Morphological and molecular identification of Isospora massardi Lopes, Berto, Luz, Galvão, Ferreira & Lopes, 2014 (Chromista: Miozoa: Eimeriidae) from thrushes Turdus spp. (Passeriformes: Turdidae) in South America

Isospora basileuterusi n. sp. (Apicomplexa: Eimeriidae) from the golden-crowned warbler Basileuterus culicivorus (Deppe) (Passeriformes: Parulidae) in South America

“…was performed using the cox 1 gene, which is considered to be the gene with the highest resolution in detecting recent speciation events ( Barta, 2001 ; Ogedengbe et al., 2011 ). In fact, the 250 bp cox 1 gene sequence was not 100% similar to any other deposited on GenBank, contrary to what occurs with ribosomal gene sequences that are more conserved and more suitable for phylogenetic studies of families and orders ( Genovez-Oliveira et al., 2020 ). On the other hand, the region of the cox 1 sequenced for I. basileuterusi n. sp.…”

“…(2018) . Perhaps the short sequence of only 250 bp did not allow greater resolution in the phylogenetic study; in this case, sequences with more than 600 bp from other regions of the cox 1 gene, such as those generated by the JAV primer ( Genovez-Oliveira et al., 2020 ), would show better phylogenetic estimations in the future. Fatally, in the present study these JAV primers were not successful in amplifying the samples; however, it has been shown in any case that mitochondrial genes, such as cox 1, are better suited to work with individual oöcysts, as the number of copies of mitochondrial DNA is far greater than the number of copies of nuclear DNA, thus favoring the amplification of mitochondrial genes ( Dolnik et al., 2009 ).…”