. MLs did not show negative effects on the PIs’ viability and functional parameters in vitro. Labeled islets were transplanted in the renal sub-capsular region of healthy mice. Hypointense contrast in MR images due to the labeled PIs was detected in vivo upon transplantation, while MR detection of PIs labeled with Endorem and Resovist was only possible after the addition of transfection agents. These findings indicate that MLs are suitable to image PIs, without affecting their function, which is promising for future longitudinal pre-clinical and clinical studies involving the assessment of PI transplantation.

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Section: Resultsmentioning

confidence: 99%

Improved Labeling of Pancreatic Islets Using Cationic Magnetoliposomes

Ribeiro

Ketkar-Atre

Yin

et al. 2018

JPM

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“…MRI data acquisition and processing was similar to previous reports [44]. In brief, in vivo MRI was performed using a 9.4 T small animal MRI system (BioSpec, Bruker Biospin, Ettlingen, Germany) equipped with a gradient insert with a maximum gradient strength of 600 mT m −1 .…”

Section: Magnetic Resonance Imaging (Mri)mentioning

confidence: 99%

Assessment of the Theranostic Potential of Gold Nanostars—A Multimodal Imaging and Photothermal Treatment Study

et al. 2020

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Gold nanoparticles offer the possibility to combine both imaging and therapy of otherwise difficult to treat tumors. To validate and further improve their potential, we describe the use of gold nanostars that were functionalized with a polyethyleneglycol-maleimide coating for in vitro and in vivo photoacoustic imaging (PAI), computed tomography (CT), as well as photothermal therapy (PTT) of cancer cells and tumor masses, respectively. Nanostar shaped particles show a high absorption coefficient in the near infrared region and have a hydrodynamic size in biological medium around 100 nm, which allows optimal intra-tumoral retention. Using these nanostars for in vitro labeling of tumor cells, high intracellular nanostar concentrations could be achieved, resulting in high PAI and CT contrast and effective PTT. By injecting the nanostars intratumorally, high contrast could be generated in vivo using PAI and CT, which allowed successful multi-modal tumor imaging. PTT was successfully induced, resulting in tumor cell death and subsequent inhibition of tumor growth. Therefore, gold nanostars are versatile theranostic agents for tumor therapy.

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“…30 In brief, in vivo MRI was performed using a 9.4 T small animal MRI system (BioSpec, Bruker Biospin, Ettlingen, Germany) equipped with a gradient insert and a maximum gradient strength of 600 mT/m. A quadrature transmit-receive coil (Bruker Biospin) with an inner diameter of 7 cm was used for data acquisition.…”

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confidence: 99%

Development of nanostars as a biocompatible tumor contrast agent: toward in vivo SERS imaging

D’Hollander¹,

Mathieu²,

Jans³

et al. 2016

IJN

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The need for sensitive imaging techniques to detect tumor cells is an important issue in cancer diagnosis and therapy. Surface-enhanced Raman scattering (SERS), realized by chemisorption of compounds suitable for Raman spectroscopy onto gold nanoparticles, is a new method for detecting a tumor. As a proof of concept, we studied the use of biocompatible gold nanostars as sensitive SERS contrast agents targeting an ovarian cancer cell line (SKOV3). Due to a high intracellular uptake of gold nanostars after 6 hours of exposure, they could be detected and located with SERS. Using these nanostars for passive targeting after systemic injection in a xenograft mouse model, a detectable signal was measured in the tumor and liver in vivo. These signals were confirmed by ex vivo SERS measurements and darkfield microscopy. In this study, we established SERS nanostars as a highly sensitive contrast agent for tumor detection, which opens the potential for their use as a theranostic agent against cancer.

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MRI assessment of blood outgrowth endothelial cell homing using cationic magnetoliposomes

Cited by 24 publications

References 49 publications

Improved Labeling of Pancreatic Islets Using Cationic Magnetoliposomes

Improved Labeling of Pancreatic Islets Using Cationic Magnetoliposomes

Assessment of the Theranostic Potential of Gold Nanostars—A Multimodal Imaging and Photothermal Treatment Study

Development of nanostars as a biocompatible tumor contrast agent: toward in vivo SERS imaging

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