Morphometry and morphology of nucleus of the Sertoli and interstitial cells of the tambaqui Colossoma macropomum (Cuvier, 1881) (Pisces: Characidae) during the reproductive cycle

Abstract

This study allowed the characterization of the tambaqui Colossoma macropomum testes structural organization, emphasizing Sertoli and interstitial cells and analyzing morphometrically the Sertoli cell nucleus diameter and the interstitial tissue area during the reproductive cycle. Fragments of tambaqui testes were collected in the following reproductive cycle stages: immature, resting, maturation I and II, mature, and regression, and were histologically processed. The Sertoli cells were found at the periphery of the cysts of germinative lineage cells and the nuclei were shown to be smaller as these cells developed. The interstitial cells were better observed between the seminiferous lobules next to vessels in the interstitial tissue of maturing testes.

Seasonal Histology of the Testes of Nile Tilapia (Oreochromis niloticus)

“…The germinal cysts were surrounded with the cytoplasmic processes of the Sertoli cells that shared with very thin collagen fibers in formation of the wall of cysts. This resembled the results of Arenas et al, (1995) in Gambusia affinis and Nakaghi et al (2003) in Colossoma macropomum. Sertoli cells were also found surrounding the spermatogonia and this was supported by the results of Abd El-Aziz (1992) in Oreochromis niloticus.…”

“…Our result revealed that the Leydig (interstitial) cells were present in the intertubular spaces with numerous blood capillaries. This result was similar to those of Van Vuren and Soly (1990) in Tilapia rendalli; Mousa (1998) in Oreochromis niloticus; Nakaghi et al, (2003) in Colossoma macropomum and Smita et al, (2005) in Ichthyophis tricolor. Leydig cells were poly gonal in shape, with ill-definite cell boundaries and had centrally located spherical nuclei.…”

The Expression of Multiple Connexins Throughout Spermatogenesis in the Rainbow Trout Testis Suggests a Role for Complex Intercellular Communication1

“…Cx30 expression appeared to increase between stages 1 and 2 of spermatogenesis, which is consistent with the notion that gap junctional communication is involved in the control of androgen secretion [18,37,38]. This observation is consistent with RT-PCR data, which suggest an increase in cx30 mRNA levels between stages 1 and 2.…”

Comparative Morphological Studies on Testes and Seminal Vesicles of African Catfish (Clarias gariepinus) and Nile Tilapia (Tilapia nilotica)

“…This result of the current study which revealed that, the interstitial cells of Leydig which were present in the intertubular spaces with numerous blood capillaries in both species is similar to the reports of Wepener et al (2001) in Tilapia randalii (Arnold et al, 2000) in Oreochromis niloticus (Nakaghi et al, 2003) in Colossoma macropomum and (Smita et al, 2005) in Ichthyophis tricolor. The results of the present study on Leydig cells being polygonal in shape with ill-definite cell boundaries and centrally located spherical nuclei are in line with the result of Zakariah et al (2016a, b) and El-Sakhawy et al (2011) in Clarias gariepinus (Ahmed et al, 2013) in Clarias microcephalus, in Oreochromis niloticus.…”