2009
DOI: 10.1111/j.1439-0531.2008.01265.x
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Abstract: The objective was to investigate whether it is possible to improve the quality of stallion semen, with respect to sperm morphology and chromatin integrity, both of which have been linked to fertility, using either density gradient centrifugation (DGC) or a new method, hereby named single layer centrifugation (SLC). The two methods of colloidal centrifugation were evaluated using 38 ejaculates from 10 stallions. Sperm morphology, subjective motility and sperm chromatin integrity were compared in uncentrifuged s… Show more

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Cited by 78 publications
(76 citation statements)
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“…However, for one stallion (V), DFI was far higher in the centrifuged samples than in the uncentrifuged samples. This result was surprising and is in contrast to other results, both with fresh sperm samples (Morrell et al, 2009c) and with frozen sperm samples (Macias et al, 2009). The reason for this high DFI is unknown.…”
Section: Discussioncontrasting
confidence: 99%
“…However, for one stallion (V), DFI was far higher in the centrifuged samples than in the uncentrifuged samples. This result was surprising and is in contrast to other results, both with fresh sperm samples (Morrell et al, 2009c) and with frozen sperm samples (Macias et al, 2009). The reason for this high DFI is unknown.…”
Section: Discussioncontrasting
confidence: 99%
“…In accordance with previous work (Mengual et al 2003), a significant difference in viability and evident difference of subjective motility could be detected between these fractions and the first '40-70% interphase' (data not shown). Centrifugation through gradient density increased homogeneity of the preparation as reported (Morrell et al 2008). The viability assays were performed in parallel with a control sample for which spermatozoa were treated with Triton X-100 0.3% for 1 min.…”
Section: Sperm Recovery and Viabilitymentioning
confidence: 99%
“…Androcoll-E TM has been used to select robust spermatozoa in terms of motile and morphologically normal sperm, with intact membranes and good chromatin integrity (Morrell et al, 2009c). On the basis of all this sperm parameters to perform the sperm quality analysis, Androcoll-E has improved the quality of fresh (Morrell et al, 2009a), frozen (Macias Garcia et al, 2009a;Macias Garcia et al, 2009b;Hoogewijs et al, 2011) and cooled-stored (Morrell et al, 2009d;Bergqvist et al, 2011) stallion sperm samples with a shorter preparation time and less complicated process than the conventional density gradient centrifugation . However, a specific formulation for donkey semen has not been developed yet.…”
Section: Introductionmentioning
confidence: 99%