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Cited by 26 publications
(5 citation statements)
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“…The existence of two different types of IgGl that exhibit differences in their reactivity in complement (Cl) fixation (9), and also a third type of IgGI which does not bind firmly to protein A at pH 8.0 was also reported. This third type is more prevalent with MAb (25). When the MAb were tested for reactivity with protein A at pH 7.0 and 8.0 through immunoblotting and autoradiography, faint bands were seen at pH 8.0 but not at pH 7.0.…”
Section: Resultsmentioning
confidence: 98%
“…The existence of two different types of IgGl that exhibit differences in their reactivity in complement (Cl) fixation (9), and also a third type of IgGI which does not bind firmly to protein A at pH 8.0 was also reported. This third type is more prevalent with MAb (25). When the MAb were tested for reactivity with protein A at pH 7.0 and 8.0 through immunoblotting and autoradiography, faint bands were seen at pH 8.0 but not at pH 7.0.…”
Section: Resultsmentioning
confidence: 98%
“…It should be noted that Table 1 shows the reactivity of these binding proteins with representative samples of the species and subclasses shown. This reactivity is not, however, absolute, and variations have been reported (Rousseaux et al, 1981;Langone, 1982a;Nilsson et al, 1983;Villemez et al ., 1984;Boyle and Reis, 1987). This is particularly relevant for mouse monoclonal antibodies, and it is not possible to predict with absolute certainty the reactivity of any class or subclass of monoclonal antibody with either protein A or protein G.…”
Section: Experimental Considerationsmentioning
confidence: 72%
“…This higher proportion of hybrid-hybrid may have been due to differential adsorption of mouse immunoglobulin subclasses on Protein A from only one cycle of ascitic fluid, resulting in a fraction enriched with the lgG1-lgG2a antibody. The weak binding of mouse IgGI to Protein A has been well documented (20,21,22).…”
Section: Discussionmentioning
confidence: 98%