Morphology and molecular phylogeny of the anaerobic freshwater ciliate Urostomides spinosus nov. spec. (Ciliophora, Armophorea, Metopida) from China

Zhuang, Wenbao; Li, Song; Bai, Yang; Zhang, Tengteng; Al‐Rasheid, Khaled A. S.; Hu, Xiaozhong

doi:10.1016/j.ejop.2021.125823

Cited by 16 publications

(6 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…which is consistent with previous studies to some extent [20,26,42]. The sister relationship between Caudidiodeviata nantongensis nov. gen., nov. sp.…”

Section: Phylogenetic Analysessupporting

confidence: 93%

Integrative data of an unknown ciliate (Alveolata, Ciliophora) propose the establishment of Caudidiodeviata nov. gen.

Liao,

Jiang,

2023

Preprint

Self Cite

View full text Add to dashboard Cite

Background As unicellular eukaryotes, ciliates are an indispensable component of ecosystems that play the role of intermediate nutrition link between bacteria or algae and meifauna. Recent faunistic surveys have discovered many new taxa of hypotrich ciliates, indicating their diversity is greater than previously thought. Here we document an undescribed form isolated from an artificial brackish water pond in East China. Examination of its morphology, ontogenesis and molecular phylogeny suggests it represents a new genus. Results The new genus is distinguished from its relatives by having a dorsomarginal kinety, frontoventral and caudal cirri. It also has five frontoventral cirral anlagen, and oral primordium originated apokinetally between right and left cirral field. The diagnostic traits of the new species include three frontal cirri, one buccal cirrus, one or two parabuccal cirri, a row of four to six frontoventral cirri, three left and two right marginal rows, and one bipolar dorsal kinety with one or two caudal cirri at its rear end. Its main morphogenetic features are: (i) the old oral apparatus is completely inherited by the proter except undulating membranes, which are reorganized in situ; (ii) anlagen for marginal rows and the left dorsal kinety develop intrakinetally in both proter and opisthe; (iii) dorsal kinety 2 is generated dorsomarginally; (iv) in the proter, anlagen I and II originate from the parental undulating membranes and the buccal cirrus, respectively, anlage III from anterior parabuccal cirrus, anlage IV from the parental frontoventral cirri; (v) anlagen I–IV of the opisthe are all generated from oral primordium, and (vi) anlage V occur in the innermost parental right marginal row in both dividers. The phylogenetic analysis suggests that the new species is closely related to the newly described Heterodeviata sinica Song et al., 2023, and then form a robust clade with other deviatids, which corroborates the familial assignment of our form. Conclusions The study of the morphology, and ontogenesis of a new brackish-water taxon increases the overall knowledge about the biodiversity of this ciliate group. It also adds to the genetic data available and further confirms the monophyly of family Deviatidae Foissner, 2016.

show abstract

“…which is consistent with previous studies to some extent [20,26,42]. The sister relationship between Caudidiodeviata nantongensis nov. gen., nov. sp.…”

Section: Phylogenetic Analysessupporting

confidence: 93%

Integrative data of an unknown ciliate (Alveolata, Ciliophora) propose the establishment of Caudidiodeviata nov. gen.

Liao,

Jiang,

2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…the Metopus-Brachonella clade. Once again, the present study supports the hypothesis that Metopus is not monophyletic Li et al, 2016Li et al, , 2021Omar et al, 2017;Paiva et al, 2013;Rotterová et al, 2018;Silva-Neto et al, 2016;Zhuang et al, 2021).…”

Section: Phylogeny and The Paraphyly Of Castulasupporting

confidence: 89%

“…With the growing interest in discovering the diversity and important ecological function of anaerobic archaea, anaerobic ciliates as their main hosts also have become a matter of great concern recently (Bourland et al., 2017a; Li et al., 2020; Rotterová et al., 2022; Seitz et al., 2019; Zhang et al., 2021; Zhuang et al., 2021). The order Metopida Jankowski, 1980, consisting of three families, i.e.…”

Section: Introductionmentioning

confidence: 99%

Morphology, morphogenesis, and molecular characterization of Castula specialis sp. nov. (Ciliophora, Armophorea, Metopida)

Feng,

Méndez‐Sánchez,

Zhuang

et al. 2023

J Eukaryotic Microbiology

Self Cite

View full text Add to dashboard Cite

The morphology, morphogenesis, and molecular phylogeny of a new metopid ciliate, Castula specialis sp. nov., comprising three strains from geographically distant (China, Mexico, Czech Republic) anoxic freshwater habitats, were studied based on microscopic observation of live and protargol‐stained specimens as well as SSU rRNA gene sequence data. The new species is characterized as follows: size in vivo 105–220 × 25–70 μm, body oblong to elongated ellipsoidal and asymmetrical; preoral dome distinctly projecting beyond the body; 32–46 adoral membranelles; 31–52 somatic kineties; and 4–7 setae. This study brings the first morphogenetic investigation of a member of the genus Castula. The morphogenesis of the type population (China) of the new species proceeds as in Metopus spp. comprising drastic changes in body shape and a pleurotelokinetal stomatogenesis; however, the main difference is the origin of the opisthe's paroral membrane that derives from all perizonal rows and some adjacent dome kineties. Phylogenetically, the genus Castula is paraphyletic.

show abstract

“…The overall topology of the phylogenetic tree (Figure 8) is consistent with recent 18S rRNA gene-based analyses (e.g. Bourland et al, 2020;Li et al, 2023;Méndez-Sánchez et al, 2022;Zhuang et al, 2021Zhuang et al, , 2022. The backbone of the phylogenetic tree was mostly statistically unsupported, but the major clades, that is the main previously identified clades of the Metopida/Clevelandellida lineage: MAC (named after included taxa Metopus, Atopospira, Clevelandellida), HAC (Heterometopus, Atopospira, Clevelandellida), LAC (Longitaenia, Atopospira, Clevelandellida), IAC, and Clevelandellida (Bourland et al, 2018), were recovered, and fully or well supported.…”

Section: Phylogenetic Analysissupporting

confidence: 86%

Bit by bit toward the diversity of metopids: Description of the genus Pidimetopus n. gen. (Ciliophora: Armophorea)

Pomahač,

Méndez‐Sánchez,

Čepička

2024

J Eukaryotic Microbiology

View full text Add to dashboard Cite

While metopids (Armophorea: Metopida) represent the most species‐rich group of free‐living anaerobic ciliates thriving in hypoxic environments, our understanding of their true diversity remains incomplete. Most metopid species are still characterized only morphologically. Particularly, the so‐called IAC clade (named in the past after some of the taxa included, Idiometopus, Atopospira, and Clevelandellida), comprising free‐living members as well as the endosymbiotic ones (order Clevelandellida), is in serious need of revision. In our study, we establish a new free‐living genus in the IAC clade, Pidimetopus n. gen., with descriptions of two new species, P. nanus n. sp., and P. permonicus n. sp., using up‐to‐date molecular and morphologic methods. The genus is characterized by small cells (up to 75 μm long), not more than 10 adoral membranelles and eight somatic kineties, and usually, four long caudal cilia that can stiffen. In addition to morphologic and molecular characterizations, we also conducted a statistical morphotype analysis of the polymorphic species P. nanus n. sp. We discuss the relevance of the earlier morphologically described species Metopus minor as a putative collective taxon for several small metopids less than 50 μm long.

show abstract

Morphology and molecular phylogeny of the anaerobic freshwater ciliate Urostomides spinosus nov. spec. (Ciliophora, Armophorea, Metopida) from China

Cited by 16 publications

References 29 publications

Integrative data of an unknown ciliate (Alveolata, Ciliophora) propose the establishment of Caudidiodeviata nov. gen.

Integrative data of an unknown ciliate (Alveolata, Ciliophora) propose the establishment of Caudidiodeviata nov. gen.

Morphology, morphogenesis, and molecular characterization of Castula specialis sp. nov. (Ciliophora, Armophorea, Metopida)

Bit by bit toward the diversity of metopids: Description of the genus Pidimetopus n. gen. (Ciliophora: Armophorea)

Contact Info

Product

Resources

About