Morphology and thermal stability of silk fibroin/starch blended microparticles

Baimark, Yodthong; Srisa-ard, Mangkorn; Srihanam, Prasong

doi:10.3144/expresspolymlett.2010.94

Cited by 25 publications

(18 citation statements)

References 26 publications

(32 reference statements)

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“…loss of Dox-loaded spheres, observed till 100 ºC, could be attributed to the evaporation of water from the sample, which was mentioned in previous studies for silk based structures [66][67][68]. The main weight loss of H21MS1:MS1Fe1/IONPs/Dox particles took place in the temperature range of 250-750 ºC which may be associated with the decomposition of the side chain groups of the amino acid residues and the breakdown of peptide bonds [69].…”

Section: Discussionmentioning

confidence: 54%

Hyperthermia treatment of cancer cells by the application of targeted silk/iron oxide composite spheres

Kucharczyk

Kaczmarek

Józefczak

et al. 2021

Materials Science and Engineering: C

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Magnetic iron oxide nanoparticles (IONPs) are one of the most extensively studied materials for theranostic applications. IONPs can be used for magnetic resonance imaging (MRI), delivery of therapeutics, and hyperthermia treatment. Silk is a biocompatible material and can be used for biomedical applications. Previously, we produced spheres made of H2.1MS1 bioengineered silk that specifically carried a drug to the Her2-overexpressing cancer cells. To confer biocompatibility and targeting properties to IONPs, we blended these particles with bioengineered spider silks.Three bioengineered silks (MS1Fe1, MS1Fe2, and MS1Fe1Fe2) functionalized with the adhesion peptides F1 and F2, were constructed and investigated to form the composite spheres with IONPs carrying a positive or negative charge. Due to its highest IONP content, MS1Fe1 silk was used to produce spheres from the H2.1MS1:MS1Fe silk blend to obtain a carrier with cell-targeting properties. Composite H2.1MS1:MS1Fe1/IONP spheres made of silks blended at different ratios were obtained. Although the increased content of MS1Fe1 silk in particles resulted in an increased affinity of the spheres to IONPs, it decreased the binding of the composite particles to cancer cells. The H2.1MS1:MS1Fe1 particles prepared at a ratio of 8:2 and loaded with IONPs exhibited the ability to bind to the targeted cancer cells similar to the control spheres without IONPs. Moreover, when exposed to the alternating magnetic field, these particles generated 2.5 times higher heat. They caused an almost three times higher percentage of apoptosis in cancer cells than the control particles.The blending of silks enabled the generation of cancer-targeting spheres with a high affinity for iron oxide nanoparticles, which can be used for anti-cancer hyperthermia therapy.

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Section: Discussionmentioning

confidence: 54%

Hyperthermia treatment of cancer cells by the application of targeted silk/iron oxide composite spheres

Kucharczyk

Kaczmarek

Józefczak

et al. 2021

Materials Science and Engineering: C

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“…The mixture then was padded on the fabric, the samples were dried at 105 °C to support coagulation and then were rinsed with water and dried. A temperature of 105 °C was chosen for drying as stability of fibroin is known to allow thermal treatments up to 150 °C . Fibroin deposition/fixation was assessed by direct observation of the color in case of RB19 marked fibroin.…”

Section: Resultsmentioning

confidence: 99%

“…A temperature of 105 8C was chosen for drying as stability of fibroin is known to allow thermal treatments up to 150 8C. 42 Fibroin ARTICLE WILEYONLINELIBRARY.COM/APP deposition/fixation was assessed by direct observation of the color in case of RB19 marked fibroin. In case of undyed fibroin staining with Comassie Blue was used to visualize the presence of the fibroin coating.…”

Section: Fibroin Regenerationmentioning

confidence: 99%

Surface modification of textile material through deposition of regenerated silk fibroin

Ngo

Bechtold

2017

J of Applied Polymer Sci

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Regeneration of silk fibroin from calcium chloride/ethanol/water solution is of high interest to shape biomaterial based products for medical and technical application. In this study a continuous process for surface modification of cellulose or polyamide fibers by regenerated fibroin deposits has been investigated. The decomposition of the fibroin‐calcium complex was initiated by addition of K2CO3 followed by a methanol rinse. Reactive Blue 19 labeled fibroin was used to monitor the deposition of fibroin on the substrates by colour measurement. The fibroin deposits on the fabric were characterized by microscopy, N‐content, calcium, and protein content. Stiffness and surface resistivity of modified fabrics were determined as representative physical parameters. The reduced mobility of fibers increased fabric stiffness. Surface resistivity of treated samples was reduced by a factor of 10 to 100, which gives an example for the potential of the technique as coating for man‐made fiber textiles. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45098.

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“…In addition, the position of amide I shifted, indicating that the hydroxyl group of alginate interacted with keratin. e Alg microparticle showed predominant broad peaks around 3399-2930 cm −1 due to O-H and C-H stretching and about 1038 cm −1 which is the saccharide backbone structure [21]. is revealed that the forces affected to form the Ker/Alg blend microparticles were hydrogen and negatively charged electrostatic forces [20].…”

Section: Secondary Structurementioning

confidence: 91%

Preparation and Characterization of Keratin/Alginate Blend Microparticles

Srisuwan

Srihanam

2018

Advances in Materials Science and Engineering

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The water-in-oil (W/O) emulsification-diffusion method was used for construction of keratin (Ker), alginate (Alg), and Ker/Alg blend microparticles. The Ker, Alg, and Ker/Alg blend solutions were used as the water phase, while ethyl acetate was used as the oil phase. Firstly, different concentrations of Ker solution was used to find suitable content. 1.6% w/v Ker solution was blended with the same concentration of the Alg solution for further microparticle construction. Results from scanning electron microscope analysis show that the microparticles have different shapes: spherical, bowl-like, porous, and hollow, with several sizes depending on the blend ratio. FTIR and TG analyses indicated that the secondary structure and thermal stability of the microparticles were influenced by the Ker/Alg blend ratio. The interaction between functional groups of keratin and alginate was the main factor for both β-sheet structure and Td,max values of the microparticles. The results suggested that Ker/Alg blend microparticles might be applied in many fields by varying the Ker/Alg ratio.

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Morphology and thermal stability of silk fibroin/starch blended microparticles

Cited by 25 publications

References 26 publications

Hyperthermia treatment of cancer cells by the application of targeted silk/iron oxide composite spheres

Hyperthermia treatment of cancer cells by the application of targeted silk/iron oxide composite spheres

Surface modification of textile material through deposition of regenerated silk fibroin

Preparation and Characterization of Keratin/Alginate Blend Microparticles

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