Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

1
22
0

Year Published

2007
2007
2010
2010

Publication Types

Select...
5
1
1

Relationship

2
5

Authors

Journals

citations
Cited by 57 publications
(23 citation statements)
references
References 5 publications
1
22
0
Order By: Relevance
“…In this study, the solvent control mutant frequencies of 93% of the experiments fell within those values. The TK mutation frequency in the WTK-1 cell solvent control was similar to the spontaneous mutation frequency reported for L5178Y cells [Moore et al, 2007]. Because both WTK-1 and L5178Y cells contain mutant p53 gene expressing mutant p53 protein, the high TK mutation frequencies are likely to be due to the mutator phenotype of the p53-deficient cells.…”
Section: Thymidine Kinase Gene Mutation Assaysupporting
confidence: 76%
See 1 more Smart Citation
“…In this study, the solvent control mutant frequencies of 93% of the experiments fell within those values. The TK mutation frequency in the WTK-1 cell solvent control was similar to the spontaneous mutation frequency reported for L5178Y cells [Moore et al, 2007]. Because both WTK-1 and L5178Y cells contain mutant p53 gene expressing mutant p53 protein, the high TK mutation frequencies are likely to be due to the mutator phenotype of the p53-deficient cells.…”
Section: Thymidine Kinase Gene Mutation Assaysupporting
confidence: 76%
“…Figure 1 shows the distribution of PE0 (survival) versus PE3 (viability) for the solvent control for TK6 cells (26 data points) and WTK-1 cells (27 data points). The mean PE (6 SD) was 93.0 6 18.0 (%) for TK6 cells and 85.56 17.6 (%) for WTK-1 cells and met the MLA acceptance criteria (65-120%) [Moore et al, 2007]. The mean 6 SD TK mutant frequency for the negative and positive control, respectively, was 4.96 6 2.30 (310 26 ) and 66.1 6 76.6 (310 26 ) for TK6 cells (26 experiments) and 162.4 6 64.0 (310 26 ) and 876.2 6 376.9 (310 26 ) for WTK-1cells (27 experiments) (Fig.…”
Section: Thymidine Kinase Gene Mutation Assaymentioning
confidence: 87%
“…ICH S2B also states that the detection of aneugens is enhanced by the use of a 24 hour treatment using the microwell method. The IWGT MLA Workgroup considered the usefulness of this requirement and published a consensus report on its fi ndings [27] . After analyzing 990 datasets, the group found that less than 2% of the chemicals were uniquely positive at 24 hours.…”
Section: Detailed Protocolsmentioning
confidence: 99%
“…The IWGT MLA Workgroup [27] also reached consensus on acceptance criteria for the soft agar and microwell method negative/vehicle control parameters for the 24 hour treatment, as shown in the following table. Suspension growth values were changed for the longer treatment time because it involves 3 days of suspension growth, rather than the 2 days for the 3 or 4 hour treatment.…”
Section: Criteria For Negative/vehicle and Positive Controlsmentioning
confidence: 99%
“…Thus, toxic TFT placed in the medium will be transported into normal Tk +/− (non-mutated) cells that consequently die, while Tk −/− mutants will be resistant to the toxic TFT and survive, and subsequently form clones that can be counted. The L5178Y system is the recommended in-vitro mammalian cell mutation assay because it detects a wide range of genetic alterations, including both mutations and chromosomal damage [18].…”
Section: Mammalian Mutagenicity Testingmentioning
confidence: 99%