Morphological Changes in the Colon and Composition of Peripheral Blood Lymphocytes in Acute Colitis in Mice With Different Resistance to Hypoxia

Джалилова, Д. Ш.; Polyakova, Margarita; Diatroptov, M. E.; Золотова, Н.А.; Макарова, О.В.

doi:10.29296/24999490-2018-06-08

Cited by 9 publications

(3 citation statements)

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“…According to our previous data, tolerant to hypoxia rats have more pronounced cellular and innate immunity, while humoral immunity is more pronounced in susceptible animals 127 . In addition, in susceptible to hypoxia animals, a more severe course of the systemic inflammatory response, acute and chronic ulcerative colitis were revealed 45 , 128 , 129 . Therefore, it is necessary to conduct additional studies on the tumor process features in animals with different resistance to hypoxia, which will allow to reveal mechanisms and to develop new personalized approaches to tumor therapy.…”

Section: Discussionmentioning

confidence: 99%

Morphological and molecular-biological features of glioblastoma progression in tolerant and susceptible to hypoxia Wistar rats

Джалилова

Золотова

Мхитаров

et al. 2023

Sci Rep

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Hypoxia is a major pathogenetic factor in many cancers. Individual resistance to suboptimal oxygen availability is subject to broad variation and its possible role in tumorigenesis remains underexplored. This study aimed at specific characterization of glioblastoma progression in male tolerant and susceptible to hypoxia Wistar rats. Hypoxia resistance was assessed by gasping time measurement in an 11,500 m altitude-equivalent hypobaric decompression chamber. Based on the outcome, the animals were assigned to three groups termed ‘tolerant to hypoxia’ (n = 13), ‘normal’, and ‘susceptible to hypoxia’ (n = 24). The ‘normal’ group was excluded from subsequent experiments. One month later, the animals underwent inoculation with rat glioblastoma 101.8 followed by monitoring of survival, body weight dynamics and neurological symptoms. The animals were sacrificed on post-inoculation days 11 (subgroup 1) and 15 (subgroup 2). Relative vessels number, necrosis areas and Ki-67 index were assessed microscopically; tumor volumes were determined by 3D reconstruction from histological images; serum levels of HIF-1α, IL-1β, and TNFα were determined by ELISA. None of the tolerant to hypoxia animals died of the disease during observation period, cf. 85% survival on day 11 and 55% survival on day 15 in the susceptible group. On day 11, proliferative activity of the tumors in the tolerant animals was higher compared with the susceptible group. On day 15, proliferative activity, necrosis area and volume of the tumors in the tolerant to hypoxia animals were higher compared with the susceptible group. ELISA revealed no dynamics in TNFα levels, elevated levels of IL-1β in the susceptible animals on day 15 in comparison with day 11 and tolerant ones. Moreover, there were elevated levels of HIF-1α in the tolerant animals on day 15 in comparison with day 11. Thus, the proliferative activity of glioblastoma cells and the content of HIF-1α were higher in tolerant to hypoxia rats, but the mortality associated with the tumor process and IL-1β level in them were lower than in susceptible animals. Specific features of glioblastoma 101.8 progression in tolerant and susceptible to hypoxia rats, including survival, tumor growth rates and IL-1β level, can become the basis of new personalized approaches for cancer diseases treatment in accordance to individual hypoxia resistance.

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Section: Discussionmentioning

confidence: 99%

Morphological and molecular-biological features of glioblastoma progression in tolerant and susceptible to hypoxia Wistar rats

Джалилова

Золотова

Мхитаров

et al. 2023

Sci Rep

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“…Even linear animals have distinct individual differences—in the population of adult male C57BL/6 mice, it is always possible to identify 10–40% of animals with low and high resistance to hypoxia. We have previously demonstrated that, in susceptible-to-hypoxia animals, the course of acute and chronic DSS-induced colitis was much more pronounced than in tolerant-to-hypoxia mice [ 36 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

Influence of Microplastics on Morphological Manifestations of Experimental Acute Colitis

Zolotova,

Dzhalilova,

Tsvetkov

et al. 2023

Toxics

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Microplastic pollution poses a threat to human health. It is possible that the increase in the incidence of inflammatory bowel disease is associated with exposure to microplastics. We investigated the effect of the consumption of polystyrene microparticles with a diameter of 5 μm at a dose of 2.3 mg/kg/day for 6 weeks on morphological changes in the colons of healthy male C57BL/6 mice and of mice with acute colitis induced by a 1% dextran sulfate sodium solution (DSS). In healthy mice, microplastics caused an increase in the number of endocrine cells, an increase in the content of highly sulfated mucins in goblet cells, an increase in the number of cells in the lamina propria, and a decrease in the volume fraction of macrophages. Microplastic consumption caused more severe acute colitis, which is characterized by a greater prevalence of ulcers and inflammation and a decrease in the content of neutral mucins in goblet cells.

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“…It is known, that hypoxia plays a key role in both the system and local inflammatory reactions, mainly due to microcirculatory disorders and disseminated intravascular coagulation [3]. In this regard a number of studies have shown that severity of any inflammatory diseases, including inflammatory diseases of the gut such as Crohn's disease (CD) and ulcerative colitis (UC) depends on hypoxia resistance [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

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2020

Russ Open Med J

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Intestinal bowel diseases (IBD), including ulcerative colitis (UC), is the group of difficult to diagnose widespread among the population diseases. Pathogenesis of the disease is associated with a complex interaction of the genetic factors, the environment, the microbiome and the unpredicted reaction of the immune system, and the existing treatment methods are not effective enough. It is known, that hypoxia plays a key role in both system and local inflammatory reactions, mainly due to microcirculatory disorders and disseminated intravascular coagulation. Therefore a lot of studies have demonstrated that severity of any inflammatory diseases, including Crohn's disease (CD) and UC depends on hypoxia resistance. In this review we discussed microcirculation of blood and physiological hypoxia in the intestine, the role of hypoxia-inducible factors in the development of IBD and UC, as well as their influence on the severity of the inflammatory process. Authors described the protective effect of various PHD inhibitors and its benefits and disadvantages, so as new approaches of searching of very specific low molecular weight substanses as drugs for the control of IBD and UC.

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Morphological Changes in the Colon and Composition of Peripheral Blood Lymphocytes in Acute Colitis in Mice With Different Resistance to Hypoxia

Cited by 9 publications

References 0 publications

Morphological and molecular-biological features of glioblastoma progression in tolerant and susceptible to hypoxia Wistar rats

Morphological and molecular-biological features of glioblastoma progression in tolerant and susceptible to hypoxia Wistar rats

Influence of Microplastics on Morphological Manifestations of Experimental Acute Colitis

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