Mouse Mos protooncogene product is present and functions during oogenesis.

Paules, Richard S.; Buccione, Roberto; Moschel, Robert C.; Woude, George F. Vande; Eppig, John J.

doi:10.1073/pnas.86.14.5395

Cited by 157 publications

(95 citation statements)

References 54 publications

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“…In the case of other proteins that accumulate during oocyte maturation, including tissue-type plasminogen activator [tPA (Huarte et al, 1987)], HPRT (Paynton et al, 1988), mos (O'Keefe et al, 1989;Paules et al, 1989;Gebauer et al, 1994), FGF receptor (Culp and Musci, 1999) cyclin B (Polanski et al, 1998;Barkoff et al, 2000;Tay et al, 2000) and spindlin (Oh et al, 2000), this is due to increased translation of existing mRNAs. Translation of these mRNAs is regulated by U-rich sequences, termed adenylation control elements (ACE) or cytoplasmic polyadenylation elements (CPE), that are located in the 3′-untranslated region (utr) of the mRNA within about 100 nt of the polyadenylation signal (Richter, 1995;Gray and Wickens, 1998;Oh et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Stem-loop binding protein accumulates during oocyte maturation and is not cell-cycle-regulated in the early mouse embryo

Allard

Champigny

Skoggard

et al. 2002

Journal of Cell Science

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The stem-loop binding protein (SLBP) binds to the 3′ end of histone mRNA and participates in 3′-processing of the newly synthesized transcripts, which protects them from degradation, and probably also promotes their translation. In proliferating cells, translation of SLBP mRNA begins at G1/S and the protein is degraded following DNA replication. These post-transcriptional mechanisms closely couple SLBP expression to S-phase of the cell cycle, and play a key role in restricting synthesis of replication-dependent histones to S-phase. In contrast to somatic cells,replication-dependent histone mRNAs accumulate and are translated independently of DNA replication in oocytes and early embryos. We report here that SLBP expression and activity also differ in mouse oocytes and early embryos compared with somatic cells. SLBP is present in oocytes that are arrested at prophase of G2/M, where it is concentrated in the nucleus. Upon entry into M-phase of meiotic maturation, SLBP begins to accumulate rapidly,reaching a very high level in mature oocytes arrested at metaphase II. Following fertilization, SLBP remains abundant in the nucleus and the cytoplasm throughout the first cell cycle, including both G1 and G2 phases. It declines during the second and third cell cycles, reaching a relatively low level by the late 4-cell stage. SLBP can bind the histone mRNA-stem-loop at all stages of the cell cycle in oocytes and early embryos, and it is the only stem-loop binding activity detectable in these cells. We also report that SLBP becomes phosphorylated rapidly following entry into M-phase of meiotic maturation through a mechanism that is sensitive to roscovitine, an inhibitor of cyclin-dependent kinases. SLBP is rapidly dephosphorylated following fertilization or parthenogenetic activation, and becomes newly phosphorylated at M-phase of mitosis. Phosphorylation does not affect its stem-loop binding activity. These results establish that, in contrast to Xenopus, mouse oocytes and embryos contain a single SLBP. Expression of SLBP is uncoupled from S-phase in oocytes and early embryos, which indicates that the mechanisms that impose cell-cycle-regulated expression of SLBP in somatic cells do not operate in oocytes or during the first embryonic cell cycle. This distinctive pattern of SLBP expression may be required for accumulation of histone proteins required for sperm chromatin remodelling and assembly of newly synthesized embryonic DNA into chromatin.

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Section: Discussionmentioning

confidence: 99%

Stem-loop binding protein accumulates during oocyte maturation and is not cell-cycle-regulated in the early mouse embryo

Allard

Champigny

Skoggard

et al. 2002

Journal of Cell Science

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“…Tg⅐AC43 (a TPA-induced Tg⅐AC squamous cell carcinoma cell line) and FVB/N217 (an FVB/N carcinoma cell line) (43) were cultured in RPMI1640/Dulbecco's modified Eagle's medium (1:1) with 20% FBS. NIH/3T3 cells were maintained as described previously (44). Cell lines used in this study were free of mycoplasma infection.…”

Section: Cell Culturesmentioning

confidence: 99%

12-O-Tetradecanoylphorbol-13-acetate and UV Radiation-induced Nucleoside Diphosphate Protein Kinase B Mediates Neoplastic Transformation of Epidermal Cells

Wei

Trempus

Ali

et al. 2004

Journal of Biological Chemistry

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“…Mouse fibroblast cells Rat-1 and monkey transformed kidney cells COS-1 were cultured in Dulbecco's modified Eagle's medium (Dulbecco's MEM) containing 10% FBS. NIH/3T3 cells were maintained as described previously (28). Cell lines used in this study were free of mycoplasma infection.…”

Section: Animals and Cell Culturementioning

confidence: 99%

Identification of Dss1 as a 12-O-Tetradecanoylphorbol-13-acetate-responsive Gene Expressed in Keratinocyte Progenitor Cells, with Possible Involvement in Early Skin Tumorigenesis

Wei¹,

Trempus²,

Cannon³

et al. 2003

Journal of Biological Chemistry

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This study identifies genes expressed early in 12-Otetradecanoylphorbol-13-acetate (TPA)-induced skin carcinogenesis in genetically initiated Tg⅐AC v-Ha-ras transgenic mice. Keratinocyte progenitor cells from TPA-treated Tg⅐AC mice were isolated with fluorescence-activated cell sorting and expression was analyzed using cDNA microarray technology. Eleven genes were identified whose expression changed significantly in response to carcinogen treatment. Deleted in split hand/split foot 1 (Dss1) is a gene associated with a heterogeneous limb developmental disorder called split hand/split foot malformation. cDNA microarray expression analysis showed that the mouse homologue of Dss1 is induced by TPA. Dss1 overexpression was detected by Northern blot analysis in early TPA-treated hyperplastic skins and in JB6 Cl 41-5a epidermal cells. Interestingly, Dss1 expression was also shown to be elevated in skin papillomas relative to normal skins, and further increased in squamous cell malignancies. Functional studies by ectopically constitutive expression of Dss1 in JB6 Cl 41-5a preneoplastic cells strongly increased focus formation and proliferation of these cells and enhanced efficiency of neoplastic transformation of the cells in soft agar. These results strongly suggest that Dss1 is a TPA-inducible gene that may play an important role in the early stages of skin carcinogenesis.Skin carcinogenesis is a complex multistage process that progresses through distinct stages of initiation, promotion, progression, and malignancy (1-3). The Tg⅐AC mouse is a genetically modified (transgenic) form of the FVB/N mouse strain that carries a genomic copy of the v-Ha-ras gene fused to a fetal -globin gene promoter (4). Tg⅐AC mice have already entered the initiation stage of cancer development and have a higher sensitivity to many types of environmentally inducible cancer than wild type mice. Tg⅐AC mice develop hyperplasia in skin keratinocytes after exposure to tumor promoters such as TPA 1 (4), full thickness wounding (5), ultraviolet radiation (6), or carcinogens such as 7,12-dimethylbenz[a]anthracene (7). These hyperplasias eventually develop into benign papillomas, some of which become malignant tumors such as squamous cell carcinomas or spindle cell tumors (7). The in vivo Tg⅐AC mouse model is a valuable tool to study the early stages of skin carcinogenesis.The epidermis is a stratified, rapidly renewing tissue in which terminally differentiated cells are continuously lost from the skin surface and replaced by an intricate and highly regulated proliferative process. Skin cells are regenerated through the proliferative capacity of keratinocyte stem cells (KSCs) and transit amplifying (TA) cells in the basal layer. KSCs are a minor subpopulation of relatively quiescent cells that have broad proliferative potential and an unlimited capacity for self-renewal (8, 9). It has been proposed that carcinogens generate mutations in the population of stem cells which are transformed them into initiated preneoplastic cells (10). It has also been r...

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Mouse Mos protooncogene product is present and functions during oogenesis.

Cited by 157 publications

References 54 publications

Stem-loop binding protein accumulates during oocyte maturation and is not cell-cycle-regulated in the early mouse embryo

Stem-loop binding protein accumulates during oocyte maturation and is not cell-cycle-regulated in the early mouse embryo

12-O-Tetradecanoylphorbol-13-acetate and UV Radiation-induced Nucleoside Diphosphate Protein Kinase B Mediates Neoplastic Transformation of Epidermal Cells

Identification of Dss1 as a 12-O-Tetradecanoylphorbol-13-acetate-responsive Gene Expressed in Keratinocyte Progenitor Cells, with Possible Involvement in Early Skin Tumorigenesis

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