Morphology of the kidney in larvae ofBufo viridis (Amphibia, Anura, Bufonidae)

Møbjerg, Nadja; Larsen, Erik Hviid; Jespersen, Åse

doi:10.1002/1097-4687(200009)245:3<177::aid-jmor1>3.0.co;2-f

Cited by 43 publications

(32 citation statements)

References 18 publications

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“…As is the case in fish, the segmental organization of the amphibian pronephros has only been recently appreciated and led to a revised model of its structure 8,9,[13][14][15][16][17][18] (see inset, Figure 1c). Distinct pronephros segment domains can be identified in amphibians based on their histological features.…”

Section: Pronephros Segmentation Is Conserved Among Lower Vertebratesmentioning

confidence: 99%

“…Distinct pronephros segment domains can be identified in amphibians based on their histological features. 13 Expression studies in the frog, Xenopus laevis, have shown that the tubule consists of at least two proximal segments and two distal segments, referred to as early and late, followed by a duct segment (Figure 1c). These five segments express solute transporters that liken them to the proximal and distal segments of both the zebrafish pronephros and the mammalian metanephros ( Figure 2).…”

Section: Pronephros Segmentation Is Conserved Among Lower Vertebratesmentioning

confidence: 99%

“…4,5 New studies in zebrafish, as well as frogs, have discovered that the nephrons of the embryonic (pronephric) kidneys have a segmental organization similar to that found in adult (metanephric) mammalian kidneys, thus uncovering conservation between nephrons from different kidney types. [6][7][8][9][10][11][12][13][14][15][16][17][18] These findings raise the exciting prospect of utilizing the benefits of lower vertebrate models to dissect the developmental pathways controlling nephron segmentation. This review will focus on the segment pattern of the zebrafish pronephros, how it compares to frogs and mammals, and how segmental identity is established during zebrafish nephron development.…”

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The zebrafish pronephros: A model to study nephron segmentation

Wingert

Davidson

2008

Kidney International

183

203

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Nephrons possess a segmental organization where each segment is specialized for the secretion and reabsorption of particular solutes. The developmental control of nephron segment patterning remains one of the enigmas within the field of renal biology. Achieving an understanding of the mechanisms that direct nephron segmentation has the potential to shed light on the causes of kidney birth defects and renal diseases in humans. Researchers studying embryonic kidney development in zebrafish and Xenopus have recently demonstrated that the pronephric nephrons in these vertebrates are segmented in a similar fashion as their mammalian counterparts. Further, it has been shown that retinoic acid signaling establishes proximodistal segment identities in the zebrafish pronephros by modulating the expression of renal transcription factors and components of signaling pathways that are known to direct segment fates during mammalian nephrogenesis. These findings present the zebrafish model as an excellent genetic system in which to interrogate the conserved developmental pathways that control nephron segmentation in both lower vertebrates and mammals.

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Section: Pronephros Segmentation Is Conserved Among Lower Vertebratesmentioning

confidence: 99%

Section: Pronephros Segmentation Is Conserved Among Lower Vertebratesmentioning

confidence: 99%

mentioning

confidence: 99%

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The zebrafish pronephros: A model to study nephron segmentation

Wingert

Davidson

2008

Kidney International

183

203

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“…We have previously reported that the epithelial sodium channel (ENaC) is localized in the terminal portion of the distal tubule in the pronephros by immunohistochemistry (Kumano et al 2005). On the other hand, vacuolar-type H + -ATPase and intercalated cells are absent from the terminal portion of the distal tubule of the pronephros (Møbjerg et al 2000;M. Uchiyama and H. Yoshizawa, unpublished).…”

Section: Nhe3 Expression During Ontogenymentioning

confidence: 95%

“…In the present study, positive staining for NHE3 has not been observed in the proximal tubules but has been detected in the early distal nephron, such as the diluting segments, and the initial part of late distal tubules in H. nigrescens. Ultrastructural and immunohistochemical studies have revealed that, although well-developed interdigitations of the basolateral membrane and prominent numbers of mitochondria are present along the mammalian proximal tubule, these structures are absent, and the presence of Na + , K + -ATPase is sparse, in the proximal tubule cells of amphibians (Clothier et al 1978;Braun and Dantzler 1997;Møbjerg et al 2000;Uchiyama and Yoshizawa 2002). On the other hand, the primary features of the amphibian diluting segment are deep basolateral infoldings and elongated mitochondria occupying the cytoplasm within these infoldings, characteristics of cells of the thick ascending limb of the Henle's loop in mammalian kidney (Braun and Dantzler 1997;Møbjerg et al 2000;Uchiyama and Yoshizawa 2002).…”

Section: Nhe3 Expression During Ontogenymentioning

confidence: 99%

Cellular localization of a putative Na+/H+ exchanger 3 during ontogeny in the pronephros and mesonephros of the Japanese black salamander (Hynobius nigrescens Stejneger)

et al. 2007

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The cloning of cDNA and an examination of the tissue distribution of Na(+)/H(+) exchanger 3 (NHE3) were carried out in the Japanese black salamander, Hynobius nigrescens. The cellular localization of Hynobius NHE3 was examined by in situ hybridization and immunohistochemistry during ontogeny in the nephron of the pronephros and mesonephros of the salamander. The partial amino acid sequence of Hynobius NHE3 was 81% and 72% identical to rat NHE3 and stingray NHE3, respectively. Hynobius NHE3 mRNA and protein were exclusively expressed along the late portion of the distal tubule to the anterior part of the pronephric duct of premetamorphic larvae (IY stages 43-50). NHE3 mRNA was expressed in the pronephros but not in the external gills in the larvae at the digit differentiation stage (IY stage 50). In the adult, mRNA was strongly expressed in the mesonephros but not in the ventral and dorsal skin. In juvenile and adult specimens, NHE3 immunoreactivity was observed at the apical membrane of the initial parts of the distal tubules of the mesonephric kidney. Immunohistochemical and in situ hybridization studies suggested that Na(+) absorption coupled with H(+) secretion via NHE3 occurred in the distal nephron of the pronephros and mesonephros. This is the first study to indicate NHE3 expression during ontogeny in amphibians.

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Microanatomy and ultrastructure of the kidney of the African lungfish Protopterus dolloi

et al. 2006

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The Dipnoi (lungfishes) have developed true lungs, having the ability to take oxygen from both the gills and the lungs. During the tropical dry season, many lungfish estivate on land, breathing only air. The estivation period is accompanied by profound functional modifications, including the suppression of urine. Thus, the lungfish kidney must be designed to cope with these dramatic cyclic changes in renal function. We study here the microanatomy and the structure of the kidney of the African lungfish Protopterus dolloi, maintained under controlled freshwater conditions. Chemical microdissection, light microscopy, and scanning and transmission electron microscopy have been used. The nephrons of P. dolloi are composed of a renal corpuscle (RC) and of a renal tubule that appears divided into five morphologically distinct segments: neck segment (NS), proximal tubule (PT), intermediate segment (IS), distal tubule (DT), and collecting tubule (CT). Paired CTs abut into a collecting duct, the latter emptying into an archinephric duct. The RCs lie in the mid-zone of the kidney, between the PTs and the convoluted DTs. The spatial distribution of these elements allows recognition of a kidney zonation. The RCs group into clusters (3-4 RCs per cluster) that are supplied by a single arteriole surrounded by pericytes. Each cluster appears to represent a functional unit with a common hemodynamic regulatory mechanism. The major processes of the podocytes form flattened networks that appear to constitute an integrated system due to the presence of gap junctions. The existence of mesangial cells with large cell processes, and of mesangial cells with a dendritic appearance, suggests a complex functional role (contractile and phagocytic) for the mesangium. The NS and the IS are the narrowest nephron segments, formed only by multiciliated cells. The PT and the DT can be subdivided, based on the tubular morphology and on cell composition, into portions I and II: PTI is formed only by brush border (BB) cells, while PTII contains BB and multiciliated cells. The DTI is formed by segment-specific cells, while the DTII contains segment-specific and a small number of flask cells. The CT contains principal and flask cells in a 5:1 ratio. The flask cells adopt two different configurations (with a narrow canaliculus or with a large cavity). The main goal of this study was to disclose specific kidney features that could be related to function, phylogeny, and habitat. In addition, the present results constitute the basis for a study of the morphologic changes that should occur in the kidney of P. dolloi during estivation. Anat Rec Part A, 288A: 609 -625, 2006.

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Morphology of the kidney in larvae ofBufo viridis (Amphibia, Anura, Bufonidae)

Cited by 43 publications

References 18 publications

The zebrafish pronephros: A model to study nephron segmentation

The zebrafish pronephros: A model to study nephron segmentation

Cellular localization of a putative Na+/H+ exchanger 3 during ontogeny in the pronephros and mesonephros of the Japanese black salamander (Hynobius nigrescens Stejneger)

Microanatomy and ultrastructure of the kidney of the African lungfish Protopterus dolloi

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