Morphological risk assessment of cold atmospheric plasma-based therapy: bone marrow mesenchymal stem cells in treatment zone proximity

Abstract

Cold atmospheric plasma (CAP) is a 'partially ionized' gas composed of free electrons, positive and negative ions, radicals, excited species, an electric field, and ultraviolet radiation (UVR) [1][2][3][4]. One of the most important advantages of cold plasma is its ability to work at room temperature and atmospheric pressure, which makes it possible to be utilized in biological media with minimal thermal side effects [5][6][7][8]. In this regard, the prominent applications of CAP are microorganism

A van der Waals force-based adhesion study of stem cells exposed to cold atmospheric plasma jets

“…Before trypsinization, cells were cultured at 37℃ for 24 hours to prepare them for the de-adhesion assay. Petri dishes were exposed directly to the plasma output of the CAP system, ignited at both high (35W, representing a high risk area [37]) and low (20W, representing a safe area [37]) power ranges of the generator. Finally, varied doses of plasma-activated water, PAW, were applied to some of the coverslips.…”

“…Finally, varied doses of plasma-activated water, PAW, were applied to some of the coverslips. As previously stated [37], the PAW has been classified as; 1) high dose: the power of plasma generator 35W and the exposure time of 90s and, 2) low dose: the power of plasma generator 20W with exposure time less than 90s. After treatment, cells were incubated under the same conditions for another twenty-four hours.…”

“…The Argon-fed DBD-plasma Jet of Kharazmi University-Plasma lab used to treat Stem Cells is an RF (13.56MHz) Plasma Jet, see our previous paper [37]. Its power set to 20W and 35W (as no-risk and high-risk limits of generators power for treating the stem cells [37]) and gas flow rate of 1slm. The bulk plasma temperature has been measured to 37℃.…”

“…The bulk plasma temperature has been measured to 37℃. The spectrum of our plasma can be found in reference [37] which shows the generation of the some important reactive species generated with this device (OH, O, NO).…”

“…Cells were stained with DAPI (4 ,6-diamidino-2-phenylindole) according to "Cold Harbor Spring"protocol (see ref. [37] for details) to follow the cell dynamics and shapes. Briefly, cultured cells were washed, twice, with phosphate-buffered saline (PBS), fixed in 4% paraformaldehyde for 10min, and permeabilized with 0.5% Triton X-100 for 15min at room temperature.…”