Morphometry of boar sperm head and flagellum in semen backflow after insemination

García–Vázquez, Francisco Alberto; Caravaca, Iván Hernández; Yánez-Quintana, Wellington; Matás, Carmen; Soriano‐Úbeda, Cristina; Izquierdo-Rico, María José

doi:10.1016/j.theriogenology.2015.04.011

Cited by 20 publications

(15 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Differences in head size between fertile and infertile males have been detected in various species (Casey, Gravance, Davis, Chabot, & Liu, ). In a study by García‐Vázquez et al (), sperm analysed in the backflow had smaller heads, different head shapes and shorter tails than in sperm prior to insemination. Boars whose semen contained sperm with smaller heads have been found to be more fertile (Hirai et al, ), while the reverse has been observed in rabbits (Marco‐Jiménez et al, ).…”

Section: Discussionmentioning

confidence: 95%

“…Gómez Montoto et al () suggest that an increase in sperm velocity in rodents was associated with more elongated heads, which is also supported by research by Malo et al (). According to some authors (Fitzpatrick, Garcia‐Gonzalez, & Evans, ; Gomendio & Roldan, ), the shape of the sperm head affects its hydrodynamics in the female reproductive tract, and sperm with longer tails have a greater fertilization capacity due to increased motility (García‐Vázquez, Gadea, Matás, & Holt, ; García‐Vázquez et al, ; Partyka, Niżański, & Ochota, ). Assessment of the mitochondria and midpiece during semen analysis may therefore be useful as a marker of sperm fertility (Gallon, Marchetti, Jouy, & Marchetti, ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of the structure of chinchilla sperm isolated from semen and from the tail of the epididymis

Andraszek

Banaszewska

Szeleszczuk

et al. 2020

Reprod Domestic Animals

View full text Add to dashboard Cite

Sperm cells isolated from the tail of the epididymis and from the semen of the same individuals were analysed. The use of silver nitrate to stain sperm cells isolated from the tail of the epididymis made it possible to identify structures that were not visible in the sperm from semen. Silver nitrate very clearly distinguished the acrosomal and distal parts of the sperm head. Following silver nitrate staining, the sperm isolated from the tail of the epididymis were characterized by dark ‘collars’ in the distal part of the head. These ‘collars’ are not visible in the sperm cells isolated from semen. The results of the study indicate differences in the dimensions of sperm isolated from the tail of the epididymis and sperm in semen. Sperm isolated from the tail of the epididymis had smaller heads, despite their longer length, and had longer midpieces and tails than ejaculate sperm. Silver nitrate staining is a simple and fast technique. Silver nitrate makes it possible to identify the acrosome and post‐acrosomal region of the sperm head and to clearly identify the midpiece. Therefore, it can be successfully used to supplement routine techniques for evaluating sperm morphology or as an independent technique.

show abstract

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Comparison of the structure of chinchilla sperm isolated from semen and from the tail of the epididymis

Andraszek

Banaszewska

Szeleszczuk

et al. 2020

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…The first types of excluded spermatozoa are likely to be those with midpiece or tail defects that impair motility. Garcia-Vazquez et al 56. have shown that spermatozoa analyzed in the backflow were small (head and flagellum), with different head shapes compared with spermatozoa observed in the dose before insemination.…”

Section: Sperm Selection Within the Female Genital Tract Related To Mmentioning

confidence: 99%

Importance of sperm morphology during sperm transport and fertilization in mammals

et al. 2016

Self Cite

View full text Add to dashboard Cite

After natural or artificial insemination, the spermatozoon starts a journey from the site of deposition to the place of fertilization. However, only a small subset of the spermatozoa deposited achieves their goal: to reach and fertilize the egg. Factors involved in controlling sperm transport and fertilization include the female reproductive tract environment, cell-cell interactions, gene expression, and phenotypic sperm traits. Some of the significant determinants of fertilization are known (i.e., motility or DNA status), but many sperm traits are still indecipherable. One example is the influence of sperm dimensions and shape upon transport within the female genital tract towards the oocyte. Biophysical associations between sperm size and motility may influence the progression of spermatozoa through the female reproductive tract, but uncertainties remain concerning how sperm morphology influences the fertilization process, and whether only the sperm dimensions per se are involved. Moreover, such explanations do not allow the possibility that the female tract is capable of distinguishing fertile spermatozoa on the basis of their morphology, as seems to be the case with biochemical, molecular, and genetic properties. This review focuses on the influence of sperm size and shape in evolution and their putative role in sperm transport and selection within the uterus and the ability to fertilize the oocyte.

show abstract

“…However, it is not yet known in detail how these processes affect sperm morphology; more importantly, the morphometric characteristics of the cells that eventually fertilize the oocyte are unknown. Recently, García-Vázquez et al 130. reported how boar spermatozoa in the female reproductive tract are selected on the basis of their size and shape, with those with a larger head and longer tail being those reaching the fertilization site.…”

Section: Assessment Of Sperm Morphometry II – Functional Aspectsmentioning

confidence: 99%

Current status and potential of morphometric sperm analysis

et al. 2016

View full text Add to dashboard Cite

The spermatozoon is the most diverse cell type known and this diversity is considered to reflect differences in sperm function. How the diversity in sperm morphology arose during speciation and what role the different specializations play in sperm function, however, remain incompletely characterized. This work reviews the hypotheses proposed to explain sperm morphological evolution, with a focus on some aspects of sperm morphometric evaluation; the ability of morphometrics to predict sperm cryoresistance and male fertility is also discussed. For this, the evaluation of patterns of change of sperm head morphometry throughout a process, instead of the study of the morphometric characteristics of the sperm head at different stages, allows a better identification of the males with different sperm cryoconservation ability. These new approaches, together with more studies employing a greater number of individuals, are needed to obtain novel results concerning the role of sperm morphometry on sperm function. Future studies should aim at understanding the causes of sperm design diversity and the mechanisms that generate them, giving increased attention to other sperm structures besides the sperm head. The implementation of scientific and technological advances could benefit the simultaneous examination of sperm phenotype and sperm function, demonstrating that sperm morphometry could be a useful tool for sperm assessment.

show abstract

Morphometry of boar sperm head and flagellum in semen backflow after insemination

Cited by 20 publications

References 49 publications

Comparison of the structure of chinchilla sperm isolated from semen and from the tail of the epididymis

Comparison of the structure of chinchilla sperm isolated from semen and from the tail of the epididymis

Importance of sperm morphology during sperm transport and fertilization in mammals

Current status and potential of morphometric sperm analysis

Contact Info

Product

Resources

About