Mouse T-cell receptor variable gene segment families

Abstract

All mouse T-cell receptor cz/8, ~3, and y variable (Tcra/d-, b-, and g-V) gene segments were aligned to compare the sequences with one another, to group them into subfamilies, and to derive a name which complies with the standard nomenclature. It was necessary to change the names of some V gene segments because they conflicted with those of other segments. The traditional classification into subfamilies was re-evaluated using a much larger pool of sequences. In the mouse, most V gene segments can be grouped into subfamilies of closely related genes with significantly less similarity between different subfamilies.

Anatomy of T cell autoimmunity to myelin oligodendrocyte glycoprotein (MOG): Prime role of MOG44F in selection and control of MOG‐reactive T cells in H‐2b mice

“…1C, MOG40-48 stimulated MOG37-52-reactive line T cells weakly, albeit significantly, whereas MOG42-50 was consistently non-stimulatory. Similarly, MOG39-48, encompassing MOG40-48 with a single amino acid addition at the N-terminus, could stimulate MOG37-52-specific line T cells, whereas MOG41-52, its counterpart Assigned by FACS with available mAb and/or PCR; nomenclature according to Arden et al [20] c) for the potential core sequence MOG42-50, had no effect (Fig. 1C).…”

“…Twenty T cell clones were isolated from encephalitogenic MOG35-55-specific line T cells (96% CD4 + , data not shown); they showed diverse TCR expression, utilizing eight different Vb and six different Va gene families (Table 1; V gene nomenclature according to [20]). The majority of the clones (clones 3, 5, 6, 3a, 4a, 5a, 10a, 12a, 14a and 15a) expressed Vb8, an observation on par with the predominant expression of Vb8 by MOG35-55-specific T cell lines from H-2 b mice (48%; [8,9]).…”

Developmental Stage-Specific Regulation of TCR-α-Chain Gene Assembly by Intrinsic Features of the TEA Promoter

“…The TCR␣/␦ locus spans ϳ1.6 Mb with the V␣ and V␦ gene segments clustered over 1.5 Mb in the 5Ј region of the locus ( Fig. 1) (9,10). Some V gene segments are used primarily in TCR␣-chain gene assembly (V␣ gene segments), and others primarily in TCR␦-chain gene assembly (V␦ gene segments).…”

“…Some V gene segments are used primarily in TCR␣-chain gene assembly (V␣ gene segments), and others primarily in TCR␦-chain gene assembly (V␦ gene segments). However, most V gene segments are used in both TCR␣-and TCR␦-chain gene assembly (V␣/␦ gene segments) (10). The V gene segment cluster is followed by two D␦ (D␦1 and D␦2) gene segments, two J␦ (J␦1 and J␦2) gene segments, and the TCR␦ constant region gene (C␦) (Fig.…”

Development of Dendritic Epidermal T Cells with a Skewed Diversity of γδTCRs in Vδ1-Deficient Mice

“…The first wave appearing around gestation day (GD) 4 14 -16 expresses a canonical V␥5 (GV1 by World Health Organization-International Union of Immunological Sciences nomenclature, see Ref. 6)-J␥1-C␥1 chain, and the second wave appearing around GD16 -18 expresses a canonical V␥6 (GV2)-J␥1-C␥1 chain. Both the V␥5 and V␥6 chains preferentially pair with a common canonical V␦1 (DV101)-D␦2-J␦2-C␦ chain (5,(7)(8)(9).…”