Morphological evaluation of osteoblasts cultured on different calcium phosphate ceramics

Knabe, Christine; Gildenhaar, Renate; Berger, Georg; Ostapowicz, W.; Fitzner, Rudolf; Radlanski, Ralf J.; Groß, Ulrich

doi:10.1016/s0142-9612(97)00078-1

Cited by 54 publications

(45 citation statements)

References 18 publications

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“…Besides surface energy considerations, Knabe et al related a relative inhibition of osteoblast growth in vitro with a relatively high phosphate-ion release. 44,45 This observation is quite in accord with the present study: OCP has an atomic calcium to phosphorus ratio (Ca/P) of 1.33 whereas BCA has a Ca/P ϭ 1.60. 29 In addition BCA dissolves faster than OCP in the condyle and therefore has a higher phosphate-ion release with a BCA coating than it has with an OCP coating.…”

Section: Discussionsupporting

confidence: 92%

Osteogenecity of octacalcium phosphate coatings applied on porous metal implants

Barrère

Valk

Dalmeijer

et al. 2003

J Biomedical Materials Res

235

201

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The biomimetic route allows the homogeneous deposition of calcium phosphate (Ca-P) coatings on porous implants by immersion in simulated physiologic solution. In addition, various Ca-P phases, such as octacalcium phosphate (OCP) or bone-like carbonated apatite (BCA), which are stable only at low temperatures, can be deposited. In this pilot study, experiments were designed with a twofold-purpose: (1) to investigate the osteoinduction of OCP-coated and noncoated porous tantalum cylinders and of dense titanium alloy cylinders (5 mm in diameter and 10 mm in length) in the back muscle of goats at 12 and 24 weeks (n = 4); and (2) to compare the osteogenic potentials of BCA-coated, OCP-coated, and bare porous tantalum cylinders in a gap of 1 mm created in the femoral condyle of a goat at 12 weeks (n = 2). In the goat muscle, after 12 weeks the OCP-coated porous cylinder had induced ectopic bone as well as bone within the cavity of the OCP-coated dense titanium cylinder. In the femoral condyle, bone did not fill the gap in any of the porous implants. In contrast with the two other groups, OCP-coated porous cylinders exhibited bone formation in the center of the implant. The nature of the Ca-P coating, via its microstructure, its dissolution rate, and its specific interactions with body fluids, may influence the osteogenecity of the Ca-P biomaterial.

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Section: Discussionsupporting

confidence: 92%

Osteogenecity of octacalcium phosphate coatings applied on porous metal implants

Barrère

Valk

Dalmeijer

et al. 2003

J Biomedical Materials Res

235

201

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“…It has been reported that calcium ions in HAp, which form sites of positive charge, aid the adhesion of these two proteins, thus promoting the attachment of osteoblasts [45,46]. Other researchers have also reported the positive effect of calcium ions on cell growth [47,48], in contrast to the inhibitory effect of phosphate ions on cell activity [49]. Thus, the enhanced bioactivity of HAp6.0 compared to HAp4.2 may be explained in the combined effect of crystallinity and Ca content.…”

Section: Discussionmentioning

confidence: 99%

The effect of surface treatment on the surface texture and contact angle of electrochemically deposited hydroxyapatite coating and on its interaction with bone-forming cells

et al. 2009

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“…In this way, cell viability can be maintained and cell proliferation and differentiation of the osteoblast-like cells improved. 39 In addition to the decrease in pH and the uptake of calcium by the specimens, the negative effects on osteoblastlike cells could be due to the particle size of the CaP cement. Pioletti et al described that CaP cement particles smaller than 10 mm have an effect on the viability and proliferation of osteoblast-like cells.…”

Section: Discussionmentioning

confidence: 99%

The Cytocompatibility and Early Osteogenic Characteristics of an Injectable Calcium Phosphate Cement

et al. 2007

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In this study, the cytocompatibility and early osteogenic characteristics of rat bone marrow cells (RBMCs) on injectable calcium phosphate (CaP) cement (Calcibon) were investigated. In addition to unmodified CaP cement discs, 2 other treatments were given to the discs: preincubation in MilliQ and sintering at different temperatures. After primary culture, RBMCs were dropwise seeded on the discs and cultured for 12 days. The samples were evaluated in terms of cell viability, morphology (live and dead assays and scanning electron microscopy (SEM)), cell proliferation (deoxyribonucleic acid (DNA) analyses), early cell differentiation (alkaline phosphatase (ALP) activity), and physicochemical analyses (xray diffraction (XRD)). The live and dead, DNA, and SEM results showed that Calcibon discs without any additional treatment were not supporting osteoblast-like cells in vitro. There were fewer cells, and cell layers were detached from the disc surface. Therefore, different preincubation periods and sintering temperatures were evaluated to improve the cytocompatibility of the CaP cement. Preincubating discs in MilliQ for periods of 1, 4, 8, and 12 weeks resulted in the hydrolysis of a-tri calcium phosphate (TCP) into an apatite-like structure with some b-TCP, as shown with XRD, but the material was not cytocompatible. Sintering the discs between 8008C and 11008C resulted in conversion of a-TCP to b-TCP with some hydroxyapatite and an increase in crystallinity. Eventually, the discs sintered at 11008C achieved better cell attachment, more-abundant cell proliferation, and earlier differentiation than other sintered (6008C, 8008C, and 10008C), preincubated, and unmodified specimens. On basis of our results, we conclude that in vivo results with CaP-based cements do not guarantee in vitro applicability. Furthermore, unmodified Calcibon is not cytocompatible in vitro, although preincubation of the material results in a more-favorable cell response, sintering of the material at 11008C results in the best osteogenic properties. In contrast to in vivo studies, the Calcibon CaP cement is not suitable as a scaffold for cellbased tissue-engineering strategies.

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Morphological evaluation of osteoblasts cultured on different calcium phosphate ceramics

Cited by 54 publications

References 18 publications

Osteogenecity of octacalcium phosphate coatings applied on porous metal implants

Osteogenecity of octacalcium phosphate coatings applied on porous metal implants

The effect of surface treatment on the surface texture and contact angle of electrochemically deposited hydroxyapatite coating and on its interaction with bone-forming cells

The Cytocompatibility and Early Osteogenic Characteristics of an Injectable Calcium Phosphate Cement

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