Mouse sperm K+ currents stimulated by pH and cAMP possibly coded by Slo3 channels

Martínez-López, Pablo; Santi, Celia M.; Treviño, Claudia L.; Ocampo-Gutiérrez, Ana Y; Acevedo-Fernández, Juan José; Alisio, Arturo; Salkoff, Lawrence; Darszon, Alberto

doi:10.1016/j.bbrc.2009.02.008

Cited by 58 publications

(73 citation statements)

References 17 publications

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“…When expressed in Xenopus oocytes, SLO3 is also stimulated by elevated cAMP levels through a PKA-dependent phosphorylation. Patch-clamp recordings in testicular mouse spermatozoa revealed that a K ϩ current that is time and voltage dependent is activated by intracellular alkalinization, has a P K /P Na Ն5, is weakly blocked by TEA, and is very sensitive to Ba 2ϩ (346). These currents are absent in testicular spermatozoa from Slo3 null mice, confirming their identity.…”

Section: F Capacitationmentioning

confidence: 78%

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Calcium Channels in the Development, Maturation, and Function of Spermatozoa

Darszon

Nishigaki

Beltrán

et al. 2011

Physiological Reviews

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314

313

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-permeable channels and located them at distinct sites in spermatozoa so that they can help fertilize the egg. New tools to study sperm ionic currents, and image intracellular Ca 2ϩ with better spatial and temporal resolution even in swimming spermatozoa, are revealing how sperm ion channels participate in fertilization. This review critically examines the involvement of Ca 2ϩ channels in multiple signaling processes needed for spermatozoa to mature, travel towards the egg, and fertilize it. Remarkably, these tiny specialized cells can express exclusive channels like CatSper for Ca 2ϩ and SLO3 for K ϩ , which are attractive targets for contraception and for the discovery of novel signaling complexes. Learning more about fertilization is a matter of capital importance; societies face growing pressure to counteract rising male infertility rates, provide safe male gamete-based contraceptives, and preserve biodiversity through improved captive breeding and assisted conception initiatives.

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Section: F Capacitationmentioning

confidence: 78%

“…329,433). Since then, several ion channels have been detected in testicular and epididymal mouse spermatozoa (137,346,381,431,450,566). Intriguingly, Ca V 3 currents such as those recorded in spermatogenic cells were detected in testicular but not in epididymal sperm (137,566).…”

Section: Ca V Channels In Spermatogenic Cells and Spermatozoamentioning

confidence: 99%

Calcium Channels in the Development, Maturation, and Function of Spermatozoa

Darszon

Nishigaki

Beltrán

et al. 2011

Physiological Reviews

Self Cite

314

313

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“…Sperm were harvested from the seminiferous tubules of adult CD1 mice as previously described (28) and whole-cell currents in patch-clamped sperm using established methods (53). We used testicular sperm to avoid prior exposure of cells to endogenous (epididymal) CRISP4.…”

Section: Methodsmentioning

confidence: 99%

Cysteine-rich secretory protein 4 is an inhibitor of transient receptor potential M8 with a role in establishing sperm function

Gibbs¹,

Orta

Reddy³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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110

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The cysteine-rich secretory proteins (CRISPs) are a group of four proteins in the mouse that are expressed abundantly in the male reproductive tract, and to a lesser extent in other tissues. Analysis of reptile CRISPs and mouse CRISP2 has shown that CRISPs can regulate cellular homeostasis via ion channels. With the exception of the ability of CRISP2 to regulate ryanodine receptors, the in vivo targets of mammalian CRISPs function are unknown. In this study, we have characterized the ion channel regulatory activity of epididymal CRISP4 using electrophysiology, cell assays, and mouse models. Through patch-clamping of testicular sperm, the CRISP4 CRISP domain was shown to inhibit the transient receptor potential (TRP) ion channel TRPM8. These data were confirmed using a stably transfected CHO cell line. TRPM8 is a major cold receptor in the body, but is found in other tissues, including the testis and on the tail and head of mouse and human sperm. Functional assays using sperm from wild-type mice showed that TRPM8 activation significantly reduced the number of sperm undergoing the progesteroneinduced acrosome reaction following capacitation, and that this response was reversed by the coaddition of CRISP4. In accordance, sperm from Crisp4 null mice had a compromised ability to undergo to the progesterone-induced acrosome reaction. Collectively, these data identify CRISP4 as an endogenous regulator of TRPM8 with a role in normal sperm function.cysteine-rich secretory proteins | antigen 5 | pathogenesis-related 1 | epididymal maturation | fertility

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“…The activation of insect sperm via ionotropic Ors is reminiscent of capacitation of mammalian sperm, which has been linked to signaling pathways mediated by metabotropic ORs (23,26,27) as well as the activity of several ionotropic channels (58,59). These include calcium channels, most notably the sperm cation channels, CatSpers (60)(61)(62)(63), potassium channels such as Kcnu1 (64,65) as well as sodium, proton, bicarbonate, and chloride channels that are localized along the sperm flagella and speculated to act downstream of receptors for diverse extracellular ligands (33,59). The modulation of insect and mammalian sperm via proteins of distinct evolutionary origins yet that encompass conserved modes of signal transduction represents a potent example of convergent evolution impacting upon a singularly essential biological process.…”

Section: Significancementioning

confidence: 99%

Odorant receptor-mediated sperm activation in disease vector mosquitoes

Pitts

Liu

Zhou

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Insects, such as the malaria vector mosquito, Anopheles gambiae, depend upon chemoreceptors to respond to volatiles emitted from a range of environmental sources, most notably blood meal hosts and oviposition sites. A subset of peripheral signaling pathways involved in these insect chemosensory-dependent behaviors requires the activity of heteromeric odorant receptor (OR) ion channel complexes and ligands for numerous A. gambiae ORs (AgOrs) have been identified. Although AgOrs are expressed in nonhead appendages, studies characterizing potential AgOr function in nonolfactory tissues have not been conducted. In the present study, we explore the possibility that AgOrs mediate responses of spermatozoa to endogenous signaling molecules in A. gambiae. In addition to finding AgOr transcript expression in testes, we show that the OR coreceptor, AgOrco, is localized to the flagella of A. gambiae spermatozoa where Orco-specific agonists, antagonists, and other odorant ligands robustly activate flagella beating in an Orco-dependent process. We also demonstrate Orco expression and Orco-mediated activation of spermatozoa in the yellow fever mosquito, Aedes aegypti. Moreover, we find Orco localization in testes across distinct insect taxa and posit that OR-mediated responses in spermatozoa may represent a general characteristic of insect reproduction and an example of convergent evolution.transcriptomics | cell signaling | gamete | chemical ecology | Culicidae

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Mouse sperm K+ currents stimulated by pH and cAMP possibly coded by Slo3 channels

Cited by 58 publications

References 17 publications

Calcium Channels in the Development, Maturation, and Function of Spermatozoa

Calcium Channels in the Development, Maturation, and Function of Spermatozoa

Cysteine-rich secretory protein 4 is an inhibitor of transient receptor potential M8 with a role in establishing sperm function

Odorant receptor-mediated sperm activation in disease vector mosquitoes

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