Movement of Water in Conjunction with Plant Movement Visualized by NMR Imaging1

Tamiya, Tôru; Miyazaki, Toshiyuki; Ishikawa, Hiroshi; Iriguchi, Norio; Maki, Takeshi; Matsumoto, Juichiro J.; Tsuchiya, Takahide

doi:10.1093/oxfordjournals.jbchem.a122421

Cited by 59 publications

(56 citation statements)

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“…One of the most remarkable results is the immediate disappearance of water in the lower part of the pulvinus as it leans down25. This result implies that the M. pudica motion is deeply related to water transport.…”

Section: Resultsmentioning

confidence: 94%

Real-time imaging of pulvinus bending in Mimosa pudica

Song

Yeom

Lee

2014

Sci Rep

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Mimosa pudica is a plant that rapidly shrinks its body in response to external stimuli. M. pudica does not perform merely simple movements, but exhibits a variety of movements that quickly change depending on the type of stimuli. Previous studies have investigated the motile mechanism of the plants from a biochemical perspective. However, an interdisciplinary study on the structural characteristics of M. pudica should be accompanied by biophysical research to explain the principles underlying such movements. In this study, the structural characteristics and seismonastic reactions of M. pudica were experimentally investigated using advanced bio-imaging techniques. The results show that the key factors for the flexible movements by the pulvinus are the following: bendable xylem bundle, expandable/shrinkable epidermis, tiny wrinkles for surface modification, and a xylem vessel network for efficient water transport. This study provides new insight for better understanding the M. pudica motile mechanism through structural modification.

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Section: Resultsmentioning

confidence: 94%

Real-time imaging of pulvinus bending in Mimosa pudica

Song

Yeom

Lee

2014

Sci Rep

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“…This movement is caused by a loss of turgor pressure in one half of the pulvinus (extensor [7]), which is located at the base of each primary petiole, pinna, and pinnule (leaflet) [6]. Nuclear magnetic resonance (NMR) imaging demonstrated that water is translocated from the extensor half to the other half (flexor [7]) of the pulvinus during the movement [8]. At the cellular level, individual “motor cells” in the extensor half of the pulvinus shrink following outflow of intracellular water [9], [10], which is accompanied by a large efflux of K + and Cl - ions [11], [12], [13], [14].…”

Section: Introductionmentioning

confidence: 99%

Development of an Agrobacterium-Mediated Stable Transformation Method for the Sensitive Plant Mimosa pudica

et al. 2014

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The sensitive plant Mimosa pudica has long attracted the interest of researchers due to its spectacular leaf movements in response to touch or other external stimuli. Although various aspects of this seismonastic movement have been elucidated by histological, physiological, biochemical, and behavioral approaches, the lack of reverse genetic tools has hampered the investigation of molecular mechanisms involved in these processes. To overcome this obstacle, we developed an efficient genetic transformation method for M. pudica mediated by Agrobacterium tumefaciens (Agrobacterium). We found that the cotyledonary node explant is suitable for Agrobacterium-mediated transformation because of its high frequency of shoot formation, which was most efficiently induced on medium containing 0.5 µg/ml of a synthetic cytokinin, 6-benzylaminopurine (BAP). Transformation efficiency of cotyledonary node cells was improved from almost 0 to 30.8 positive signals arising from the intron-sGFP reporter gene by using Agrobacterium carrying a super-binary vector pSB111 and stabilizing the pH of the co-cultivation medium with 2-(N-morpholino)ethanesulfonic acid (MES) buffer. Furthermore, treatment of the explants with the detergent Silwet L-77 prior to co-cultivation led to a two-fold increase in the number of transformed shoot buds. Rooting of the regenerated shoots was efficiently induced by cultivation on irrigated vermiculite. The entire procedure for generating transgenic plants achieved a transformation frequency of 18.8%, which is comparable to frequencies obtained for other recalcitrant legumes, such as soybean (Glycine max) and pea (Pisum sativum). The transgene was stably integrated into the host genome and was inherited across generations, without affecting the seismonastic or nyctinastic movements of the plants. This transformation method thus provides an effective genetic tool for studying genes involved in M. pudica movements.

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“…because of groups of antagonistic specialized cells that are located in 'hinges' called pulvini. Upon stimulation, the cells on one side of the pulvinus swell and those on the other side shrink, thereby actuating the movement (Tamiya et al 1988).…”

Section: Introductionmentioning

confidence: 99%

At the conjunction of biology, chemistry and physics: the fast movements ofDionaea, Aldrovanda, UtriculariaandStylidium

Joyeux¹

2011

Frontiers in Life Science

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Plants and fungi are able to move to perform essential functions. When these functions rely on insects, e.g. the nutrition uptake of carnivorous plants such as Dionaea, Aldrovanda, and Utricularia or the cross-pollination mechanism of Stylidium, then these movements must be both very rapid (faster than the second time scale) and repetitive. This paper discusses the different strategies these plants have developed to achieve this goal, and is aimed at pointing out how intricate the involved biological, chemical and physical mechanisms are. Before presenting some recent advances in the modelling of these plants, the paper first describes the functioning of motor cells, which is based on the equilibration of osmotic pressures, as well as the electric signalling through the propagation of action potentials, by which the stimulation due to the insect is transmitted to these motor cells. The paper then discusses the mechanisms these plants use to perform movements well below the second time scale, which are essentially based on the physical phenomenon known as snap-buckling in material sciences. The Conclusions mention several points that are not well understood and would clearly deserve further attention.

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Movement of Water in Conjunction with Plant Movement Visualized by NMR Imaging1

Cited by 59 publications

References 0 publications

Real-time imaging of pulvinus bending in Mimosa pudica

Real-time imaging of pulvinus bending in Mimosa pudica

Development of an Agrobacterium-Mediated Stable Transformation Method for the Sensitive Plant Mimosa pudica

At the conjunction of biology, chemistry and physics: the fast movements ofDionaea, Aldrovanda, UtriculariaandStylidium

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