Morphological development of the testicles and spermatogenesis in guinea pigs (Cavia porcellus Linnaeus, 1758)

Abstract

Introduction: Understanding the dynamics of spermatogenesis is crucial to clinical andrology and to understanding the processes which define the ability to produce sperm. However, the entire process cannot be modeled in vitro and guinea pig may be an alternative as animal model for studying human reproduction. Objective: In order to establish morphological patterns of the testicular development and spermatogenesis in guinea pigs, we examined testis to assess changes in the testis architecture, transition time from spermatocytes to elongated spermatids and stablishment of puberty. Materials and methods: We used macroscopic analysis, microstructural analysis and absolute measures of seminiferous tubules by light microscopy in fifty-five guinea pigs from one to eleven weeks of age. Results: Differences in relation to mass, length and width of the testes appeared at week 3 and were intensified in week 6 with the puberty. At week 2 the first spermatocytes at preleptotene/leptotene appeared, indicating the onset of meiosis. At week 6 the seminiferous tubules formed lumen, differentiated Sertoli cells and all kinds of germ cells; spermatozoa released into the lumen and the largest expansion of tubular diameter and height of the seminiferous epithelium occurred. Transition time from spermatocytes to elongated spermatids was estimated in 21 days. Conclusion: We conclude that the testicular development and puberty are faster than in other mammal and rodent species, demonstrating the suitability of the guinea pig testes as a model for studies of the testicular development and spermatogenesis.

The Effect of Kuchala (Arum Korolkowii Regel, 1873) Tuber Tincture to Increase of the Serum Testosterone in the Adult Male Guinea Pigs (Cavia Porcellus Linnaeus, 1758)

“…Importantly, the guinea pig has many common features with humans in terms of reproduction related to the accessory glands [14,15], the characteristics of the placenta [21], and morphological and functional analysis of spermatogenesis [22]. In addition, information exits that the testosterone and androstenedione content in serum and testes were different in guinea pigs in the prenatal [18,23], and postnatal [24] periods. The concentration of testosterone in plasma reaches its maximum level at 60 days of age in guinea pigs, and then decreases with the increase in age.…”

“…Guinea pigs have been comprehensively studied as laboratory animals using biological, morphological and physiological approaches. Its size and lifestyle make the guinea pig easy to keep and to conduct experimental studies on and, importantly, it has many of the same morphological and physiological characteristics as humans [11,12], including in terms of reproduction [13][14][15][16][17][18]. Consequently, guinea pigs are frequently used as a biological model in studies of a number of infectious bacterial and noninfectious diseases [12,19].…”

The Effect of Kuchala (Arum Korolkowii Regel, 1873) Tuber Tincture to Increase of the Serum Testosterone in the Adult Male Guinea Pigs (Cavia Porcellus Linnaeus, 1758)

“…Importantly, the guinea pig has many common features with human reproduction -by presenting of the accessory glands [16,17], characteristics of the placenta [25], morphological and functional analysis of spermatogenesis [26]. Besides, it has available information that the content of testosterone and androstenedione in serum and testis were different in guinea pigs in the prenatal [20,27], and postnatal [28] periods. The concentration of testosterone in plasma reaches its maximum level at 60 days of age in guinea pigs, and then testosterone decreases with the increase of age.…”

“…Guinea pig was comprehensive studied as laboratory animal in biological, morphological and physiological approaches. Its size and lifestyle make the guinea pig easy to keep and conducts experimental study, and importantly, it has many same morphological and physiological characteristics with human [13,14], including in reproduction [15,16,17,18,19,20].…”

Stereological and computed tomography calculation of intracranial volume in Hamdani sheep

“…The literature incorporates no studies on intracranial volume in Hamdani sheep. There are volumetric studies on species such as gazelle Demircioğlu, Koçyiğit, Demiraslan, et al, 2021), deer (Logan & Clutton-Brock, 2013), goat (Rodrigues et al, 2017), antelope (Choudhary & Singh, 2015) and sheep (Karimi et al, 2011). The aim of this study is to construct 3-dimensional cranial cavity models using 2-dimensional CT sections.…”